Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability

Abstract

Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes were increased in all strains in the presence of Cu2+. Strain B showed the greatest response to Cu2+ addition, whereas strain A was more stable at the optimal temperature and pH. Strain A showed interesting potential for future biotechnological approaches due to the superior thermo-stability of its secreted enzymes.

Endophytic fungal community structure in olive orchards with high and low incidence of olive anthracnose

“…The capacity of non-pathogenic strains of F. oxysporum to control several fungal diseases has been also demonstrated 35 . Species of the genus Trametes are well known for their ability to produce enzymes, which could assist in the defense of the plant against pathogens 36 . Similarly, species from Pezizomycetes have been reported to offer benefits to their host plants 37 .…”

Discovery of novel xylosides in co-culture of basidiomycetes Trametes versicolor and Ganoderma applanatum by integrated metabolomics and bioinformatics

“…5.249, Fructificatio Amaurodermatis Rudae, Ganoderma lucidum, Pleurotus nebrodensis, Phellinus igniarius CGMCC No.8108 , Cordyceps militaris, Boletus edulis, Trametes versicolor CGMCC No.12241 , Trametes robiniophia, Schizophyllum commune, Sclerotium Xylariae Nigripis ) were deposited at the Shandong Province Key Lab of Applied Mycology, China. The fungus was identified based on analysis of internal transcribed spacer sequences 39 40 . The culture medium consisted of glucose (2 g), KH 2 PO 4 (0.2 g), MgSO 4 (0.1 g), and peptone (0.4 g), and agar (4 g, only in solid medium) in 200 mL of sterilized water.…”

Research Progress on Microbial Sources of Laccase and its Applications in Food Industry

“…Fonseca and others evaluated the secretion ability of laccase produced by four newly discovered strains Trametes sp. and found that the laccase secreted by one of the strains was highly thermostable and showed its potential in the development of biotechnology [16]. Chen Qionghua [17] and others used Ganoderma weberianum TZC-1 as a production strain and tried to enlarge the strain in a 50L fermentor under the conditions of optimized shake flask culture and a small test production process on a 5L fermentor.…”