Preliminary Study on Japanese Cedar Pollinosis in an Artificial Exposure Chamber (OHIO Chamber)

Hashiguchi, Kazuhiro; Tang, Herman; Fujita, Toshio; Tsubaki, Shigekazu; Fujita, Masami; Suematsu, Kiyochika; Gotoh, Minoru; Okubo, Kimihiro

doi:10.2332/allergolint.o-06-453

Cited by 19 publications

(23 citation statements)

References 8 publications

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“…These numbers are equivalent to the range of 3,000–12,000 determined by the KC-20 leaser counter used in the OHIO Chamber system [11]. Based on these findings, the pollen dispersal concentrations used in this study (maximum of 12,000 grains/m 3 ) are considered to be valid and not too high.…”

Section: Discussionmentioning

confidence: 99%

“…The aerosol was diluted with the outside air and dispersed in the Chamber. Air flow generator systems aimed at circulating the indoor air were set in the four corners of the Chamber, which allowed even distribution of the pollen at the target pollen dispersal concentration in the Chamber [11]. The target pollen dispersal concentrations were determined by means of the laser particle counter KC-20 (Rion, Tokyo, Japan), which allows real-time determination of the pollen concentration on the basis of the scattered light of laser illuminant.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, we established an environmental exposure unit (OHIO Chamber) designed to allow dispersal of JC pollen in Tokyo [11], which is the third unit established in this country after those in Wakayama [12] and Osaka [13]. …”

Section: Introductionmentioning

confidence: 99%

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Validation Study of the OHIO Chamber in Patients with Japanese Cedar Pollinosis

Hashiguchi

Tang²,

Fujita³

et al. 2009

Int Arch Allergy Immunol

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Background: An artificial exposure chamber (OHIO Chamber), which allows dispersal of a fixed concentration of Japanese cedar (JC) pollen under stable conditions, was constructed. This study was conducted to identify the exposure conditions assuring validity of the clinical tests conducted using this chamber. Methods: Twenty-four adult patients with JC pollinosis were exposed to different concentrations of JC pollen: 0 (only during the summer period), 4,000, 8,000 and 12,000 grains/m³, and the nasal and ocular symptoms were self-assessed during a 4-hour period of exposure. The amount of nasal discharge was measured and the sneezing frequency was recorded. This study was conducted twice during the summer and winter periods, i.e. non-pollen seasons. The reproducibility of the symptoms between the two seasons was assessed. Results: None of the subjects developed any symptom at the pollen concentration of 0 grains/m³. No significant differences in the time to the onset of symptoms were found between the summer and winter study, regardless of the pollen concentration. There were no significant differences between the summer and winter study in the total symptom score and total nasal symptom score at any pollen concentration, suggesting the very favorable reproducibility of symptoms. Conclusions: Efficient and reproducible results are obtained in patients exposed to JC pollen in the OHIO Chamber. The results suggest the conditions of JC pollen exposure have scientific validity and the OHIO Chamber has the potential to contribute significantly to basic and clinical studies of JC pollinosis.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Validation Study of the OHIO Chamber in Patients with Japanese Cedar Pollinosis

Hashiguchi

Tang²,

Fujita³

et al. 2009

Int Arch Allergy Immunol

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“…Hashiguchi et al (53) reported that artificial exposure to Japanese cedar pollen at 2500 pollen grains/m 3 , which is equivalent to the airborne pollen amount in the early pollinating season, caused penetration of 250 and 14 grains/h to the nose and eyes in humans. Airborne pollen amount during the middle and late pollinating season is ϳ5000 pollen grains/m 3 (53) and it could be estimated to cause penetration of 500 grains/h to the nose, which is equivalent to 5 g of grains/h (13,54).…”

Section: Discussionmentioning

confidence: 99%

Cupressaceae Pollen Grains Modulate Dendritic Cell Response and Exhibit IgE-Inducing Adjuvant Activity In Vivo

