Preliminary study on physiological changes of hemorrhagic shock in rats

Abstract

Hemorrhagic shock is a common cause of death in emergency rooms. The objective evaluation of hemorrhagic shock is very important for early diagnosis and treatment. The purpose of this study is to understand its mechanism by analyzing the changes of bio-signals in hemorrhagic shock using controlled hemorrhage of Sprague-Dawley rats. We constructed an integrated system to be able to control bleeding and to simultaneously measure bio-signals such as ECG, blood pressure, temperature, and respiration. In order to verify the system, we measured the bio-signals mentioned above while hemorrhagic shock was induced by withdrawing blood (2.5 ml / 100 g / 15 min) from a femoral vein for 10 rats.

Arterial input function sampling without surgery in rats for positron emission tomography molecular imaging

“…The volume of blood sampled also has an impact on the heart function: we observed a 12.5% decrease in the left ventricle ejection fraction between the single blood sampling (59.7±4.4%) and the dual blood sampling (unpaired t-test, P = 0.0013). Circulatory shock had an impact on the cardiac function and this is fairly well correlated to what we observe in our simultaneous experiment [7,9,30].…”

“…The importance of minimizing the amount of blood drawn from the animal was previously emphasized in order to avoid disturbing the animal's homeostasis as much as possible [9]. Collecting a large blood volume from the rat may result in decreased cardiac output, a drop in blood pressure, and decreased myocardial perfusion [7,9,30]. In this study we were able to document the effect of the withdrawn blood volume on the cardiac function and metabolism when blood was sampled simultaneously from the femoral and caudal arteries as compared with single artery sampling.…”

1H–NMR Metabolomic Biomarkers of Poor Outcome after Hemorrhagic Shock are Absent in Hibernators

“…Trauma is the third leading cause of death for persons under the age of 40 with approximately one third of these deaths resulting from HS [1], [2]. During HS the massive loss of blood reduces systemic blood pressure and produces I/R injury.…”