Preliminary study on diffraction enhanced radiographic imaging for a canine model of cartilage damage

Muehleman, Carol; Li, J.; Zhong, Z.

doi:10.1016/j.joca.2006.02.011

Cited by 23 publications

(8 citation statements)

References 27 publications

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“…The capacity of DEI to simultaneously visualize soft and mineralized tissues has obvious applications in the area of joint disease [10][11][12][13][14][15] and efforts are underway at BMIT to develop animal models of arthritic disease that will capitalize on this capability. DEI has also proven effective for bone imaging [16] and it has been frequently reported that DEI produces unique contrast visualization of trabecular bone architecture [17][18][19][20][21][22].…”

Section: Application Of Dei To Musculoskeletal Tissuesmentioning

confidence: 99%

Preliminary Bone Imaging on 05B1-1 Beamline at the Canadian Light Source: Exploration of Diffraction Enhanced Imaging

Cooper

Bewer

Wiebe

et al. 2011

Synchrotron Radiation News

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The bending magnet line (05B1-1) of the BioMedical Imaging and Therapy (BMIT) facility of the Canadian Light Source saw initial research application ( Figure 1) during commissioning which began in December 2008 and continued intermittently through May 2010. Following an initial run based upon letters of intent (June-Dec. 2010), the bending magnet line is now available for regular user access. The mandate to advance research related to human and animal health, combined with an active bone imaging community at the University of Saskatchewan, has resulted in a strong representation of skeletal imaging during initial experiments at BMIT. To date, specific manifestations of bone imaging have ranged from micro-CT imaging of human cortical bone samples to in vitro diffraction enhanced imaging (DEI) of rat and pig bones and, ultimately, to in vivo DEI of bones within young chickens. In this article we focus on the diffraction enhanced imaging capabilities of BMIT and describe our efforts to apply this technology to human trabecular bone microarchitecture -a potential clinical application in the future. Bone fractures and clinical assessmentBone fractures are related to compromised strength -a parameter which reflects both the quantity and quality of bone tissue [1]. While "quality" is a difficult term to define, most attempts focus on architectural properties. Bone architecture takes two general formsthe outer compact or cortical bone and the more lattice-like internal trabecular (spongy) bone. The current standard for clinical bone assessment, dual energy X-ray absorptiometry (DXA), provides only a 2D areal measurement of mineral density and no architectural information [2]. Advances in bone imaging, which provide improved architectural information, are currently being sought in the application of established clinical technologies (Quantitative Computed Tomography (QCT); magnetic resonance imaging (MRI)) and the development of new modalities such as High Resolution peripheral Quantitative Computed Tomography (HR-pQCT) [2,3]. Shifting from 2D to 3D methodologies has been a key focus, with the quantitative assessment of 3D trabecular architecture a common goal. While these pursuits are certainly bearing fruit, they also have practical limitations including, in the case of X-ray based approaches (e.g. QCT), increased dose relative to DXA [4][5][6]. The push for higher resolution (e.g. to visualize individual trabeculae) requires increased dose and this, in part, explains why even cutting-edge tools (e.g. HR-pQCT) are limited to around 100 um voxel size. Peripheral scanners, which target the limbs, have an additional limitation in that fracture sites located centrally within the body, such as the hip and vertebrae, cannot be imaged. It is within this larger context that we are exploring 2D projection DEI as a means of improving the assessment of trabecular bone microarchitecture with reduced radiation dose. Figure 1: Dean Chapman (left), Tomasz Wysokinski (middle), and Bill Thomlinson (right) prepare a sample in December 20...

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Section: Application Of Dei To Musculoskeletal Tissuesmentioning

confidence: 99%

Preliminary Bone Imaging on 05B1-1 Beamline at the Canadian Light Source: Exploration of Diffraction Enhanced Imaging

Cooper

Bewer

Wiebe

et al. 2011

Synchrotron Radiation News

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“…10 In-line phase contrast imaging (PCI) 11 is one SR X-ray imaging method based on similar, but simpler than DEI, principles and, thus, offers easier imaging modality. Due to its simplicity and ability to visualize microstructural details in various biological tissues and TE scaffolds, 12,13 PCI has been commonly used in TE.…”

Section: Introductionmentioning

confidence: 99%

Computed Tomography Diffraction-Enhanced Imaging forIn SituVisualization of Tissue Scaffolds Implanted in Cartilage

