Prenatal alcohol exposure in the second trimester-equivalent increases the seizure susceptibility in developing rats

Cho, Sue J.; Newton, Jamila; Li, Tengfei; Khandai, Padmini; Luta, George; Lovinger, David M.; N’Gouemo, Prosper

doi:10.1016/j.alcohol.2020.01.005

Cited by 3 publications

(7 citation statements)

References 33 publications

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“…Afterward, the cerebral damages were principally triggered within the right hemisphere. Therefore, we tend to principally use the anemia ipsilateral brain (right cerebral hemisphere) within the later detection 31 …”

Section: Discussionmentioning

confidence: 99%

“…In accordance with the previous literature, the P7 neonatal rats suffered from unilateral carotid artery occlusion to establish the NHIE model in this study. P7 rats were selected because the peak brain growth occurs for rats at this time point, which would appear at full‐term in humans 31,32 . In addition, this is the time when cortical myelination for rodents just starts, equivalent to 34 weeks of human pregnancy 33 .…”

Section: Discussionmentioning

confidence: 99%

“…P7 rats were selected because the peak brain growth occurs for rats at this time point, which would appear at full‐term in humans. 31 , 32 In addition, this is the time when cortical myelination for rodents just starts, equivalent to 34 weeks of human pregnancy. 33 In the present study, P7 neonatal rats were chosen for ligation of the correct arteria carotid artery following 2 h hypoxia.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, we tend to principally use the anemia ipsilateral brain (right cerebral hemisphere) within the later detection. 31 4.2 | Downregulation of AQP4 improved axon regrowth and long-term neural behavior after HIE…”

Section: P7 Rats Were Selected For Nhie Model Establishmentmentioning

confidence: 99%

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AQP4 knockout promotes neurite outgrowth via upregulating GAP43 expression in infant rats with hypoxic‐ischemic brain injury

Dan

Tan

et al. 2022

Ibrain

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Neonatal hypoxic‐ischemic encephalopathy (NHIE) induces severe cerebral damage and neurological dysfunction, with seldom effective therapy. Aquaporin‐4 (AQP4) is involved in aggravating brain damage induced by NHIE. This study aimed to investigate the role of AQP4 underlying the pathogenesis of NHIE. Neonatal Sprague–Dawley rats were used to establish neonatal hypoxic‐ischemic (HI) models, and the expression of AQP4 in the cortex, hippocampus, and lung tissues was detected by real‐time quantitative polymerase chain reaction as well as Western blot. Primary cortical neurons were cultured for the oxygen‐glucose deprivation (OGD) model, and siRNA was used to silence the expression of AQP4. Immunostaining of Tuj1 was performed to observe the axonal growth. CRISPER/Cas9 technology was used to knock out AQP4. The results demonstrated that AQP4 was upregulated in the cortex, hippocampus, and lung tissues in neonatal rats with HI and OGD neurons. Besides, silencing AQP4 promoted axonal growth of OGD neurons, and AQP4 knockout notably improved long‐term neurobehavioral impairment. Furthermore, GAP43 was found closely correlated with AQP4 via GeneMANIA prediction. Significant downregulation of GAP43 was induced in OGD neurons, while AQP4 knockout markedly upregulated its expression in rats. This indicated that the depletion of AQP4 may enhance axonal regeneration and promote the long‐term neurobehavioral recovery associated with the upregulation of GAP43 expression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: P7 Rats Were Selected For Nhie Model Establishmentmentioning

confidence: 99%

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AQP4 knockout promotes neurite outgrowth via upregulating GAP43 expression in infant rats with hypoxic‐ischemic brain injury

Dan

Tan

et al. 2022

Ibrain

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“…A exposição pré-natal ao álcool causa diversos efeitos deletérios, como a indução de doenças coronárias (Chen et al, 2021), maior disposição a convulsões (Cho et al, 2020), além de ser um fator de risco a dependência alcoólica e problemas relacionados ao uso de álcool durante a juventude (Baer et al, 2015). A SAF, doença mais grave no espectro do TEAF, possui uma característica que a diferencia das demais: ela causa dismorfologias faciais e cerebrais (Lipinski et al, 2012).…”

Section: Introductionunclassified

Medicamentos Com Excipiente Alcoólico Administrados a Neonatos No Sistema De Saúde Brasileiro

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The Extracellular Vesicle Citrullinome and Signature in a Piglet Model of Neonatal Seizures

Mitra

Harvey-Jones

Kraev

et al. 2023

IJMS

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Neonatal seizures are commonly associated with acute perinatal brain injury, while understanding regarding the downstream molecular pathways related to seizures remains unclear. Furthermore, effective treatment and reliable biomarkers are still lacking. Post-translational modifications can contribute to changes in protein function, and post-translational citrullination, which is caused by modification of arginine to citrulline via the calcium-mediated activation of the peptidylarginine deiminase (PAD) enzyme family, is being increasingly linked to neurological injury. Extracellular vesicles (EVs) are lipid-bilayer structures released from cells; they can be isolated from most body fluids and act as potential liquid biomarkers for disease conditions and response to treatment. As EVs carry a range of genetic and protein cargo that can be characteristic of pathological processes, the current study assessed modified citrullinated protein cargo in EVs isolated from plasma and CSF in a piglet neonatal seizure model, also following phenobarbitone treatment. Our findings provide novel insights into roles for PAD-mediated changes on EV signatures in neonatal seizures and highlight the potential of plasma- and CSF-EVs to monitor responses to treatment.

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Prenatal alcohol exposure in the second trimester-equivalent increases the seizure susceptibility in developing rats

Cited by 3 publications

References 33 publications

AQP4 knockout promotes neurite outgrowth via upregulating GAP43 expression in infant rats with hypoxic‐ischemic brain injury

AQP4 knockout promotes neurite outgrowth via upregulating GAP43 expression in infant rats with hypoxic‐ischemic brain injury

Medicamentos Com Excipiente Alcoólico Administrados a Neonatos No Sistema De Saúde Brasileiro

The Extracellular Vesicle Citrullinome and Signature in a Piglet Model of Neonatal Seizures

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