Preoperative laparoscopy

Rk, Wadhwa; McKenzie, Ray; Wadhwa,

doi:10.1016/s0140-6736(75)91730-4

Cited by 4 publications

(1 citation statement)

References 10 publications

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“…It functions as a protective factor during glucose deprivation and performs a broad spectrum of cellular functions including the management of reactive oxygen species and mitochondrial-or intracellular trafficking. 24 Our proteome analysis identified GRP75 as a potential therapeutic target related to CD147 knock-down. The expression of GRP75, increased in the KB/V cell line compared to its sensitive KB counterpart, was downregulated by CD147 silencing, suggesting that GRP75 is one of the CD147-interacting proteins that lead to the expression of the MDR phenotype by oral SCC.…”

Section: Discussionmentioning

confidence: 91%

Proteome Analysis of Multidrug Resistance of Human Oral Squamous Carcinoma Cells Using CD147 Silencing

Kuang

Chen

et al. 2008

J. Proteome Res.

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There is a correlation between the multidrug-resistance (MDR) of cancer cells and their enhanced invasive or metastatic potential. We studied the expression of CD147, a plasma membrane glycoprotein that plays a key role in tumor metastasis by stimulating the production of matrix metalloproteinases (MMPs), in sensitive human oral squamous KB and MDR derivative KB/V cells. Reverse transcription-PCR and flow cytometric analysis revealed that KB/V cells expressed CD147 at significantly higher levels than their parental KB cells. Using stable RNA interference, we succeeded in establishing a CD147 knock-down KB/V cell line (KB/VsiCD147). MTT colorimetric assay showed an increase in the chemosensitivity to vincristine (VCR), all transretinoic acid (ATRA), taxol, and 5-fluorouracil (5-Fu) of KB/VsiCD147 cells. Proteome analysis of KB, KB/V, and KB/VsiCD147 cell lines identified 21 differently expressed proteins. The enhanced expression of representative active proteins, GRP75 and CyPA, was confirmed by Western blotting and RT-PCR. In addition, pretreatment of KB/V cells with a CyPA-binding immunosuppressive drug, cyclosporine A (CsA), enhanced their chemosensitivity to VCR and 5-Fu. We document an abundance of molecules that interact with CD147 in the MDR of human oral squamous carcinoma cells. Additional studies are needed to investigate these novel target proteins of CD147.

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Section: Discussionmentioning

confidence: 91%