Prenatal acoustic stimulation influences neuronal size and the expression of calcium-binding proteins (calbindin D-28K and parvalbumin) in chick hippocampus

Chaudhury, Sraboni; Nag, Tapas Chandra; Wadhwa, Shashi

doi:10.1016/j.jchemneu.2006.07.002

Cited by 32 publications

(21 citation statements)

References 54 publications

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“…Early maturation of the auditory system [13]–[15] and the development of species typical perceptual preference is observed in birds following exposure to species own typical call [16], [17]. Our previous studies showed improved morphological and biochemical changes in chick hippocampus and facilitation of spatial orientation and learning at 12 hour post hatch consequent to prenatal sound stimulation by species-specific calls and sitar music at 65 dB [18]–[21]. The facilitation of learning was observed even at 24 hour post hatch with no further improvement in successive trials following the same sound enrichment protocol suggesting an early maturation of synaptic connectivity following prenatal sound stimulation.…”

Section: Introductionmentioning

confidence: 99%

Prenatal Loud Music and Noise: Differential Impact on Physiological Arousal, Hippocampal Synaptogenesis and Spatial Behavior in One Day-Old Chicks

et al. 2013

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Prenatal auditory stimulation in chicks with species-specific sound and music at 65 dB facilitates spatial orientation and learning and is associated with significant morphological and biochemical changes in the hippocampus and brainstem auditory nuclei. Increased noradrenaline level due to physiological arousal is suggested as a possible mediator for the observed beneficial effects following patterned and rhythmic sound exposure. However, studies regarding the effects of prenatal high decibel sound (110 dB; music and noise) exposure on the plasma noradrenaline level, synaptic protein expression in the hippocampus and spatial behavior of neonatal chicks remained unexplored. Here, we report that high decibel music stimulation moderately increases plasma noradrenaline level and positively modulates spatial orientation, learning and memory of one day-old chicks. In contrast, noise at the same sound pressure level results in excessive increase of plasma noradrenaline level and impairs the spatial behavior. Further, to assess the changes at the molecular level, we have quantified the expression of functional synapse markers: synaptophysin and PSD-95 in the hippocampus. Compared to the controls, both proteins show significantly increased expressions in the music stimulated group but decrease in expressions in the noise group. We propose that the differential increase of plasma noradrenaline level and altered expression of synaptic proteins in the hippocampus are responsible for the observed behavioral consequences following prenatal 110 dB music and noise stimulation.

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Section: Introductionmentioning

confidence: 99%

Prenatal Loud Music and Noise: Differential Impact on Physiological Arousal, Hippocampal Synaptogenesis and Spatial Behavior in One Day-Old Chicks

et al. 2013

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“…In rats, prenatal exposure to music results in an increased neurogenesis in the hippocampus and is associated with enhanced spatial memory (Kim et al, 2006), suggesting an important role for prenatal auditory stimulation in the development of neonates. Earlier studies from our laboratory showed beneficial morphological and biochemical changes in two brainstem auditory nuclei, viz., nucleus magnocellularis (NM) and nucleus laminaris (NL), the auditory imprinting area, mediorostral neostriatum/hyperstriatum ventrale (MNH) (according to new nomenclature, nidopallium and mesopallium, Reiner, 2005), and the hippocampus (Wadhwa et al, 1999; Alladi et al, 2002, 2005a,b; Panicker et al, 2002; Chaudhury and Wadhwa, 2009; Chaudhury et al, 2006, 2008, 2009, 2010) following prenatal auditory stimulation in chicks with species specific call and sitar music at ambient sound pressure level (65 decibel [dB]). On detailed comparisons, it was found that following species specific sound stimulation, which was discontinuous in nature, the neuronal nuclear size increased in the auditory nuclei and MNH while the hippocampal neurons did not show significant changes.…”

Section: Introductionmentioning

confidence: 99%

Effect of prenatal loud music and noise on total number of neurons and glia, neuronal nuclear area and volume of chick brainstem auditory nuclei, field L and hippocampus: A stereological investigation

