Preliminary Phytochemical Screening of Methanolic Extract Of Clerodendron infortunatum

Sheel, Dr.Rimjhim; Kumari, Nisha; Kumar, Jainendra

doi:10.9790/5736-07121013

Cited by 71 publications

(54 citation statements)

References 5 publications

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“…Chloroform layer appeared red and acid layer greenish yellow fluorescent. This confirms the presence of sterols 7 .…”

Section: Preparation Of Earthworm Extractssupporting

confidence: 83%

“…Test for Glycosides: Keller-Kiliani Test: In 2mL extract, glacial acetic acid, one drop of 5% FeCl3 and concentrated H 2 SO 4 were added. Reddish brown colour appears at junction of the two layers and upper layer appears bluish green, confirming the presence of glycosides 7 .…”

Section: Preparation Of Earthworm Extractsmentioning

confidence: 60%

“…Fehling's Test: Fehling A and Fehling B reagents were mixed and few drops of extract are added and boiled. A brick red coloured precipitate of cuprous oxide forms, hence confirming the presence of carbohydrates 7 .…”

Section: Test For Carbohydratesmentioning

confidence: 60%

“…3mL concentrated H 2 SO 4 was carefully added to form a layer. A reddish brown colouration of the interface is formed to show positive result of the presence of terpenoids 7 .…”

Section: Preparation Of Earthworm Extractsmentioning

confidence: 99%

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2017

IJPSR

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This in-vitro antidiabetic and cytotoxic activity of earthworm Lumbricus rubellus extracts were investigated. In the present study, three types of solvent (petroleum ether, 95% ethanol and phosphate buffer) extracts of earthworm paste were prepare and subjected to preliminary screening. The antidiabetic activity was determined in terms of its ability to inhibit the α-amylase activity. Different concentrations ranging from 100-1000µg/mL of earthworm extracts were incubated with enzyme substrate solution and subjected in the determination of absorbance to assess the antidiabetic activity. Also Acarbose was used as the standard inhibitor. The 95% ethanol extracts showed the highest percentage inhibition of α-amylase activity, followed by petroleum ether and phosphate buffer. The IC50 value of 95% ethanol, petroleum ether and phosphate buffer extracts were 242.46µg/mL, 304.15µg/mL and 689.68µg/mL respectively, when compared with Acarbose (IC50 value 193.68µg/mL). The cytotoxic activity was determined towards brine shrimp lethality assay. Different concentrations ranging from 10-100µg/mL of earthworm extracts were subjected to determine the mortality of brine shrimps. Potassium dichromate was used as positive control. Results showed that the petroleum ether extract was potent against the brine shrimp with LC50 values of 7.13µg/mL, followed by 95% ethanol extract (7.28µg/mL) and phosphate buffer extract (73.50µg/mL). While the positive control exhibited LC50 value with 11.96µg/mL. The antidiabetic and cytotoxic activity of the earthworm paste produced by Lumbricus rubellus seems to be a promising new direction in drug delivery research.

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“…Chloroform layer appeared red and acid layer greenish yellow fluorescent. This confirms the presence of sterols 7 .…”

Section: Preparation Of Earthworm Extractssupporting

confidence: 83%

Section: Preparation Of Earthworm Extractsmentioning

confidence: 60%

Section: Test For Carbohydratesmentioning

confidence: 60%

“…3mL concentrated H 2 SO 4 was carefully added to form a layer. A reddish brown colouration of the interface is formed to show positive result of the presence of terpenoids 7 .…”

Section: Preparation Of Earthworm Extractsmentioning

confidence: 99%

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2017

IJPSR

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“…Preliminary qualitative screening for the presence of various phytochemicals was carried out using the protocols of Sheel et al [24], Nanna et al [25], Mishra et al [26], and Sharma et al [27].…”

Section: Phytochemical Screeningmentioning

confidence: 99%

Phytochemicals Screening, Phenolic Estimation and Evaluation for Anti-Oxidant, Anti-Inflammatory and Anti-Microbial Activities of Sequentially Soxhlet Extracted Coconut Testa

