Preliminary results on the postmortem measurement of 3-beta-hydroxybutyrate in liver homogenates

Palmière, Cristian; Mangin, Patrice; Werner, Dominique

doi:10.1007/s00414-013-0870-3

Cited by 5 publications

(5 citation statements)

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“…Beta-hydroxybutyrate concentrations in liver homogenates correlated well with blood values in both diabetic and non-diabetic patients and were not influenced by time after death, thus allowing diabetic ketoacidosis to be diagnosed. Analogously, blood and synovial fluid beta-hydroxybutyrate concentrations were correlated with increased blood levels reflected in increased synovial fluid concentrations ( 76 , 77 ).…”

Section: Acetone Acetoacetate and Beta-hydroxybutyratementioning

confidence: 90%

“…Interesting results were obtained by Palmiere et al ( 76 , 77 ), who measured beta-hydroxybutyrate levels in liver homogenates and synovial fluid. Beta-hydroxybutyrate concentrations in liver homogenates correlated well with blood values in both diabetic and non-diabetic patients and were not influenced by time after death, thus allowing diabetic ketoacidosis to be diagnosed.…”

Section: Acetone Acetoacetate and Beta-hydroxybutyratementioning

confidence: 96%

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Postmortem diagnosis of diabetes mellitus and its complications

Palmière¹

2015

Croat Med J

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Diabetes mellitus has become a major cause of death worldwide and diabetic ketoacidosis is the most common cause of death in children and adolescents with type 1 diabetes. Acute complications of diabetes mellitus as causes of death may be difficult to diagnose due to missing characteristic macroscopic and microscopic findings. Biochemical analyses, including vitreous glucose, blood (or alternative specimen) beta-hydroxybutyrate, and blood glycated hemoglobin determination, may complement postmortem investigations and provide useful information for determining the cause of death even in corpses with advanced decompositional changes. In this article, we performed a review of the literature pertaining to the diagnostic performance of classical and novel biochemical parameters that may be used in the forensic casework to identify disorders in glucose metabolism. We also present a review focusing on the usefulness of traditional and alternative specimens that can be sampled and subsequently analyzed to diagnose acute complications of diabetes mellitus as causes of death.

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Section: Acetone Acetoacetate and Beta-hydroxybutyratementioning

confidence: 90%

Section: Acetone Acetoacetate and Beta-hydroxybutyratementioning

confidence: 96%

Postmortem diagnosis of diabetes mellitus and its complications

Palmière¹

2015

Croat Med J

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“…Furthermore, blood β-hydroxybutyrate levels in decomposed bodies can be considered an appropriate biochemical parameter in the estimation of β-hydroxybutyrate concentrations at the time of death. Indeed, β-hydroxybutyrate concentration does not increase after death and may, at most, decrease as a result of spontaneous molecule degradation [41][42][43].…”

Section: Postmortem Biochemistrymentioning

confidence: 99%

“…Recent studies have indicated that β-hydroxybutyrate can be reliably measured in liver homogenates and may prove useful in confirming the presence of metabolic disturbances at the time of death. Liver homogenates can therefore be considered an alternative biological sample for βhydroxybutyrate determination; limited amounts of biological fluids must be reserved for toxicology and biochemical analyses [43].…”

Section: Postmortem Biochemistrymentioning

confidence: 99%

Postmortem diagnosis of hypothermia

2014

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The identification of hypothermia as the cause of death has always been somewhat problematic in forensic pathology because of unspecific, inconstant, or even negative macroscopic and microscopic findings. Though the simultaneous presence of frost erythema, Wischnewski spots, hemorrhages into the synovial membrane, bloody discoloration of synovial fluid of the knee, and basal vacuolization of the renal tubular epithelial cells has been indicated as strongly supportive of fatal hypothermia, their absence does not allow the diagnosis of hypothermia to be ruled out. Postmortem biochemical investigations are valuable in detecting adaptation responses to cold stress and metabolic changes that occur following cold exposure. However, ethanol intoxication prevents appearance of adaptation responses to cold, rendering the diagnosis less obvious. Immunohistochemistry, postmortem imaging, and molecular pathology have shown promising results, although at present, they do not provide pathognomonic signs of fatal hypothermia. The aim of this article is to present a review of the literature covering the significance of different postmortem investigations that are associated with hypothermia fatalities.

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“…Within a long postmortem delay and thus taphonomic processes, there is a disappearance of blood, vitreous humor and pericardial fluid though the liver persists. In this context, Palmiere et al [52] published preliminary but promising results in 2013 regarding the exploration of HB in "liver homogenates". In the future, pathologies with hyperketonemia, in our case hypothermia, could be explored despite long postmortem intervals.…”

Section: Ketones Bodiesmentioning

confidence: 99%

Updated review of postmortem biochemical exploration of hypothermia with a presentation of standard strategy of sampling and analyses

Rousseau

Reynier

Jousset

et al. 2018

Clinical Chemistry and Laboratory Medicine (CCLM)

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Hypothermia is defined as a core body temperature below 35°C and can be caused by environmental exposure, drug intoxication, metabolic or nervous system dysfunction. This lethal pathology with medico-legal implications is complex to diagnose because macroscopic and microscopic lesions observed at the autopsy and the histological analysis are suggestive but not pathognomonic. Postmortem biochemical explorations have been progressively developed through the study of several biomarkers to improve the diagnosis decision cluster. Here, we present an updated review with novel biomarkers (such as catecholamines O-methylated metabolites, thrombomodulin and the cardiac oxyhemoglobin ratio) as well as some propositional interpretative postmortem thresholds and, to the best of our knowledge, for the first time, we present the most adapted strategy of sampling and analyses to identify biomarkers of hypothermia. For our consideration, the most relevant identified biomarkers are urinary catecholamines and their O-methylated metabolites, urinary free cortisol, blood cortisol, as well as blood, vitreous humor and pericardial fluid for ketone bodies and blood free fatty acids. These biomarkers are increased in response either to cold-mediated stress or to bioenergetics ketogenesis crisis and significantly contribute to the diagnosis by exclusion of death by hypothermia.

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Preliminary results on the postmortem measurement of 3-beta-hydroxybutyrate in liver homogenates

Cited by 5 publications

References 50 publications

Postmortem diagnosis of diabetes mellitus and its complications

Postmortem diagnosis of diabetes mellitus and its complications

Postmortem diagnosis of hypothermia

Updated review of postmortem biochemical exploration of hypothermia with a presentation of standard strategy of sampling and analyses

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