Prelude to oral microbes and chronic diseases: past, present and future

Atanasova, Kalina R.; Yılmaz, Özlem

doi:10.1016/j.micinf.2015.03.007

Cited by 65 publications

(81 citation statements)

References 71 publications

(159 reference statements)

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“…NDKs are small (~11–18 kDa), mainly cytoplasmic proteins, usually forming polymers (tetra- or hexamers) and having no known signal leader sequence, thus currently are unassigned to a specific secretion pathway234. The significance of NDK, including the human NDK species, encoded by the nm23 gene, has been suggested for the pathogenesis of several different forms of severe chronic diseases, including cancers567. In breast and other cancer types, the secretion of NDK from cancer cells has been shown to be critical for increased metastatic activity89.…”

mentioning

confidence: 99%

Nucleoside-Diphosphate-Kinase of P. gingivalis is Secreted from Epithelial Cells In the Absence of a Leader Sequence Through a Pannexin-1 Interactome

Atanasova

Lee

Roberts

et al. 2016

Sci Rep

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Nucleoside-diphosphate-kinases (NDKs) are leaderless, multifunctional enzymes. The mode(s) of NDK secretion is currently undefined, while extracellular translocation of bacterial NDKs is critical for avoidance of host pathogen clearance by opportunistic pathogens such as Porphyromonas gingivalis. P. gingivalis-NDK during infection inhibits extracellular-ATP (eATP)/P2X7-receptor mediated cell death in gingival epithelial cells (GECs) via eATP hydrolysis. Furthermore, depletion of pannexin-1-hemichannel (PNX1) coupled with P2X7-receptor blocks the infection-induced eATP release in GECs, and P. gingivalis-NDK impacts this pathway. Ultrastructural and confocal microscopy of P. gingivalis-co-cultured GECs or green-fluorescent-protein (GFP)-P. gingivalis-NDK transfected GECs revealed a perinuclear/cytoplasmic localization of NDK. eATP stimulation induced NDK recruitment to the cell periphery. Depletion of PNX1 by siRNA or inhibition by probenecid resulted in significant blocking of extracellular NDK activity and secretion using ATPase and ELISA assays. Co-immunoprecipitation-coupled Mass-spectrometry method revealed association of P. gingivalis-NDK to the myosin-9 motor molecule. Interestingly, inhibition of myosin-9, actin, and lipid-rafts, shown to be involved in PNX1-hemichannel function, resulted in marked intracellular accumulation of NDK and decreased NDK secretion from infected GECs. These results elucidate for the first time PNX1-hemichannels as potentially main extracellular translocation pathway for NDKs from an intracellular pathogen, suggesting that PNX1-hemichannels may represent a therapeutic target for chronic opportunistic infections.

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mentioning

confidence: 99%

Nucleoside-Diphosphate-Kinase of P. gingivalis is Secreted from Epithelial Cells In the Absence of a Leader Sequence Through a Pannexin-1 Interactome

Atanasova

Lee

Roberts

et al. 2016

Sci Rep

Self Cite

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“…This strongly suggests that in the healthy oral state, there is a large variety of yet-to-be-cultured bacteria unlike the bacterial consortium of individuals with changing (declining) oral health. In fact, studies have shown that some bacteria associated with health are poorly known, and include members of TM7 phylum, a bacterial group which does not have a single member cultured in the laboratory but which appears to be widely present in the oral cavity [12]. These data underscore the need to develop culture techniques that improve recovery of bacteria of a healthy human mouth while indicating that disturbed or oral disease mouths are replete of cultivable members.…”

Section: Discussionmentioning

confidence: 99%

“…How these community alterations play out in several diseases of the mouth, especially those involving mixed flora or a cascade of factors triggered by microbial or other signals, is poorly understood. The recent discovery of a novel bacterial co-prevalence pattern in dental carries -a supposedly previously well-characterized disease illustrates the dynamics of this phenomenon [12]. On the other hand, microbiome fingerprints in healthy persons are being examined for potential use in identity based on individuals' unique body microbes [13].…”

Section: Introductionmentioning

confidence: 99%

Metagenomic rDNA Profiles of the Oral Microbiome in Healthy Subjects Are Distinctive Compared to That in Smokers and Disease Subjects Despite High Variations between Subjects

Chakraborty¹,

Esiobu²

2017

AiM

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The vital role of oral microbiome in the well-being of humans is only beginning to be unraveled. Employing a rigorous analysis of PCR-restriction fragment length polymorphisms (PCR-RFLP) and DNA fingerprints from denatured gradient gel electrophoresis (PCR-DGGE) of the 16S rDNA gene in metagenomic samples, this study evaluated the stability of the oral microbiome and contrasted the PCR-DGGE profiles of subjects belonging to three groups -healthy, smokers and oral diseases; in search of distinctive patterns predictive of each group. The DNA band size, intensity and profile generated by three restriction enzymes from a 1500 bp amplicon showed a fairly stable microbial community structure (P < 0.05) in PCR-RFLP samples collected from each individual over a 3-month period. Microbial diversity indices and cluster analysis of amplicons from the V4 region of the 16S gene from the three study groups were consistent with a stable core of bacterial DNA fingerprint within and between subjects despite the enormous beta variations. Statistical analysis including multi-dimensional scaling of the DGGE fingerprints in smokers and oral disease subjects aligned both banding patterns (P-value 0.08, Student's t-test), suggesting some similarity between the microbial consortia of smokers and subjects with dental caries and gingivitis. There was a significant difference between bacterial genomic profiles in healthy mouth and smokers/ disease (P = 0.002; paired-sample Student's t-test). Operational taxonomic unit diversity and species richness determined by the GelCompare II software were higher in smokers ( H ′ = 0.99 ± 0.12; S = 2.87 ± 0.75) and oral disease mouths ( H ′ = 1.06 ± 0.085; S = 2.86 ± 0.31), than in healthy subjects ( H ′ = How to cite this paper: Chakraborty, S.

