Preliminary report on isolation of mycobacteria from patients with Crohn's disease

Gitnick, Gary; Collins, Judith; Beaman, Blaine L.; Brooks, Dale L.; Arthur, Marika; Imaeda, T; Palieschesky, Mary

doi:10.1007/bf01540280

Cited by 86 publications

(43 citation statements)

References 34 publications

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“…Similar isolation of the organism from Crohn's disease patients was described by Chiodini (1989), Pavlík et al (1994), and Thompson (1994). M. paratuberculosis has also been cultured in the laboratory from humans with chronic inflammation of the intestine of the Crohn's disease type, but in very rare cases (Coloe et al, 1986;Gitnick et al, 1989;Thorel, 1989;Haagsma et al, 1991).…”

Section: A Possible Role In Crohn's Diseasementioning

confidence: 53%

The transmission and impact of paratuberculosis infection in domestic and wild ruminants

Ayele¹,

Macháčková²,

Pavlík³

2001

Vet. Med.

109

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Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) infects domestic cattle, sheep, goats, deer, camelids and wild ruminants leading to chronic enteritis known as paratuberculosis (Johne’s disease). The infection is chronic, progressive and unresponsive to treatment. Most infected animals do not develop clinical disease but may excrete the bacteria. Clinically sick animals suffer emaciation and in some species diarrhoea, followed by eventual death. During the course of the disease, excretion of M. paratuberculosis in faeces and milk occurs, and the organism spreads through the blood and lymph vessels of infected animals to multiple internal organs. The infection disseminates to both the female and male reproductive organs. Though M. paratuberculosis is not classified as a human pathogen, current opinions on the possible role of this mycobacteria in public health is discussed. This article attempts to review the ways and circumstances by which M. paratuberculosis is transmitted within an animal population and the importance of the disease on animal production. Published reports concerning the transmission and epidemiology of the disease are reviewed herein, and preventive and control measures are summarised.

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Section: A Possible Role In Crohn's Diseasementioning

confidence: 53%

The transmission and impact of paratuberculosis infection in domestic and wild ruminants

Ayele¹,

Macháčková²,

Pavlík³

2001

Vet. Med.

109

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“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20], and 314 mice euthanatized at 5 months (Nos. 2 1-23) appeared healthy.…”

Section: Clinical Featuresmentioning

confidence: 99%

Mycobacterium paratuberculosis Monoassociated Nude Mice as a Paratuberculosis Model

et al. 1991

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Abstract. In this study, a paratuberculosis (Johne's disease) model was developed by intragastrically dosing gnotobiotic athymic nude mice with Mycobacterium paratuberculosis. The mice infrequently shed bacilli from their intestinal tracts during the first 4 months after inoculation. Following this time, increasing numbers of M. paratuberculosis (greater than 4.0 log,, bacilli per fecal pellet by 40 weeks) were recovered from the feces of the 12 mice that remained in the isolator. A similar pattern of recovery of M. paratuberculosis was obtained from the ileum, cecum, colon, and liver. Histopathologic lesions and acid-fast bacilli were rare during the first 4 months of infection and then, with time, increased in prevalence and severity. Mice maintained for 7 months or longer exhibited severe granulomatous inflammation and large numbers of acid-fast bacilli in the gastrointestinal tract and liver (up to lo8 log,, colony forming units per gram wet weight). Five mice maintained for 7 months or more developed clinical signs consistent with those seen in paratuberculosis (weight loss, chronic diarrhea); three of these mice eventually died or became moribund and were euthanatized. M. paratuberculosis monoassociated mice released increased levels of tumor necrosis factor activity into their sera, as compared to uninfected control mice, when they were injected with bacterial lipopolysaccharide. The clinical signs, fecal shedding of M. paratuberculosis, granuloma formation, and progressive bacillary multiplication observed with these mice are consistent with naturally occurring M. paratuberculosis infection of ruminants (Johne's disease). This model will be useful for future studies of immunoregulation and antimicrobial therapy of paratuberculosis.

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“…In some studies, M. avium subsp. paratuberculosis was isolated from intestinal biopsies or feces of Crohn's disease patients (6,10,11,19,23). Furthermore, a recent study in the United Kingdom revealed a significant correlation between the presence of M. avium subsp.…”

mentioning

confidence: 99%

Use of Multilocus Variable-Number Tandem-Repeat Analysis for TypingMycobacterium aviumsubsp.paratuberculosis

Overduin

Schouls²,

Roholl

et al. 2004

J Clin Microbiol

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The etiology of Crohn's disease in humans is largely unknown. Clinical signs of Crohn's disease partly resemble the clinical picture of Johne's disease in ruminants caused by Mycobacterium avium subsp. paratuberculosis. Because of the high prevalence of these bacteria in (products of) ruminants and their remarkable thermostability, concern has been raised about the possible role of these bacteria in the pathogenesis of Crohn's disease. In an attempt to develop a molecular typing method to facilitate meaningful comparative DNA fingerprinting of M. avium subsp. paratuberculosis isolates from the human and animal reservoirs, multilocus variable-number tandem-repeat analysis (MLVA) was explored and compared to IS900 restriction fragment length polymorphism (RFLP) typing. MLVA typing subdivided the most predominant RFLP type, R01, into six subtypes and thus provides a promising molecular subtyping approach to study the diversity of M. avium subsp. paratuberculosis

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Preliminary report on isolation of mycobacteria from patients with Crohn's disease

Cited by 86 publications

References 34 publications

The transmission and impact of paratuberculosis infection in domestic and wild ruminants

The transmission and impact of paratuberculosis infection in domestic and wild ruminants

Mycobacterium paratuberculosis Monoassociated Nude Mice as a Paratuberculosis Model

Use of Multilocus Variable-Number Tandem-Repeat Analysis for TypingMycobacterium aviumsubsp.paratuberculosis

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