Preliminary study of factors affecting the superovulatory response of high producing dairy cows superstimulated regardless of the stage of estrous cycle in Egypt

Hussein, M. M.; Aziz, R.L. Abdel; Abdel‐Wahab, Ahmed; El-Said, H.

doi:10.1016/j.bjbas.2014.11.002

Cited by 4 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This variation might indicate the presence of a more important individual variation rather than breed influence when it comes to superovulatory treatment in bovine (Curtis, 2015). The average CL detection was comparable to previous studies for the HF crossbreeds (Ferreira et al, 2014;Vieira et al, 2014;Tadesse et al, 2016) though fewer numbers have also been reported (Hussein et al, 2014). Several other studies (Peixoto et al, 2007;Vieira et al, 2014;Mapletoft et al, 2015;Perez et al, 2016;Perez et al, 2019;Vizoná et Ethiop.…”

Section: Discussionsupporting

confidence: 85%

Influence of breed on the quality of in vivo produced embryos from Boran and Holstein Friesian cross dairy breed in Ethiopia

Jemal¹,

Degefa²,

Ali³

et al. 2021

Ethiop. Vet. J.

View full text Add to dashboard Cite

The variation of the dairy breed can determine the success of bovine embryo transfer by influencing the quantity and quality of in vivo embryo production. In this experiment, output and quality of in vivo produced embryos using semen of progeny tested Holstein Friesian (HF) sire in Boran and HF*Boran F1 cross cows, and semen from purebred Boran sire in HF*Boran F1 cross and Boran cows were evaluated. Boran (n=18) and HF*Boran cross (n=18) breed donor dams were superovulated using a previously optimized follicular stimulating hormone (FSH) (Pluset®) dose regimen: 650 IU for HF*Boran cross and 250 IU for Boran breeds. Each cow was flushed on Day-7 post insemination and embryos were evaluated for their developmental stages and quality. Superovulatory response rates were 88.9% and 83.3%, respectively, for Boran and HF*Boran with no significant (P>0.05) breed differences. Total recovery rates were relatively lower (56.5%) in Boran compared to in HF*Boran (67.4%). The mean (±SE) embryo flush outputs were 6.5±0.8 for Boran and 6.9±0.7 forHF*Boran with no significant breed difference. Recovery of a transferrable embryo was significantly higher (68.0%; P<0.05) in HF*Boran dam inseminated with HF sire semen. Boran cows yielded a significantly higher (P<0.05) proportion of unfertilized ovum (57.6 %) irrespective of the sire breeds. Comparatively, a higher number of degenerated embryos were produced by HF*Boran cows. This study demonstrated that the presence of breed-related differences in both the quality and quantity of in vivo produced Bovine embryos.

show abstract

Section: Discussionsupporting

confidence: 85%

Influence of breed on the quality of in vivo produced embryos from Boran and Holstein Friesian cross dairy breed in Ethiopia

Jemal¹,

Degefa²,

Ali³

et al. 2021

Ethiop. Vet. J.

View full text Add to dashboard Cite

show abstract

“…Betterridge (1997) reported that the unusual endocrine conditions after superovulation adversely affect embryo recovery and viability, and embryo recovery rates would decrease over time after ovulation. The embryo recovery rates achieved in the present study were lower than those reported in some other studies (Bülbül et al 2010;Akyol et al 2014;Hussein et al 2014;Karaşahin et al 2016). Kanagawa et al (1995) reported that the differences in embryo recovery rates between studies were related to factors such as washing time, washing media and washing number, catheter type and positioning of the catheter, technician experiences, etc.…”

Section: Discussioncontrasting

confidence: 79%

“…Furthermore, after superovulation using FSH, Köse et al (2007) reported the numbers of Grade 1 and Grade 2 embryos as 1.9±0.7 and 1.9±0.7, respectively, and indicated the number of transferable embryos as 4.1±1.0. After applying superovulation treatment with CIDR+FSH, Hussein et al (2014) reported the mean number of transferable embryos as 4.86±0.57 (68.46%), and the mean numbers of Grade 1, Grade 2 and degenerate embryos as 3.68±0.50 (50.54%), 0.90±0.30 (14.31%) and 2.33±0.40 (31.36%), respectively. In the present study, the percentages of Grade 1, Grade 2, degenerate and transferable embryos were 66.7, 22.2, 11.1 and 88.9%, respectively (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Embryo Transfer in Fleckvieh Simmental Cows: A Preliminary Study

2022

Int J Vet Sci

View full text Add to dashboard Cite

This study was aimed at investigating pregnancy rates achieved with multiple ovulation and embryo transfer in Simmental cows, which are characterized by a high milk yield. For this purpose, eight cows were used as donors and for each donor, recipient groups of 10 animals, including both heifers and cows, were established. Recipients were synchronized by two intramuscular injections of prostaglandin F2α 11 days apart. The treatment of the donors consisted of the intravaginal placing of a CIDR on day 0, the application of 400mg FSH in decreasing doses, starting on day four, the administration of prostaglandin F2α and the removal of CIDR on day 8. The donors were inseminated twice on day 9 and once on day 10. Uterine flushing was performed, trans-cervically, on day 7 after insemination. The ovulation rate was determined as 12.25±1.42. Sixteen transferable embryos (2.0±3.23, 88.9%) were collected. The 12 embryos of Grade 1 quality and the 4 embryos of Grade 2 quality were transferred by transcervical route to 12 heifers and 4 cows, respectively. The pregnancy rates achieved in the heifers and cows were 50% and 25%, respectively (P>0.05). As a result, the MOET scheme applied in Fleckvieh Simmental Cows produced satisfactory superovulatory responses and resulted in the collection of quality embryos. The embryo recovery rate was poor but, overall, the ovulation rate, the quality of the collected embryos and the pregnancy rates achieved under field conditions in this preliminary study were considered to be satisfactory.