Kamijo¹,

Takai²,

Kuhara³

et al. 2009

The Journal of Immunology

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Pollen is considered a source of not only allergens but also immunomodulatory substances, which could play crucial roles in sensitization and/or the exacerbation of allergies. We investigated how allergenic pollens from different plant species (Japanese cedar and Japanese cypress, which belong to the Cupressaceae family, and birch, ragweed, and grass) modulate murine bone marrow-derived dendritic cell (DC) responses and examined the effect of Cupressaceae pollen in vivo using mice. DCs were stimulated with pollen extracts or grains in the presence or absence of LPS. Cell maturation and cytokine production in DCs were analyzed by flow cytometry, ELISA, and/or quantitative PCR. Pollen extracts suppressed LPS-induced IL-12 production and the effect was greatest for birch and grass. Without LPS, pollen grains induced DC maturation and cytokine production without IL-12 secretion and the response, for which TLR 4 was dispensable, was greatest for the Cupressaceae family. Intranasal administration of Cupressaceae pollen in mice induced an elevation of serum IgE levels and airway eosinophil infiltration. Coadministration of ovalbumin with Cupressaceae pollen grains induced ovalbumin-specific IgE responses associated with eosinophil infiltration. The results suggest that modulation of DC responses by pollen differs among the plant families via (1) the promotion of DC maturation and cytokine production by direct contact and/or (2) the inhibition of IL-12 production by soluble factors. The strong DC stimulatory activity in vitro and IgE-inducing activity in mice support the clinical relevance of Cupressaceae pollen to allergies in humans.

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“…We also started basic studies in July 2004 with the aim of constructing a system that could enable simultaneous exposure of multiple subjects to JC pollen antigen under uniform conditions at any time of year. Thus, our artificial exposure chamber (OHIO Chamber) was constructed at Samoncho Clinic in Yotsuya at the centre of Tokyo [11].…”

Section: Introductionmentioning

confidence: 99%

Pilot study of Japanese cedar pollen exposure using a novel artificial exposure chamber (OHIO Chamber)

Hashiguchi

Tang²,

Fujita³

et al. 2008

Clin Exp All Rev

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Summary For basic and clinical studies of Japanese cedar (JC) pollinosis and its treatment, experimental facilities for exposure to pollen under stable environmental conditions are becoming increasingly desirable. We developed an artificial exposure chamber (OHIO Chamber) that allows the dispersal of fixed concentrations of JC pollen in stable environments with a unique pollen supply system, air flow system for fixing the concentration of JC pollen, system for monitoring the number of pollen grains, and automated pure water washing and drying system. In the chamber, temperature and relative humidity (RH) could be successfully maintained at 22±1.1 °C and 45±5%, respectively. The spatial distribution of pollen concentrations in the chamber was within 10% of target, including when subjects were present. Only a few or no pollen grains were detected in the chamber after automatic washing and drying. We conducted a pilot tolerability and safety study in 15 JC pollinosis patients who were exposed to 15 000 pollen grains/m3 for 2 h. Symptoms manifested on average 33 min after start of exposure. The subjects experienced no serious side‐effects, and pollen exposure at 15 000 grains/m3 was confirmed safe. After exposure, the number of intranasal and intraocular pollen grains was 469 and 602, respectively. The lower number of pollen grains in the nose than in the eyes was considered due to sneezing and nasal discharge. Further studies are needed to clarify the number of pollen grains required for the occurrence of symptoms in the OHIO Chamber.

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Preliminary Study on Japanese Cedar Pollinosis in an Artificial Exposure Chamber (OHIO Chamber)

Abstract: This is the first clinical study using Japanese cedar pollen under well-controlled conditions in the OHIO chamber in which the induction of allergic symptoms was observed. The OHIO chamber will be useful for studying allergic rhinitis in Japan.

Cited by 19 publications

References 8 publications

Validation Study of the OHIO Chamber in Patients with Japanese Cedar Pollinosis

Validation Study of the OHIO Chamber in Patients with Japanese Cedar Pollinosis

Cupressaceae Pollen Grains Modulate Dendritic Cell Response and Exhibit IgE-Inducing Adjuvant Activity In Vivo

Pilot study of Japanese cedar pollen exposure using a novel artificial exposure chamber (OHIO Chamber)

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