PRELIMINARY RESULTS ON ARTIFICIAL PROPAGATION OF ASIAN PAINTED FROG (Kaloula pulchra) USING LH-RHa

Abstract

This study on artificial propagation of the Asian painted frog (Kaloula pulchra) was conducted to evaluate the reproductive efficiency of LH-RHa stimulation. The experiment samples were randomized into 4 different dosage groups with LH-RHa, including 60, 80, 100 and 120µg/kg of body weight, and each trial was repeated four times. The results showed that the appropriate dose for Asian painted frog reproduction was the 60mg/kg of LH-RHa (treatment 1). Some reproductive parameters were documented on treatment 1 including: latencytime (5.2 ± 0.65 hours at 29.2 ± 0.170C), spawning rate (91.8 ± 8.25%), fecundity (47.866 ± 2.377 eggs per kg of female), fertilization rate (96.8 ± 0.63%), hatching time (16.2 ± 1.23 hours at 29.2 ± 0.17oC), hatch ing rate (96.6 ± 1.03%), and metamorphosis duration (14.5 ± 0.2 days at 30.5 ± 0.31oC). The average survival rate of the froglets in the treatment 1 group during 30 days of nursing was at 26.7 ± 1.33%. Moreover, this is one of the first preliminary studies about propagation of the Asian painted frog (Kaloula pulchra) in Vietnam.