In this study, three different two-stage hybrid ecological wastewater treatment systems (HEWTS) with combinations of horizontal flow (HF) constructed wetlands (CWs), vertical flow (VF) CWs and stabilization ponds (SP) were evaluated for the removal of Organic-N, NH 4 + , NO 3 − , Total N, Total P, Total Coliforms (TCol) and Escherichia Coli, BOD, COD and TSS. The overall goal of the study was novel in comparison to most other studies in that we sought to evaluate and compare the efficiency of the three HEWTSs for water quality improvements, while minimizing nutrient removal from the wastewater in order to generate high quality reclaimed water for reuse for irrigation of crops. The most effective systems were those systems containing a vertical flow component, either HF-VF or VF-HF. In these two HEWTS, NH 4 + was reduced by 85.5% and 85.0% respectively, while NO 3 − was increased to 91.4 ± 17.6 mg/L and to 82.5 ± 17.2 mg/L, respectively, an artifact of nitrification. At the same time, E. coli was reduced by 99.93% and 99.99%, respectively. While the goal of most wastewater treatment is focused on reducing nutrients, the results here demonstrate that two-stage HEWTSs containing VF components can be used to produce a high quality effluent while retaining inorganic nutrients, thereby conserving this valuable resource for reuse as irrigation water for agriculture in subtropical developing countries where water and fertilizer resources are scarce or expensive.