Ojha¹,

Roy²,

Das³

et al. 2019

FNS

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Background: In many coconut industries, the outer layer of thin brown skin of coconut kernel known as testa is peeled out as a byproduct. Despite the testa is rich in fat and plenty of polyphenolic compounds, it has been underutilized either as animal feed, serving as raw materials for bio-diesel production or discarded directly. Anticipating coconut testa (CT) as a natural source of multiple phyto-chemicals, its exploitation for the pharmacological activity or utilization as value added product is required which may reduce the disposal costs as well. Methods: Secondary metabolites from CT were extracted sequentially with different organic solvents based on polarity in the soxhlet apparatus followed by extraction with sterilized water. The crude dried extracts thus prepared were evaluated for qualitative screening of phytochemicals and quantitative estimation of total phenols, flavonoids and tannin content. Moreover, the antioxidant, anti-inflammatory and anti-microbial activities were also investigated. Results: Phytochemicals screening revealed the presence of polyphenolic compounds in methanolic fraction including phenols (822.60 ± 16.36 mg/g), flavonoids (103.30 ± 9.78 mg/g) and tannin (663.50 ± 19.26 mg/g), whereas non-phenolic compounds were present in other fractions. While methanolic fraction showed invariably the highest anti-oxidant activity in multiple assay methods, non-phenolic compounds in aqueous and chloroform fractions exhibited high anti-inflammatory activity. Antimicrobial activity was observed by both phenolic and non-phenolic compounds. Conclusion: The findings of the study reveal that CT is a rich source of various polyphenolic and non-phenolic natural antioxidants, anti-inflammatory and antimicrobial compounds. These findings are promising and form the basis to identify the number of active components and their characterization. IntroductionCoconut (Cocos nucifera L.) belongs to the family Arecaceae [Palmae] and the subfamily Cocoideae is an important monocotyledon plant widely grown in the tropic and sub-tropics [1]. Further, certain religious and traditional values of coconut in most parts of India and other South East Asian countries have fared the production and productivity of coconut in these countries much better than many other countries. The coconut palm is sometimes referred to as 'great nut of India' owing to the use of all of its parts in some way or the other in coconut-growing areas. Apart from the use of coconut water, the natural sterile liquid from the young immature coconuts as a beverage; the kernel is used as the source of food and oil. Further, the wastes such as shell or coir are also used as raw materials for many industries for the production of shell-activated carbon or fibers for car seats and other household materials [2] [3] [4]. With the increasing demand for coconut oil, preparation of desiccated coconut powder or other products such as coconut cream and milk powder, the thin brown skin of coconut kernel known as testa is peeled out and processed in many coconu...

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Synthesis and characterization of zinc oxide nanoparticles using peppermint tea (Mentha piperita) dregs extract and their photocatalytic performance

Loh,

Ooi,

Teoh

et al. 2023

Env Prog and Sustain Energy

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Zinc oxide (ZnO) is a n‐type semiconductor with a wide band gap of 3.37 eV and a large excitation binding energy of 60 meV. ZnO nanoparticles (ZnO NPs) is considered as one of the best photocatalyst for the degradation of pollutants in water since it has large surface area to enhance the photocatalytic activity. This article aims to study the feasibility of using the agricultural waste of peppermint tea dregs extract with zinc nitrate hexahydrate as salt precursor to synthesis ZnO NPs through a simple, low cost, greener and environmentally friendly method. The qualitative phytochemical screening of peppermint tea dregs extract revealed the presence of flavonoids which can be served as the reducing and stabilizing agent in the synthesis of ZnO NPs. Various analytical techniques such as FTIR, UV–vis, XRD, FESEM, EDX and PSA confirmed the presence of ZnO NPs. The photocatalytic performance of the synthesized ZnO NPs showed a 95.03% degradation of crystal violet dye within 4 h of sunlight exposure.

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Preliminary Phytochemical Screening of Methanolic Extract Of Clerodendron infortunatum

Cited by 71 publications

References 5 publications

Untitled

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Phytochemicals Screening, Phenolic Estimation and Evaluation for Anti-Oxidant, Anti-Inflammatory and Anti-Microbial Activities of Sequentially Soxhlet Extracted Coconut Testa

Synthesis and characterization of zinc oxide nanoparticles using peppermint tea (Mentha piperita) dregs extract and their photocatalytic performance

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