“…Mesenteric arteries of 3-month old CMO and DMO rats were split into 4 groups and incubated at 37°C during 24 h in 100 μL buffer containing Leibovitz medium with 10% fetal bovine serum, a mix of antibiotic-antimycotic solution (1%, 15240062, Gibco, Courtaboeuf, France), Dapi (5 μg/mL, Molecular Probes, Carlsab, USA) and either Alexa Fluor594/Cadaverine (10 μmol/L, A-30678, Invitrogen, Carlsab, USA), or (Aceti et al, 2012 ) Alexa Fluor594/Cadaverine + SNP ( 10 −3 mol/L), or (Bunt et al, 2005 ) Alexa Fluor594/Cadaverine + dithiothreitol (DTT, 2 mmol/L, 43816, Sigma, St Quentin Fallavier, France), or (Gomes and Gil, 2011 ) Alexa Fluor594/Cadaverine + DTT (2 mmol/L) + SNP (10 −3 mol/L). Vessels were mounted on Mowiol and imaged on a confocal microscope (Nikon Eclipse TE2000S).…”