Preliminary screening of anti-inflammatory effect of phytochemicals on chemotaxis of human neutrophils

Abstract

Neutrophils are leukocytes that are actively recruited to sites of tissue infection and/or injury by directed movement (chemotaxis).In vitro assessment of inhibition of neutrophil chemotaxis is a physiologic indicator of anti-inflammatory potential. To identify nontoxic, anti-inflammatory agents, plant-derived compounds (curcumin, resveratrol, rosmarinic acid and piperine) were assessed for effects on in vitro neutrophil movement. Effects were determined on directed migration (chemotaxis) towards the potent chemoattractant of bacterial cell wall origin, f-met-leu-phe (fMLP). Curcumin significantly inhibited neutrophil chemotaxis in a concentration-dependent manner with statistically significant inhibition at 50 and 100 μM. Similarly, resveratrol (25, 50, 100 μM) and rosmarinic acid (100 μM) significantly inhibited fMLP-induced chemotaxis in concentration-dependent manners. Piperine had no effect on neutrophil chemotaxis. These results indicate that curcumin, resveratrol and rosmarinic acid have the potential to elicit anti-inflammatory effects.

The potential role of some phytochemicals in recognition of mitochondrial damage-associated molecular patterns

“…Other authors demonstrated the inhibition of LPS-induced neutrophil invasion in air pouches model in mice, as well as the leukocyte chemotaxis towards fMLF in vitro (Antoine et al, 2013;Srivastava, 1989;Uthayashanker and Rita, 2015). These observations suggest some involvement of inhibition of FRP signaling by this dietary agent, although it has not been investigated yet, if the FPR is directly a molecular target of CMN.…”

Effect of Ylang-Ylang (Cananga odorata Hook. F. & Thomson) Essential Oil on Acute Inflammatory Response In Vitro and In Vivo

“…fMLP is a chemoattractant that induces signal transduction events, leading to several cellular processes, including the diapedesis, chemotaxis, and migration of polymorphonuclear leukocytes (PMN) [31]. Chemotaxis is induced through the production of several pro-inflammatory cytokines, such as interleukin-1β (IL-1β), IL-8, and tumor necrosis factor (TNF), through mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI-3K) activation, crucial cascades in the development of functional responses and neutrophils in inflammation [32,33]. Linalool, one constituent of YEO, attenuated the production of TNF and IL-6 in cell culture stimulated by lypopolisaccharide (LPS) [34].…”

Chapter 1. Foodomics – Fundamentals, State of the Art and Future Trends

“…CE has demonstrated to be a very useful analytical tool in food science 207 , but only a few studies have coupled it to MS for the identification of bioactive metabolites 157,158 . On the other hand, the use of GC-MS is more widespread, and it has been used to identify and characterize possible bioactive compounds in extracts from mint seeds 159 , nettle leaves 160 , ginseng roots 161 , pistachio resin 162,163 , or Nigella sativa seeds 164 . But on top of these technologies, the gold standard method of choice is LC-MS. As it can be observed in Table X.2, more than 30 studies have been published on this topic over the last 10 years, and more than half in the last 2 years 165,194 .…”