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“…A szájüregi flóra betegségekkel való összefüggése ismert, ám a mindennaposnak tűnő gondolat [7] jelentőségéhez képest mégis kevés figyelmet kap a modern medicinában. A normális flóra változatos előfordulási gyakorisággal, több száz különböző baktériumfajt tartalmaz [8][9][10], amelyek a szájüreg különböző pontjain és a fogak felszí-nén lévő biofilmben eltérő mértékben képesek megtelepedni.…”

Section: Megbeszélésunclassified

A szájüreg baktériumflórájának összefüggése az anastomosiselégtelenség következményeivel nyelőcső- és cardiatumoros betegeknél

Balázs

Winkler

Kristóf³

et al. 2017

Orvosi Hetilap

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Bevezetés: A tumor miatt végzett műtétek során készült nyelőcső-anastomosisok elégtelensége kapcsán az insufficientián át kilépő tartalom bakteriális összetételének a szövődmény kimenetelében jelentős befolyásoló szerepét tételez-hetjük fel. Célkitűzés: A vizsgálat célja az anastomosiselégtelenség következményeinek összevetése a betegek száj üregi bakteriális flórájával. Módszer: A prospektív vizsgálat során 131, műtétre került betegnél közvetlenül a műtét előtti órákban mintavételt végeztünk. A betegek szájüregi flórájának összetételét elemeztük. Az anastomosiselégtelenség következményei és a baktériumflóra összetétele közötti korrelációt vizsgáltuk. Eredmények: Patogén baktérium jelenléte a szájüregi flórában 50 esetben (38,2%) volt kimutatható. A szövődmény súlyossága és a patogén baktériumok előfordulási gyakorisága között a korrelációanalízis közepes erősségű kapcsolatot mutatott ki (r s = 0,553), az eredmény szignifikáns (p≤0,05). Következtetések: A patogén ágenseket tartalmazó flóra a varratelégtelenség eseteiben nagyobb kockázatot és súlyosabb kimenetelt eredményezhet, a meghatározó tényezők egyikeként értékelhető. A száj-üregi bakteriális flóra a műtéti előkészítés során a korábbiaknál nagyobb figyelmet és a műtéti előkészítés gyakorlatának megváltoztatását igényli. Orv. Hetil., 2017, 158(1), 25-30.Kulcsszavak: szájüregi baktériumflóra, nyelőcsőtumor, anastomosiselégtelenség, műtéti előkészítés Relationship of consequences of anastomotic insufficiency and bacterial flora of oral cavity in patients with esophageal and cardia cancerIntroduction: In the course of anastomotic insufficiency following resection of esophageal cancers the bacterial compound of the esophageal substance has a remarkable, presumable role in the outcome of complications. Aim: The purpose of this study is to compare the consequences of the anastomotic leak with the bacterial flora of patients' oral cavity. Method: In this prospective study a total of 131 patients were investigated directly before the surgical intervention taking a bacterial sample. Bacterial flora of patients' oral cavity was analysed; and the correlation between the consequences of the anastomotic leak and the content of the bacterial flora was examined. Results: Pathogenic bacteria in the oral microflora in 50 cases (38.2%) was found. Statistically significant, moderate correlation was found between the severity of the complication and the incidence of pathogenic bacteria (r s = 0.553; p≤0.05). Conclusions: Pathogenic agent in the microbial flora might induce higher risk and more severe outcome in case of anastomotic leakage and it might be evaluated as a determinative factor. Consideration of the bacterial flora of the oral cavity requires more attention in the preoperative preparation than before and it demands the change of the current practice.

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Prelude to oral microbes and chronic diseases: past, present and future

Cited by 65 publications

References 71 publications

Nucleoside-Diphosphate-Kinase of P. gingivalis is Secreted from Epithelial Cells In the Absence of a Leader Sequence Through a Pannexin-1 Interactome

Nucleoside-Diphosphate-Kinase of P. gingivalis is Secreted from Epithelial Cells In the Absence of a Leader Sequence Through a Pannexin-1 Interactome

Metagenomic rDNA Profiles of the Oral Microbiome in Healthy Subjects Are Distinctive Compared to That in Smokers and Disease Subjects Despite High Variations between Subjects

A szájüreg baktériumflórájának összefüggése az anastomosiselégtelenség következményeivel nyelőcső- és cardiatumoros betegeknél

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