Preliminary study of L‐lysine production by Bacillus species using various agricultural by‐products

Abstract

The production of lysine by Bacillus megaterium SP-14 and Bacillus circulans Tx-22 using agricultural by-products as carbon and nitrogen sources was assessed. Among the carbon substrates used were potato, sorghum, plantain, millet, yam, cassava, and corn starches, while the nitrogen sources include cowpea, bambara-nut, cotton seed, groundnut, soybean, and blood meals. The effect of natural nitrogen sources (1.0% w/v) and synthetic nitrogen source (4.0% w/v (NH4)2SO4) on lysine production by the Bacillus strains showed that natural nitrogen sources gave better lysine yields.

Studies on lysine production by Bacillus megaterium

“…It cannot be synthesized internally but may be added to food and feed materials to improve the protein quality (Stillings et al, 1971). However, fermentative methods seem to be most economical and practicable means of producing lysine, and many of such processes have been investigated (Schrumpf et al, 1992;Eggeling, 1994;Ekwealor and Orafu, 2003). The present report is on studies carried out to maximize yields of free lysine produced in a culture broth by a bacterial strain recovered from soil.…”

Lysine Production of <i>Microbacterium lacticum</i> by Submerged Fermentation Using Various Hydrocarbon, Sugar and Nitrogen Sources

“…Sen and Chatterjee [37] isolated Arthrobacter globiform is that is capable of accumulating L-lysine in purely synthetic medium [37]. This is contrary to the work of Ekwealor and Orafu, (2003), who also noted that Bacillus sp. accumulated lysine in broth culture [10].…”

“…Lysine production processes by microorganism have been investigated by many researchers [8]- [10]. L-lysine can be produced chemically, enzymatically and biochemically just like other amino acids.…”

Studies on Lysine Accumulation in the Broth Culture of Bacillus Species using Carbohydrates as Carbon Sources and Seed Meals as Nitrogen Sources

“…Therefore cheap solid substrate i.e. Soybean meal was used for the maximum production of L-lysine as reported by Ekwealor and Orafu (2003), because it is a good source of proteinaceous nitrogen and other nutritious substances essential for bacterial growth. Umerie et al(2000) studied the effects of various nitrogen sources and was able to observe that defatted soyabean meal stimulated the highest amount of lysine.…”

“…Agro-industrial by -products are being used as nitrogen and carbon source in lysine production (Ekwealor and Ebele , 2003).Sugar cane molasses is a cheap carbon source, containing sucrose, glucose and fructose at a total carbohydrate content of 50 to 60% (Reed, 1982). Glucose, fructose and sucrose are important carbon sources that have a pronounced effect on kinetics and stoichiometry of lysine production by C. glutamicum (Kiefer et al,2002).…”