Preliminary Study: Purple Sweet Potato Extract Seems to Be Superior to Increase the Migration of Impaired Endothelial Progenitor Cells Compared to l-Ascorbic Acid

Oktaviono, Yudi Her; Al-Farabi, Makhyan Jibril; Suastika, Luh Oliva Saraswati; Hartono, Febriyanti; Dirgantara, Y.; Sandra, Ferry

doi:10.3390/scipharm87030016

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…Raw images are available as Underlying data 22 . In this study, the use of FITC CD34+ only to confirmed the EPCs are sufficient, as the same EPCs culture method was used in authors previous research 20 , 23 – 25 . There was also uncertainty about the use of another immunophenotype of EPC as determined by flow cytometry (VEGFR2-PE, vWF-FITC, and CD31-PE), caused by heterogenous types of EPCs 26 , 27 …”

Section: Resultsmentioning

confidence: 99%

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Human umbilical cord blood-mesenchymal stem cell-derived secretome in combination with atorvastatin enhances endothelial progenitor cells proliferation and migration

et al. 2021

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Background: Human umbilical cord blood-mesenchymal stem cell (hUCB-MSC)-derived secretome is known to be able to promote neovascularization and angiogenesis, so it is also thought to have a capability to modulate endothelial progenitor cell (EPC) functions. Atorvastatin is the cornerstone of coronary artery disease (CAD) treatment which can enhance EPCs proliferation and migration. This study aims to analyze the effect of the hUCB-MSC-derived secretome and its combination with atorvastatin toward EPCs proliferation and migration. Methods: EPCs were isolated from a CAD patient’s peripheral blood. Cultured EPCs were divided into a control group and treatment group of 2.5 µM atorvastatin, hUCB-MSC-derived secretome (2%, 10%, and 20% concentration) and its combination. EPCs proliferation was evaluated using an MTT cell proliferation assay, and EPC migration was evaluated using a Transwell migration assay kit. Results: This research showed that hUCB-MSC-derived secretomes significantly increase EPC proliferation and migration in a dose-dependent manner. The high concentration of hUCB-MSC-derived secretome were shown to be superior to atorvastatin in inducing EPC proliferation and migration (p<0.001). A combination of the hUCB-MSC-derived secretome and atorvastatin shown to improve EPCs proliferation and migration compared to hUCB-MSC-derived secretome treatment or atorvastatin alone (p<0.001). Conclusions: This study concluded that the hUCB-MSC-derived secretome work synergistically with atorvastatin treatment in improving EPCs proliferation and migration.

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Section: Resultsmentioning

confidence: 99%

“…The MTT cell proliferation assay kit (Sigma-Aldrich, St Louis, MO, USA) was used to measure EPCs proliferation as described previously 20 . Treated EPCs were added with MTT reagent and incubated in a 37°C incubator with 5% CO 2 for 4 hours.…”

Section: Methodsmentioning

confidence: 99%

Human umbilical cord blood-mesenchymal stem cell-derived secretome in combination with atorvastatin enhances endothelial progenitor cells proliferation and migration

et al. 2021

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“…Previously, it has been shown that Vitamin C treatment alone can improve EPCs migration and proliferation. 25 Vitamin C improves EPCs proliferation and migration via suppression of ROS and pro-inflammatory cytokine (TNF-α in particular) which will reduce p-38 phosphorylation and stimulate…”

Section: Resultsmentioning

confidence: 99%

High Dose Allicin with Vitamin C Improves EPCs Migration from the Patient with Coronary Artery Disease

Oktaviono¹,

Amadis²,

Al-Farabi³

2020

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MATERIAL AND METHODS Allicin and Vitamin C preparation Garlic extract contains Allicin (ChemFaces ®) 98% was dissolved in dimethyl sulphoxide (DMSO). Vitamin C powder (Sigma Aldrich, USA) was dissolved in double-distilled water and diluted with culture medium to acquire the dose of 250 mcg/mL. Sample criteria The blood sample was acquired from eight CAD patients in Dr Soetomo General Hospital who fulfilled the inclusion criteria, which are: male, aged ABSTRACT Endothelial Progenitor Cells (EPCs) have an important role in endothelial dysfunction repairment through neovasculogenesis and cardiac myocytes regeneration. However, EPCs migration is greatly reduced in the patient with Coronary Artery Disease (CAD). Allicin and Vitamin C are hypothesized to improve EPCs migration due to its antioxidant properties. Objective: To investigate the effect of Allicin and its combination with Vitamin C in EPCs migration of CAD patients. Material and Method: Mononuclear cells were isolated from CAD patients and cultured on fibronectin-coated plates with colony-forming unit Hill medium. The cells were divided into untreated (control), Allicin treatment (dose 100 mcg/ml, 200 mcg/ml, 400 mcg/ ml), and each dose of Allicin combined with 250 mcg/mL of Vitamin C. EPCs migration was assessed with Transwell Migration Assay Kit and evaluated by using statistical tests. Results: This research shows that EPC migration was significantly higher in the treatment. Allicin at all dose (dose 100 mcg/ml, 200 mcg/ml, 400 mcg/ml) and its combination with 250 mcg/mL of vitamin C compared to untreated group (p<0.05). Allicin increase EPCs migration in a dosedependent manner. However, the only combination of 400 mcg/ml Allicin with 250 mcg/mL of vitamin C which has significantly higher EPCs migration compared to Allicin treatment alone. Conclusion: Allicin improves EPCs migration in a dose-dependent manner. Improvement of the migration only observed on the Allicin dose 400 mcg/ml with Vitamin C.

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Utilization of purple sweet potato as a partial substitute glutinous rice flour in the ombus-ombus cake from North Tapanuli traditional food

Sigalingging

Sinaga

Yusraini

2021

IOP Conf. Ser.: Earth Environ. Sci.

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Ombus-ombus is one of the traditional Batak Toba foods from North Tapanuli made from glutinous rice flour. The nutritional content of purple sweet potato is quite complete at affordable prices, which has the potential to be developed into processed food products based on local food and can reduce imports of Indonesian glutinous rice. The main objective of this research was to determine the best ombus-ombus cake formulation by using purple sweet potato as a partial substitute for glutinous rice flour. This research was conducted using a completely randomized design, consisted of two factors, factor I, the percentage substitute of purple sweet potato flour (P1 = 0%; P2 = 10%; P3 = 20%; P4 = 30%) and storage temperature condition (S1= at room temperature and S2= at freezing temperature) with three replications. The moisture content of ombus-ombus based on research was very significantly different (P <0.05) where the value was higher ranged 40-45%, and the organoleptic of colour, aroma, taste, texture were also very significantly different, almost the same value as previous research values and preferred by panellist than commercial products. The best formulation is 20% purple sweet potato flour and 80% glutinous rice flour with freezing storage.

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Preliminary Study: Purple Sweet Potato Extract Seems to Be Superior to Increase the Migration of Impaired Endothelial Progenitor Cells Compared to l-Ascorbic Acid

Cited by 4 publications

References 23 publications

Human umbilical cord blood-mesenchymal stem cell-derived secretome in combination with atorvastatin enhances endothelial progenitor cells proliferation and migration

Human umbilical cord blood-mesenchymal stem cell-derived secretome in combination with atorvastatin enhances endothelial progenitor cells proliferation and migration

High Dose Allicin with Vitamin C Improves EPCs Migration from the Patient with Coronary Artery Disease

Utilization of purple sweet potato as a partial substitute glutinous rice flour in the ombus-ombus cake from North Tapanuli traditional food

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