Prenatal Diagnosis of Congenital Toxoplasmosis

Desmonts, G; Forestier, F.; Thulliez, P.; Daffos, F.; Capella-Pavlovsky, M; Chartier, M.

doi:10.1016/s0140-6736(85)92096-3

Cited by 211 publications

(87 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In a study of 112 placentas from immunocompetent women who were seropositive for T. gondii before pregnancy, no organisms could be found in the placenta [33]. In another study of 177 pregnancies that were terminated, women were separated into two categories; 62 women who acquired infection before or soon after conception and 115 women who acquired infection during pregnancy [213]. Of the women who acquired infection during pregnancy 10 out of 115 had organisms in the placenta, while no organisms were found in the placenta of the 62 women who acquired infection before or at conception.…”

Section: Congenital Toxoplasmosismentioning

confidence: 99%

“…The later maternal infection is acquired during pregnancy, the more frequently parasites are transmitted to the foetus. In the last few weeks of gestation the transmission rate rises to greater than 90%, while in the first 6 weeks after conception the rate is under 2% [213][214][215]. An average transmission rate for each trimester in which seroconversion occurs is 14% for the first trimester, 30% for the second trimester, and 59% for the third trimester.…”

Section: Congenital Toxoplasmosismentioning

confidence: 99%

See 1 more Smart Citation

Toxoplasma gondii: from animals to humans

Tenter

Heckeroth

Weiss

2000

International Journal for Parasitology

3,011

2,476

View full text Add to dashboard Cite

Toxoplasmosis is one of the more common parasitic zoonoses world-wide. Its causative agent, Toxoplasma gondii, is a facultatively heteroxenous, polyxenous protozoon that has developed several potential routes of transmission within and between different host species. If first contracted during pregnancy, T. gondii may be transmitted vertically by tachyzoites that are passed to the foetus via the placenta. Horizontal transmission of T. gondii may involve three lifecycle stages, i.e. ingesting infectious oocysts from the environment or ingesting tissue cysts or tachyzoites which are contained in meat or primary offal (viscera) of many different animals. Transmission may also occur via tachyzoites contained in blood products, tissue transplants, or unpasteurised milk. However, it is not known which of these routes is more important epidemiologically. In the past, the consumption of raw or undercooked meat, in particular of pigs and sheep, has been regarded as a major route of transmission to humans. However, recent studies showed that the prevalence of T. gondii in meat-producing animals decreased considerably over the past 20 years in areas with intensive farm management. For example, in several countries of the European Union prevalences of T. gondii in fattening pigs are now <1%. Considering these data it is unlikely that pork is still a major source of infection for humans in these countries. However, it is likely that the major routes of transmission are different in human populations with differences in culture and eating habits. In the Americas, recent outbreaks of acute toxoplasmosis in humans have been associated with oocyst contamination of the environment. Therefore, future epidemiological studies on T. gondii infections should consider the role of oocysts as potential sources of infection for humans, and methods to monitor these are currently being developed. This review presents recent epidemiological data on T. gondii, hypotheses on the major routes of transmission to humans in different populations, and preventive measures that may reduce the risk of contracting a primary infection during pregnancy.

show abstract

Section: Congenital Toxoplasmosismentioning

confidence: 99%

Section: Congenital Toxoplasmosismentioning

confidence: 99%

Toxoplasma gondii: from animals to humans

Tenter

Heckeroth

Weiss

2000

International Journal for Parasitology

3,011

2,476

View full text Add to dashboard Cite

show abstract

“…inoculation of mice [1][2][3] and on serological tests, which detect anti-toxoplasma IgG and IgM Correspondence: Dr Anne Decoster, Laboratoire St Antoine, 329 Boulevard Victor Hugo, BP 255, 59019 Lille Cedex, France.…”

Section: Introductionmentioning

confidence: 99%

Anti-P30 IgA antibodies as prenatal markers of congenital toxoplasma infection

Decoster

Darcy

Caron

et al. 1992

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYThis study extends a previous study and confirms that the detection of anti-P30 IgA antibodies is very helpful in the diagnosis of acute acquired or congenital toxoplasmosis. Moreover, we demonstrate that an anti-P30 IgA response can be mounted in the fetuses infected by Toxoplasma gondii during their intra-uterine life as early as week 23 of gestation. A double-sandwich ELISA described in our previous work was used to detect anti-P30 IgA antibodies in 1378 human serum samples collected from 551 patients, including 162 fetuses whose mothers had been infected by T. gondii during pregnancy, 46 congenitally infected and 90 uninfected newborns and 253 women suspected of having been infected during pregnancy, including the mothers of fetuses and newborns previously described. Anti-P30 IgA antibodies were detected in all cases of acute toxoplasmosis but in no case of chronic toxoplasmosis: in the majority of cases, the IgA antibody titre fell below cut-off in 3-9 months. Among the 46 congenitally infected newborns, anti-P30 IgA antibodies were detected in sera of 41 infected newborns (38 at birth, two in the first months of life, one in the seventh month of life), while anti-P30 IgM antibodies were detected in only 30 cases at birth and in one case during the first month of life. Among 162 fetuses, anti-P30 IgA response was observed in five infected fetuses, but was not detected in either 152 uninfected fetuses or in five fetuses considered as infected. The absence or presence of anti-P30 IgA antibodies in the fetus is discussed in relation to the date of maternal infection and collection ofthe fetal blood. It clearly appears from our study that the combined testing of both IgM and IgA in the fetus and the newborn is essential for a more efficient diagnosis of infection.

show abstract

“…Early diagnosis is important in patients who present clinical symptoms of toxoplasmosis and in pregnant women to prevent severe congenital infection in the developing fetus (24,25). Toxoplasmosis diagnosis depends on direct and indirect methods.…”

Section: Discussionmentioning

confidence: 99%

Novel IMB-ELISA Assay for Rapid Diagnosis of Human Toxoplasmosis Using SAG1 Antigen

et al. 2015

View full text Add to dashboard Cite

SUMMARY: Nanotechnology is important for medical diagnosis. Various nanoparticles have presented tremendous potential for diagnosing disease markers, pre-cancerous cells, fragment of viruses, specific proteins, antibodies, and other disease indicators. In general, nanoparticles are smaller than 1,000 nm and produced from different materials in different shapes such as spheres, rods, wires, and tubes. Our study aimed to develop a novel antigen-capture immunoassay based on IgG polyclonal antibody-coated magnetic microbead nanoparticles for the rapid detection of circulating surface antigen 1 of Toxoplasma gondii in human serum samples. Sandwich ELISA elicited a sensitivity of 92z, a specificity of 92.7z, a positive predictive value (PPV) of 92z, and a negative predictive value (NPV) of 92.7z. Immunomagnetic bead-ELISA showed sensitivity (98z), specificity (96.4z), PPV (96z), and NPV (98.1z) higher than that of sandwich ELISA. It is obvious that the use of magnetic microbead nanoparticles offers the potential advantage of improving the diagnostic testing of toxoplasmosis.

show abstract

Prenatal Diagnosis of Congenital Toxoplasmosis

Cited by 211 publications

References 9 publications

Toxoplasma gondii: from animals to humans

Toxoplasma gondii: from animals to humans

Anti-P30 IgA antibodies as prenatal markers of congenital toxoplasma infection

Novel IMB-ELISA Assay for Rapid Diagnosis of Human Toxoplasmosis Using SAG1 Antigen

Contact Info

Product

Resources

About