Sanyal

Palanisamy

Nag

et al. 2013

Intl J of Devlp Neuroscience

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The present study explores whether prenatal patterned and unpatterned sound of high sound pressure level (110 dB) has any differential effect on the morphology of brainstem auditory nuclei, field L (auditory cortex analog) and hippocampus in chicks (Gallus domesticus). The total number of neurons and glia, mean neuronal nuclear area and total volume of the brainstem auditory nuclei, field L and hippocampus of post-hatch day 1 chicks were determined in serial, cresyl violet-stained sections, using stereology software. All regions studied showed a significantly increased total volume with increase in total neuron number and mean neuronal nuclear area in the patterned music stimulated group as compared to control. Contrastingly the unpatterned noise stimulated group showed an attenuated volume with reduction in the total neuron number. The mean neuronal nuclear area was significantly reduced in the auditory nuclei and hippocampus but increased in the field L. Glial cell number was significantly increased in both experimental groups, being highest in the noise group. The brainstem auditory nuclei and field L showed an increase in glia to neuron ratio in the experimental groups as compared to control. In the hippocampus the ratio remained unaltered between control and music groups, but was higher in the noise group. It is thus evident that though the sound pressure level in both experimental groups was the same there were differential changes in the morphological parameters of the brain regions studied, indicating that the characteristics of the sound had a role in mediating these effects.

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“…The increased levels of CREB and BDNF mRNA as well as p‐CREB and BDNF protein in both the auditory stimulated groups may enhance the survival, proliferation, differentiation and maturation of neurons. In fact, our earlier study on the chick hippocampus (Chaudhury et al, 2006) showed an increase in the number of neurons at PH1 in the auditory stimulated groups.…”

Section: Discussionmentioning

confidence: 82%

“…BDNF mRNA expression is also upregulated by the neuronal activity that is accompanied by Ca 2+ influx into the post‐synaptic spines of neurons (Ghosh et al, 1994). In our earlier studies, we have observed an increase in the expression and levels of two calcium‐binding proteins (calbindin and parvalbumin) following prenatal sound stimulation, which is an indirect indication of Ca 2+ influx (Chaudhury et al, 2006, 2008, 2009). Therefore, it appears that the calcium mediated pathway is enhanced via CREB phosphorylation and results in increased levels of BDNF.…”

Section: Discussionmentioning

confidence: 90%

Prenatal auditory stimulation alters the levels of CREB mRNA, p‐CREB and BDNF expression in chick hippocampus

Chaudhury

Wadhwa

2009

Intl J of Devlp Neuroscience

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Prenatal auditory stimulation influences the development of the chick auditory pathway and the hippocampus showing an increase in various morphological parameters as well as expression of calcium-binding proteins. Calcium regulates the activity of cyclic adenosine monophosphate-response element binding (CREB) protein. CREB is known to play a role in development, undergo phosphorylation with neural activity as well as regulate transcription of BDNF. BDNF is important for the survival of neurons and regulates synaptic strength. Hence in the present study, we have evaluated the levels of CREB mRNA and protein along with p-CREB protein as well as BDNF mRNA and protein levels in the chick hippocampus at embryonic days (E) 12, E16, E20 and post-hatch day (PH) 1 following activation by prenatal auditory stimulation. Fertilized eggs were exposed to species-specific sound or sitar music (frequency range: 100-6300Hz) at 65dB levels for 15min/h over 24h from E10 till hatching. The control chick hippocampus showed higher CREB mRNA and p-CREB protein in the early embryonic stages, which later decline whereas BDNF mRNA and BDNF protein levels increase until PH1. The CREB mRNA and p-CREB protein were significantly increased at E12, E16 and PH1 in the auditory stimulated groups as compared to control group. A significant increase in the level of BDNF mRNA was observed from E12 and the protein expression from E16 onwards in both auditory stimulated groups. Therefore, enhanced phosphorylation of CREB during development following prenatal sound stimulation may be responsible for cell survival. Increased levels of p-CREB again at PH1 may trigger synthesis of proteins necessary for synaptic plasticity. Further, the increased levels of BDNF may also help in regulating synaptic plasticity.

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Prenatal acoustic stimulation influences neuronal size and the expression of calcium-binding proteins (calbindin D-28K and parvalbumin) in chick hippocampus

Cited by 32 publications

References 54 publications

Prenatal Loud Music and Noise: Differential Impact on Physiological Arousal, Hippocampal Synaptogenesis and Spatial Behavior in One Day-Old Chicks

Prenatal Loud Music and Noise: Differential Impact on Physiological Arousal, Hippocampal Synaptogenesis and Spatial Behavior in One Day-Old Chicks

Effect of prenatal loud music and noise on total number of neurons and glia, neuronal nuclear area and volume of chick brainstem auditory nuclei, field L and hippocampus: A stereological investigation

Prenatal auditory stimulation alters the levels of CREB mRNA, p‐CREB and BDNF expression in chick hippocampus

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