Preliminary Recognition of c-Myc Gene Protein Using an Optical Biosensor with Gold Colloid Nanoparticles Based on Localized Surface Plasmon Resonance

Abstract

The gold colloidal nanoparticles with a diameter of 24 AE 0.2 nm were prepared, characterized by ultraviolet-visible (UV-vis), transmission electron 2820 microscopy (TEM), and cyclic voltammetry, and assembled on a gold plate substrate for constructing an optical nanobiosensor. The nanosensor exhibited distinct optical properties of localized surface plasmon resonance (LSPR), which could be used for recognition of oncogene biomolecules such as the c-Myc (3C7) antibody protein. The LSPR nanobiosensor has also been successfully applied to determination of a pCMV-Myc mammalian expression vector, one kind of DNA plasmids with a linear response range of 6.2-20.0 ng=mL and a detection limit of 2.4 ng=mL.

Emerging use of nanostructure films containing capped gold nanoparticles in biosensors

“…Cao et al 100 have developed an optical nanobiosensor using 24 nm sGNP and successfully performed real time DNA detection. Monoclonal antibodies, c-Myc, bound nanosensor showed a linear response for pCMV-Myc plasmid detection ranging from 6.2 to 20.0 ng/μL concentration (R 2 = 0.99).…”

Investigation on Electronic Signals for Detection of Target DNA Molecule Based on Extended Gate FET Sensing Chip

“…DNA chips based on surface-confined DNA probe arrays have played an important role in molecular biology, pharmaceutical research, and clinical applications [1]. Although many researchers have employed with fluorescence detection for the DNA chips [2], various types of solid state biosensors have been developed by combining semiconductor technology and bio-nano technology in recent years [3][4][5]. Particularly, field-effect-transistor (FET) sensors have seen increased interests on electrochemical detection of DNA hybridization events on solid surfaces [5,6].…”