IzadifarZohreh

Dean²,

Chen³

2014

Tissue Engineering Part C: Methods

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Long-term in vivo studies on animal models and advances from animal to human studies should rely on noninvasive monitoring methods. Synchrotron radiation (SR)-diffraction enhanced imaging (DEI) has shown great promise as a noninvasive method for visualizing native and/or engineered tissues and bio-microstructures with appreciable details in situ. The objective of this study was to investigate SR-DEI for in situ visualization and characterization of tissue-engineered scaffolds implanted in cartilage. A piglet stifle joint implanted with an engineered scaffold made from poly-ɛ-caprolactone was imaged using SR computed tomography (CT)-DEI at an X-ray energy of 40 keV. For comparison, in situ visualization was also conducted with commonly used SR CT-phase contrast imaging and clinical magnetic resonance imaging techniques. The reconstructed CT-DE images show the implanted scaffold with the structural properties much clearer than those in the CT-PC and MR images. Furthermore, CT-DEI was able to visualize microstructures within the cartilage as well as different soft tissues surrounding the joint. These microstructural details were not recognizable using other imaging techniques. Taken together, the results of this study suggest that CT-DEI can be used for noninvasive visualization and characterization of scaffolds in cartilage, representing an advance in tissue engineering to track the success of tissue scaffolds for cartilage repair.

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“…Although developments are currently underway for nonsynchrotron‐based DEI systems, which use conventional X‐ray tubes, the images garnered with synchrotron X‐rays will always be held as the gold standard. Studies have been previously carried out on the efficacy of using DEI and phase‐contrast analyzer‐based imaging for the detection of cartilage lesions on disarticulated specimens, both with synchrotron (Li et al, ; Muehleman et al, ; Coan et al, 2010a,b) and nonsynchrotron X‐rays (Muehleman et al, b, ). DEI has even been studied for the evaluation of orthopedic implant integration into bone (Wagner et al, ), and a similarly based analyzer system using phase contrast has gone as far as the longitudinal in vivo imaging of guinea pigs for detection of osteoarthritic lesions (Coan et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…An area in which DEI has been particularly focused has been in the imaging of the cartilage and bone changes characteristic of osteoarthritis (OA) (Majumdar et al, 2004;Muehleman et al, 2006Muehleman et al, , 2009aMuehleman et al, ,b, 2010. The diagnosis of OA may be made as a patient begins to feel pain in the affected joint.…”

Section: Introductionmentioning

confidence: 99%

Assessment of diffraction‐enhanced synchrotron imaging for cartilage degeneration of the human knee joint

Wilson

Zelazny

et al. 2012

Clinical Anatomy

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Diffraction-enhanced imaging (DEI) is a radiographic technology that harnesses the X-ray refraction and scatter rejection properties that are not available with conventional radiography. Here, we test the efficacy of planar DEI to render images from which cartilage degeneration, characteristic of osteoarthritis, can be detected. DEI was carried out on human cadaveric intact knee joints at the X-15 beamline at the National Synchrotron Light Source. The gross specimens and the DEI images were graded separately for levels of cartilage degeneration on six individual surfaces: anterior and posterior femoral and tibial on both medial and lateral sides. There was a significant correlation between the actual levels of cartilage degeneration and what was observed in their respective DEI images (P < 0.05) for all six articular surfaces. Some articular surfaces (patellar surfaces, in particular) could not be visualized because of overlap with superimposed bone. Sensitivity for the graded articular surfaces was 0.73 and specificity was 0.92 (Grade 0 being no lesion and Grades 1-6 being increasing gradations of lesions). Chondrocalcinosis was also observed in DEI images to a far greater extent compared with the conventional radiographs. DEI renders images that are significantly correlated with their actual gross morphology. Detection of lesions was better for more severe grades of degeneration than for partial focal lesions. Although some articular surfaces could not be visualized because of superimposed bone, we feel that DEI has potential for the diagnosis of cartilage lesions and chondrocalcinosis.

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Preliminary study on diffraction enhanced radiographic imaging for a canine model of cartilage damage

Cited by 23 publications

References 27 publications

Preliminary Bone Imaging on 05B1-1 Beamline at the Canadian Light Source: Exploration of Diffraction Enhanced Imaging

Preliminary Bone Imaging on 05B1-1 Beamline at the Canadian Light Source: Exploration of Diffraction Enhanced Imaging

Computed Tomography Diffraction-Enhanced Imaging forIn SituVisualization of Tissue Scaffolds Implanted in Cartilage

Assessment of diffraction‐enhanced synchrotron imaging for cartilage degeneration of the human knee joint

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