Prenatal sex detection with endocervical smears: Successful results utilizing Y-body fluorescence

Rhine, Samuel A.; Cain, Jeffrey L.; Cleary, Robert E.; Palmer, Catherine G.; Thompson, Joseph F.

doi:10.1016/s0002-9378(16)33486-x

Cited by 43 publications

(19 citation statements)

References 8 publications

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“…Several more studies were published up to 1980 with conflicting results (Bobrow and Lewis, 1971;Warren et al, 1971;Tsuji and Sasaki, 1973;Goldstein et al, 1973;Manuel et al, 1974;Rhine et al, 1975Rhine et al, , 1977Rhine and Milunsky, 1979;Amankwah and Bond, 1977;Varner et al, 1977;Goldberg et al, 1980), reviewed by Adinolfi (1995). More recently, the emergence of new technology, e.g.…”

Section: Discussionmentioning

confidence: 99%

Detection of Cells of Fetal Origin From Transcervical Irrigations

1997

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Section: Discussionmentioning

confidence: 99%

Detection of Cells of Fetal Origin From Transcervical Irrigations

1997

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“…The slide was prepared as describe by Imudia et al (18) from a specimen obtained during the first trimester of pregnancy. Antibody labeling was detected using monoclonal antibody G233 against HLA-G and a secondary antibody conjugated to peroxidase to produce a dark brown precipitate around the cytotrophoblast cell.…”

Section: Figures and Tablesmentioning

confidence: 99%

Transcervical retrieval of fetal cells in the practice of modern medicine: a review of the current literature and future direction

Imudia

Kumar

Diamond

et al. 2010

Fertility and Sterility

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Objective-To review published methods for transcervical collection of fetal cells and to assess the potential of this approach for application in prenatal diagnosis.Design-Retrospective analysis of efforts at prenatal diagnosis using trophoblast cells shed into the lower uterine pole that accumulate within the cervical mucus at the level of the internal os.Results-Minimally invasive techniques that include cervical mucus aspiration, cervical swabbing, and cervical or intrauterine lavage can be used to retrieve trophoblast cells during the first trimester for diagnostic purposes, including prenatal genetic analysis. Fetal cells have been identified in these specimens with success rates that vary from 40 to 90%. The disparity in reported success rates can be a function of gestational age, collection method, operator variability, detection sensitivity, or pregnancy status. Molecular approaches have been devised to determine fetal sex and identify aneuploidies. Antibody markers have proven useful to select trophoblast cells for genetic analysis and to demonstrate that the abundance of recoverable fetal cells diminishes in abnormal gestations, such as ectopic pregnancy and blighted ovum.Conclusions-Transcervical collection of fetal cells offers several avenues for prenatal diagnosis that with further refinement could one day provide valuable information for the management of ongoing pregnancies.

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“…Trophoblastic cells have repeatedly been shown to be shed into the uterine cavity and then into the endocervical canal between five and 15 weeks of gestation (Shettles, 1971;Rhine et al, 1975Rhine et al, , 1977Rhine et al, , 1979Griffith-Jones et al, 1992;Adinolfi et al, 1993Adinolfi et al, , 1995b. The presence of trophoblastic cells in the transcervical samples has been documented by the detection of chromosome Y-derived DNA sequences using fluorescent in situ hybridization (FISH) tests (Adinolfi et al, 1993) and of cellular elements reacting with monoclonal antibodies raised against placental antigens (Bulmer et al, 1995).…”

Section: Other Clinical Applicationsmentioning

confidence: 99%