Prenatal vitamin intake in first month of pregnancy and DNA methylation in cord blood and placenta in two prospective cohorts

Dou, John; Middleton, Lauren Y. M.; Zhou, Yong‐Gui; Benke, Kelly S.; Feinberg, Jason I.; Croen, Lisa; Hertz‐Picciotto, Irva; Newschaffer, Craig J.; LaSalle, Janine M.; Fallin, M. Daniele; Schmidt, Rebecca J.; Bakulski, Kelly M.

doi:10.1101/2022.03.04.22271903

Cited by 1 publication

(1 citation statement)

References 79 publications

(81 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In both studies, several sample- and probe-level quality control measures were applied, as described previously ( Bakulski et al, 2021 ; Dou et al, 2022 ). Samples were excluded if they were duplicates, had low overall array intensity, or a discrepancy between reported sex and empirically predicted sex.…”

Section: Methodsmentioning

confidence: 99%

Prenatal Exposure to Ambient Air Pollution and Epigenetic Aging at Birth in Newborns

Song

Feinberg²,

Bakulski³

et al. 2022

Front. Genet.

Self Cite

View full text Add to dashboard Cite

In utero air pollution exposure has been associated with adverse birth outcomes, yet effects of air pollutants on regulatory mechanisms in fetal growth and critical windows of vulnerability during pregnancy are not well understood. There is evidence that epigenetic alterations may contribute to these effects. DNA methylation (DNAm) based age estimators have been developed and studied extensively with health outcomes in recent years. Growing literature suggests environmental factors, such as air pollution and smoking, can influence epigenetic aging. However, little is known about the effect of prenatal air pollution exposure on epigenetic aging. In this study, we leveraged existing data on prenatal air pollution exposure and cord blood DNAm from 332 mother-child pairs in the Early Autism Risk Longitudinal Investigation (EARLI) and Markers of Autism Risk in Babies-Learning Early Signs (MARBLES), two pregnancy cohorts enrolling women who had a previous child diagnosed with autism spectrum disorder, to assess the relationship of prenatal exposure to air pollution and epigenetic aging at birth. DNAm age was computed using existing epigenetic clock algorithms for cord blood tissue—Knight and Bohlin. Epigenetic age acceleration was defined as the residual of regressing chronological gestational age on DNAm age, accounting for cell type proportions. Multivariable linear regression models and distributed lag models (DLMs), adjusting for child sex, maternal race/ethnicity, study sites, year of birth, maternal education, were completed. In the single-pollutant analysis, we observed exposure to PM2.5, PM10, and O3 during preconception period and pregnancy period were associated with decelerated epigenetic aging at birth. For example, pregnancy average PM10 exposure (per 10 unit increase) was associated with epigenetic age deceleration at birth (weeks) for both Knight and Bohlin clocks (β = −0.62, 95% CI: −1.17, −0.06; β = −0.32, 95% CI: −0.63, −0.01, respectively). Weekly DLMs revealed that increasing PM2.5 during the first trimester and second trimester were associated with decelerated epigenetic aging and that increasing PM10 during the preconception period was associated with decelerated epigenetic aging, using the Bohlin clock estimate. Prenatal ambient air pollution exposure, particularly in early and mid-pregnancy, was associated with decelerated epigenetic aging at birth.

show abstract

Section: Methodsmentioning

confidence: 99%