show abstract

“…Tingkat respon dihitung dengan membandingkan jumlah ternak yang memberikan respon superovulasi terhadap total ternak yang diberikan superovulasi. Sapi yang disuperovulasi dianggap memberikan respon apabila terdapat CL lebih dari sama dengan 3 (Hussein et al, 2014).…”

Section: Tingkat Respon (Respon Rate)unclassified

“…Diduga hal tersebut yang menyebabkan respon superovulasi pada seluruh kelompok memberikan hasil yang tidak berbeda. Hussein et al (2014) menyatakan bahwa respon ternak donor terhadap perlakuan superovulasi tergantung pada ras, umur dan kondisi umum, jenis hormon, iklim, nutrisi dan lingkungan.…”

Section: Jumlahunclassified

Respon Superovulasi Sapi Persilangan Belgian Blue dengan Metode yang Berbeda

Darlian

Wahjuningsih

Rosmayanti³

et al. 2021

J. Agripet

View full text Add to dashboard Cite

ABSTRACT. Superovulasi merupakan suatu metode pemanfaatan induk betina unggul untuk menghasilkan embrio sebanyak-banyaknya untuk kegiatan transfer embrio. Tujuan penelitian ini membandingkan 2 teknik superovulasi yang berbeda pada sapi persilangan Belgian Blue. Penelitian dilakukan di Balai Embrio Ternak Cipelang Bogor dengan menggunakan 24 ekor sapi persilangan Belgian Blue dengan umur 2-3 tahun, masing-masing perlakuan 12 ekor. Penelitian dilakukan secara eksperimen dengan dua perlakuan, P1 = Superovulasi menggunakan penyuntikan FSH selama 3 hari pada pagi dan sore (metode konvensional) dengan dosis 400 mg FSH dalam 20 ml pelarut dan P2 = Superovulasi menggunakan penyuntikan tunggal (satu kali) FSH dengan dosis 400 mg FSH dalam 3 ml pelarut . Parameter pada penelitian ini adalah Jumlah Corpus luteum (CL), respon rate, perolehan embrio, kualitas embrio, recovery rate, dan tingkat fertilisasi. Data yang diperoleh diuji dengan uji T tidak berpasangan. Hasil analisa data menunjukkan hasil yang tidak berbeda nyata (P0,05) pada jumlah CL P1 : 8,42+3,06 dan P2 : 6,08+4,74; Respon rate P1 : 100% dan P2 : 75%; Total perolehan embrio P1 : 6,08+2,64 embrio dan P2 : 4,45+4,01 embrio; Embrio recovery rate P1 : 70,37+9,18% dan P2 : 61,33+12,12%; Embrio Layak Transfer P1 : 3,83+2,92 embrio dan P2 : 2,73+2,28 embrio; dan berbeda nyata (P0,05) pada rataan fertilisasi P1 : 79,10% dan P2 : 95,26%. Kesimpulan penelitian ini adalah teknik superovulasi penyuntikan tunggal FSH secara subkutan memberikan efek superovulasi dan menghasilkan embrio dengan jumlah dan kualitas yang tidak berbeda nyata dengan teknik superovulasi konvensional. (Superovulation Responses of Belgian Blue Crossbreed Cattle Treated with Different Superovulation Methods) ABSTRAK. Superovulation is a technique for producing a large number of embryos for embryo transfer using a genetically superior female. The purpose of this research was to compare two alternative methods of superovulation in Belgian Blue crossbreed cattle. The study used 24 Belgian Blue crossbred cattle aged 2-3 years, including 12 cows per treatment, at the National Livestock Embryo Center of Cipelang in Bogor. The research was done in an experimental setting using two different treatments, P1 = Superovulation using twice daily FSH injections for three days at a dose of 400 mg FSH dissolved in 20 ml of saline, and P2 = Superovulation using FSH single injections at a dose of 400 mg FSH dissolved in 3 ml of saline. The parameters in this study were the number of corpus luteum (CL), response rate, total number of embryo/ova collection, embryo quality, recovery rate, and fertilization rate. The data obtained were tested by unpaired T test. The results of data analysis showed results that were not significantly different (P0.05) on the number of CL P1 : 8.42+3.06 and P2 : 6.08+4.74; Response rate P1 : 100% and P2 : 75%; Total number of embryos collection P1 : 6.08+2.64 embryos and P2 : 4.45+4.01 embryos; Embryo recovery rate P1 : 70.37+9.18% and P2 : 61.33+12.12%; Transferable Embryos P1 : 3.83+2.92 embryos and P2 : 2.73+2.28 embryos; and significantly different (P0.05) on fertilization rate P1 : 79.10% and P2 : 95.26%. The conclusion of this study show that the subcutaneous FSH single injection technique induces superovulation and produces embryos that are similar in number and quality to those produced by conventional superovulation techniques.

show abstract

Preliminary study of factors affecting the superovulatory response of high producing dairy cows superstimulated regardless of the stage of estrous cycle in Egypt

Cited by 4 publications

References 31 publications

Influence of breed on the quality of in vivo produced embryos from Boran and Holstein Friesian cross dairy breed in Ethiopia

Influence of breed on the quality of in vivo produced embryos from Boran and Holstein Friesian cross dairy breed in Ethiopia

Embryo Transfer in Fleckvieh Simmental Cows: A Preliminary Study

Respon Superovulasi Sapi Persilangan Belgian Blue dengan Metode yang Berbeda

Contact Info

Product

Resources

About