PREM-1, a putative maize retroelement has LTR (long terminal repeat) sequences that are preferentially transcribed in pollen

Turcich, Michael P.; Mascarenhas, Joseph P.

doi:10.1007/bf00241882

Cited by 18 publications

(15 citation statements)

References 23 publications

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“…However, potential genes that have similarities to components of retroelements are probably reflecting the fact that intergenic space is frequently occupied by retroelements. This is clearly the case between azs22;5 and azs22;6, where a sequence related to the element Prem-1 is found (Turcich and Mascarenhas, 1994). This intergenic space also contains the largest non-zein, continuous openreading-frame with 464 amino acids.…”

Section: Discussionmentioning

confidence: 85%

Amplicons of maize zein genes are conserved within genic but expanded and constricted in intergenic regions

Llaca

Messing

1998

The Plant Journal

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Section: Discussionmentioning

confidence: 85%

Amplicons of maize zein genes are conserved within genic but expanded and constricted in intergenic regions

Llaca

Messing

1998

The Plant Journal

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“…Two retroelements, Prem1 and Prem2, whose expression is limited to the male microspore (Turcich & Mascarenhas 1994, Turcich et al 1996 were found in many of the clones sequenced in this study. In the A genome, sequences from the Prem2 element have been co-opted for tissue-specific regulation of several polygalacturonase genes which are expressed in pollen (Turcich et al 1996).…”

Section: Transcription Of B Chromosome-specific Sequencesmentioning

confidence: 90%

Localization and transcription of a retrotransposon-derived element on the maize B chromosome

et al. 2007

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Dispensable chromosomes in addition to the normal complement in diverse taxa are called B chromosomes. The maize B chromosome is discernible in mitotic chromosome spreads as a small compact chromosome composed mainly of heterochromatin. Although much of this chromosome consists of repetitive elements common to the A chromosomes, several sequences specific to the B chromosome have been identified. In the work described here we used the sequence from a B-specific RAPD (random amplification of polymorphic DNA) marker, pBGBM18.2, to isolate another DNA element, StarkB, present in many copies on the B chromosome. StarkB was mapped to the third and fourth blocks of distal heterochromatin using translocation breakpoints and fluorescent in-situ hybridization (FISH). Sequence analysis revealed that StarkB is composed of fragments from the A genome as well as B-specific sequences. The StarkB element is much larger than the other B-specific elements and is not present in large tandem arrays. Different copies of StarkB varied by small insertions, deletions, and duplications as well as single-nucleotide polymorphisms. Reverse transcriptase PCR showed that portions of the StarkB element are expressed. Using the LTR divergence of retroelements interrupting the B-specific sequences, the minimum age of the StarkB repeat array and, by inference, of the B chromosome, was estimated to be 2 million years.

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“…PREM-1 (pollen retroelement of maize) LTRs are present as dispersed repeats at a copy number of 10 000 to 40 000 in maize. Turcich & Mascarenhas (1994) highly similar sequences are specific to the genus Zea (maize and teosinte). The PREM-1 LTRs are primarily transcribed during pollen grain development, and it is interesting to note that gene expression determined by the B is also thought to occur during male gametogenesis, leading to the directed non-disjunction process.…”

Section: Molecular Analysis Of Maize B-chromosomementioning

confidence: 98%

Molecular analysis of the structure of the maize B-chromosome

et al. 1996

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The overall composition of the maize B is similar to that of the standard chromosome complement (A-chromosomes). This has been demonstrated by the use of several methods including: (a) genomic in situ hybridization (GISH), (b) analysis of highly repetitive sequences by the comparison of restriction digests of 0B and +B DNA and (c) the characterization of middle-repetitive cloned sequences. By the use of the technique of random amplification of polymorphic DNA-polymerase chain reaction, we have identified a B-specific repetitive sequence family. Sequence analysis of the B-specific clone reveals a relationship to the PREM-1 family of maize retroelements, which are preferentially transcribed in pollen. These results suggest an internal origin of the B-chromosome from within the maize genome, but demonstrate also that specific sequences can evolve by rapid processes of genomic turnover. Models for the origin of the maize B are discussed in the context of the present data.

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PREM-1, a putative maize retroelement has LTR (long terminal repeat) sequences that are preferentially transcribed in pollen

Cited by 18 publications

References 23 publications

Amplicons of maize zein genes are conserved within genic but expanded and constricted in intergenic regions

Amplicons of maize zein genes are conserved within genic but expanded and constricted in intergenic regions

Localization and transcription of a retrotransposon-derived element on the maize B chromosome

Molecular analysis of the structure of the maize B-chromosome

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