Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from<i> Ephedra intermedia</i> Indigenous to Balochistan

Gul, Rahman; Jan, Syed Umer; Faridullah, Syed; Sherani, Samiullah; Jahan, Nusrat

doi:10.1155/2017/5873648

Cited by 360 publications

(265 citation statements)

References 18 publications

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“…Phytochemical screening was performed on the dye to ascertain the presence of reducing and capping agent present in the dye extracts. The assays to investigate are; tannins, steroids, phlobatannins, saponins, flavornoids, phenols, anthocyanins and terpenoids and using established methods [30][31][32]33].…”

Section: Phytochemical Analysis Of the Dyementioning

confidence: 99%

Green synthesis of zinc oxide nanoparticles and zinc oxide–silver, zinc oxide–copper nanocomposites using Bridelia ferruginea as biotemplate

et al. 2020

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The quest to synthesize nanomaterials with improved properties, but less undesirable effects on the environment necessitated this research. Zinc Oxide (ZnO) nanoparticles and zinc oxide-silver (ZnO-Ag), zinc oxide-copper (ZnO-Cu) nanocomposites were synthesized with pure eco-friendly dye extracted from Bridelia ferruginea, zinc acetate (Zn(CH 3 COO) 2 ) as host, copper acetate (Cu (CH 3 COO) 2 ), and silver nitrate (AgNO 3 ) as dopant precursors. Phytochemical screening of dyes showed high, presence of phenols and terpenoids. The nanomaterials were characterized by Fourier-transform infrared, X-ray diffraction, scanning electron microscopy with energy dispersive X-ray analysis, transmission electron microscopy and ultraviolet-visible spectroscopy. The results showed the capping agents in the dyes were responsible for reducing the bulk materials. The crystallinity of the nanomaterials were found to be 19.02, 18.98 and 18.90 nm for the ZnO, ZnO-Ag and ZnO-Cu nanoparticles respectively. The ZnO nanoparticles were flakelike in shape, whereas the Cu and Ag doped particles were spherical. An optical bandgap of 4.73 eV was recorded for the dye and 3.24 eV for the ZnO nanoparticles. This was narrowed to 3.18 and 3.13 eV by silver and copper dopant respectively. These results showed the nanoparticles as a potential agent for photovoltaics and other optical applications.

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Section: Phytochemical Analysis Of the Dyementioning

confidence: 99%

Green synthesis of zinc oxide nanoparticles and zinc oxide–silver, zinc oxide–copper nanocomposites using Bridelia ferruginea as biotemplate

et al. 2020

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“…E. intermedia shrubs are always green and called ma-huang, and are locally known as "oman." Ma-huang (Ephedra herba) is resultant from the aerial parts of E. sinica Stapf, E. intermedia Schrenk, E. equisetina Bunge, and E. distachya L. It has been utilized medicinally as a stimulant, diaphoretic, and antiasthmatic (6,7,8). It is a xerophytic shrub and grows in unfavorable soil and climatic conditions such as high temperature and high light (9).…”

Section: Introductionmentioning

confidence: 99%

Formulations, characterization, in vitro and ex vivo release of Ephedra extract from topical preparations using dialysis cellulose membrane and natural rabbit skin

Gul¹,

Jan²,

Ahmad³

et al. 2017

Dissolution Technol.

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Ephedra is herbal medicine used in the treatment of many diseases, especially asthma and urticaria. The aim of this study was to formulate topical formulations as a microemulsion, a gel and an ointment containing the Ephedra extracts of (E. Intermedia), collected from Balochistan, Pakistan and to evaluate their isolation, characterization and in vitro and ex vivo permeability release using markers. High performance liquid chromatography (HPLC) was used for the quantification of markers, and in vitro and ex vivo studies were done by Franz diffusion cells. The kinetics release and permeability was checked by cuprophan dialysis membrane and natural rabbit skin to justify their stability for topical use. Of the three formulations, release behavior of microemulsion and gel was almost the same, and the ointment showed slow release. The cellulose membrane showed faster release than the rabbit skin; this arrangement can be shown as microemulsion > gel > ointment. However, Korsmeyer-Peppas release kinetics for the three formulations were observed for the membranes. This study demonstrates that it is necessary to assess the impact of release and permeability patterns of different formulations. In vitro and ex vivo experiments can be utilized to develop formulations of traditional medicines.

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“…The peak of quasimolecular ions in the positive ion mode [M+H] + was detected at m/z 543. 13. This was evident in the secondary mass spectra (fragment ions at m/z 499.12, 417.08, and 345.04).…”

Section: Purity Analyses and Identification Of Potential Lox And Ldhmentioning

confidence: 83%

Development of a method to screen and isolate bioactive constituents from Stellera chamaejasme by ultrafiltration and liquid chromatography combined with semi‐preparative high‐performance liquid chromatography and high‐speed counter current chromatography

Hou

Xia

Liu

et al. 2019

J of Separation Science

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A simple and efficient method based on ultrafiltration with liquid chromatography and mass spectrometry was used for the rapid screening and identification of ligands in the extracts of Stellera chamaejasme. The bound ligands, i.e. daphnoretin, isopimpinellin, chamaechromone, neochamaejasmin A, and chamaejasmine (purity of 96.8, 90.75, 91.41, 93.98, and 98.91%, respectively), were separated by semi‐preparative high‐performance liquid chromatography combined with high‐speed counter‐current chromatography. To the best of our knowledge, this is the first study to report the detection of potent lipoxidase and lactate dehydrogenase inhibitors in Stellera chamaejasme extracts. The results demonstrate that our method of ultrafiltration with liquid chromatography and mass spectrometry combined with mixed chromatography can be used to screen and confirm the bioactivity of all isolated compounds. This method also eliminates the need for separation of inactive compounds, thereby improving efficiency when studying bioactive substances. For some complex mixtures, neither semi‐preparative high‐performance liquid chromatography nor high‐speed counter‐current chromatography can purify all the target active compounds with high purity in a one‐step separation. The combination of the two methods allow for efficient purification of target bioactive compounds with different polarities and physicochemical properties based on their complementary properties.

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Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan

Cited by 360 publications

References 18 publications

Green synthesis of zinc oxide nanoparticles and zinc oxide–silver, zinc oxide–copper nanocomposites using Bridelia ferruginea as biotemplate

Green synthesis of zinc oxide nanoparticles and zinc oxide–silver, zinc oxide–copper nanocomposites using Bridelia ferruginea as biotemplate

Formulations, characterization, in vitro and ex vivo release of Ephedra extract from topical preparations using dialysis cellulose membrane and natural rabbit skin

Development of a method to screen and isolate bioactive constituents from Stellera chamaejasme by ultrafiltration and liquid chromatography combined with semi‐preparative high‐performance liquid chromatography and high‐speed counter current chromatography

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