Preliminary Studies on the Analgesic Properties of the Ethanol Extract of Combretum leprosum

Lira, Silvéria Regina de Sousa; Almeida, Reinaldo Nóbrega de; Almeida, Fernanda Campos Sousa de; Oliveira, Fernando de Sousa; Duarte, José Crispim

doi:10.1076/phbi.40.3.213.5837

Cited by 21 publications

(5 citation statements)

References 6 publications

(6 reference statements)

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“…The lower 3 cm portion of the tail of each mouse was immersed in a hot water beaker kept at 50 ± 0.5 °C, and the time of tail withdrawal (or reaction time) was recorded in seconds, at (0 min) and after 15, 30, 45, 60 and 75 min of the administration. The maximum time of each immersion did not exceed 15 seconds to prevent thermal injury to animals [1 7 ].…”

Section: Methodsmentioning

confidence: 99%

Prospecting for candidate molecules from Conus virgo toxins to develop new biopharmaceuticals

Mohamed

Nabil

El-Naggar

2022

J. Venom. Anim. Toxins incl. Trop. Dis

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Background A combination of pharmacological and biomedical assays was applied in this study to examine the bioactivity of Conus virgo crude venom in order to determine the potential pharmacological benefit of this venom, and its in vivo mechanism of action. Methods Two doses (1/5 and 1/10 of LC 50 , 9.14 and 4.57 mg/kg) of the venom were used in pharmacological assays (central and peripheral analgesic, anti-inflammatory and antipyretic), while 1/2 of LC 50 (22.85 mg/kg) was used in cytotoxic assays on experimental animals at different time intervals, and then compared with control and reference drug groups. Results The tail immersion time was significantly increased in venom-treated mice compared with the control group. Also, a significant reduction in writhing movement was recorded after injection of both venom doses compared with the control group. In addition, only the high venom concentration has a mild anti-inflammatory effect at the late inflammation stage. The induced pyrexia was also decreased significantly after treatment with both venom doses. On the other hand, significant increases were observed in lipid peroxidation (after 4 hours) and reduced glutathione contents and glutathione peroxidase activity, while contents of lipid peroxidation and nitric oxide (after 24 hours) and catalase activity were depleted significantly after venom administration. Conclusion These results indicated that the crude venom of Conus virgo probably contain bioactive components that have pharmacological activities with low cytotoxic effects. Therefore, it may comprise a potential lead compound for the development of drugs that would control pain and pyrexia.

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Section: Methodsmentioning

confidence: 99%

Prospecting for candidate molecules from Conus virgo toxins to develop new biopharmaceuticals

Mohamed

Nabil

El-Naggar

2022

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Combretum leprosum (Figure 1a-c) is a shrubby plant found in the Northeast region of Brazil, popularly known as "mufumbo," "mofumbo," or "cipoaba" [30] and is highly prevalent in the state of Cear a. In incidence, it is considered the third most preponderant species of the Cear a flora [31].…”

Section: Ethnopharmacology and Phytochemical Studymentioning

confidence: 99%

Pharmacological potential of the triterpene 3β,6β,16β‐trihidroxilup‐20 (29)‐ene isolated from Combretum leprosum: A literature review

Coutinho

Oliveira

Evaristo

et al. 2022

Fundamemntal Clinical Pharma

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Globally, plant‐derived medicines have been playing an increasing and relevant role in the treatment of several diseases, thus fostering the search for new bioactive substances. Among the various families of plants studied, those of the Combretum genus can be highlighted since they are widely used in folk medicine for the treatment of hepatitis, malaria, respiratory infections, cancer, skin hemorrhage, and anxiety. Phytochemical studies carried out on species of the Combretum genus demonstrated the presence of several classes of bioactive chemical compounds, including the triterpene 3β,6β,16β‐trihydroxilup‐20(29)‐ene (CLF‐1). In this perspective, the objective of this review was to gather all pharmacological activities attributed to the CLF‐1 triterpene, highlighting its importance for the pharmaceutical industry. The research was performed in scientific databases such as PubMed, SciELO, LILACS, SciFinder and Science Direct. The literature indicates a great pharmacological potential of CLF‐1, evidencing its antioxidant, anti‐inflammatory, antiviral, antiparasitic, antinociceptive, healing, and antibacterial action, antinociceptive and antitumor effect. Therefore, based on the different research above, it is plausible to consider CLF‐1, obtained from different parts of the C. leprosum plant, as a molecule with biotechnological potential that may contribute to the development of new drugs and, consequently, in the treatment of various human pathologies.

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Section: Central Analgesic Assay (Tail Immersion Test)mentioning

confidence: 99%

Recombinant expression and antigenicity of two peptide families of neurotoxins from Androctonus sp.

Cardoso-Arenas

Clement

Arenas

et al. 2022

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: A combination of pharmacological and biomedical assays was applied in this study to examine the bioactivity of Conus virgo crude venom in order to determine the potential pharmacological benefit of this venom, and its in vivo mechanism of action. Methods: Two doses (1/5 and 1/10 of LC 50 , 9.14 and 4.57 mg/kg) of the venom were used in pharmacological assays (central and peripheral analgesic, anti-inflammatory and antipyretic), while 1/2 of LC 50 (22.85 mg/kg) was used in cytotoxic assays on experimental animals at different time intervals, and then compared with control and reference drug groups. Results: The tail immersion time was significantly increased in venom-treated mice compared with the control group. Also, a significant reduction in writhing movement was recorded after injection of both venom doses compared with the control group. In addition, only the high venom concentration has a mild anti-inflammatory effect at the late inflammation stage. The induced pyrexia was also decreased significantly after treatment with both venom doses. On the other hand, significant increases were observed in lipid peroxidation (after 4 hours) and reduced glutathione contents and glutathione peroxidase activity, while contents of lipid peroxidation and nitric oxide (after 24 hours) and catalase activity were depleted significantly after venom administration. Conclusion: These results indicated that the crude venom of Conus virgo probably contain bioactive components that have pharmacological activities with low cytotoxic effects. Therefore, it may comprise a potential lead compound for the development of drugs that would control pain and pyrexia.

show abstract

Preliminary Studies on the Analgesic Properties of the Ethanol Extract of Combretum leprosum

Cited by 21 publications

References 6 publications

Prospecting for candidate molecules from Conus virgo toxins to develop new biopharmaceuticals

Prospecting for candidate molecules from Conus virgo toxins to develop new biopharmaceuticals

Pharmacological potential of the triterpene 3β,6β,16β‐trihidroxilup‐20 (29)‐ene isolated from Combretum leprosum: A literature review

Recombinant expression and antigenicity of two peptide families of neurotoxins from Androctonus sp.

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