Preovulatory follicular fluid during in vitro maturation decreases polyspermic fertilization of cumulus-intact porcine oocytes

Bijttebier, Jo; Soom, Ann Van; Meyer, Evelyne; Mateusen, Bart; Maes, Dominiek

doi:10.1016/j.theriogenology.2008.04.046

Cited by 33 publications

(29 citation statements)

References 45 publications

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“…Funahashi et al [14] pointed out that dbcAMP did not influence penetration and polyspermy rate, Somfai et al [13] found similar penetration rates but lower polyspermy rates, and Kim et al [15] measured lower polyspermy rates but higher penetration rates. Decreasing polyspermy rates after IVF of cumulusintact oocytes can be due to an improved cumulus expansion [16]. Hence, probably, cAMP levels are not only regulating nuclear and cytoplasmic maturation through acting via the oocyte but also influencing cumulus expansion.…”

Section: Introductionmentioning

confidence: 99%

Increasing the cAMP concentration during in vitro maturation of pig oocytes improves cumulus maturation and subsequent fertilization in vitro

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Increasing the cAMP concentration during in vitro maturation of pig oocytes improves cumulus maturation and subsequent fertilization in vitro

et al. 2015

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“…The IVM rate was evaluated as the percentage of oocytes with a nuclear morphology corresponding to metaphases II (MII), which was considered "mature" (Bijttebier et al 2008). Degenerate oocytes were discarded and the proportion of MII oocytes was calculated from the nondegenerate oocytes.…”

Section: Methodsmentioning

confidence: 99%

In vitro fertilization of porcine oocytes is affected by spermatic coincubation time

et al. 2016

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The aim was to study the effects of different gamete coincubation times on porcine in vitro fertilization (IVF), and to verify whether efficiency could be improved by reducing oocyte exposure time to spermatozoa during IVF. In groups of 50, a total of 508 immature cumulus-oocyte complexes (COCs) were matured in NCSU-37 medium. The COCs were cultured for 44 hours and then inseminated with in natura semen (2,000 spermatozoa/ oocyte). The sperm and oocytes were coincubated according to the following treatments (T): T1 = oocytes exposed to spermatozoa for one hour (173 oocytes), T2 = oocytes exposed to spermatozoa for two hours (170 oocytes), and T3 = oocytes exposed to spermatozoa for three hours (165 oocytes). After these coincubation periods, the oocytes were washed in fertilization medium (TALP medium) to remove spermatozoa not bound to the zona pellucida and cultured in another similar medium (containing no sperm). Eighteen to twenty hours after fertilization, the putative zygotes were stained in Hoechst-33342 to evaluate the IVF results. The penetration rate was higher (P<0.05) after two hours of coincubation time than it was for one or three hours. Furthermore, 68.60% of the ova coincubated with the spermatozoa for two hours were monospermic. The oocytes exposed to spermatozoa for one hour (T1) presented a higher (P<0.01) rate of polyspermy than those in T2 and T3. Fertilization performance (%) did not differ (P>0.05) between oocytes exposed to spermatozoa for one (T1) and three hours (T3). However, optimum (P=0.048) results were obtained after two hours of coincubation, when the rate of fertilization performance was 50.16±8.52%. The number of penetrated sperm per oocyte, as well as male pronucleus formation, did not differ (P>0.05) between the treatments evaluated. Under these assay conditions, especially in relation to the sperm concentration used, gamete coincubation for a period of two hours appears to be optimal for monospermy and fertilization performance. Thus, it is the optimal time period for obtaining a large number of pig embryos capable of normal development. (FIV) de suínos e se a eficiência dessa técnica poderia ser melhorada pela redução no período que os ovócitos são expostos aos espermatozoides durante a FIV. Um total de 508 (em grupos de 50) complexos cumulus-ovócito (COCs) imaturos foram maturados no meio NCSU-37. Os COCs foram cultivados por 40-44 horas e então inseminados com sêmen in natura (2.000 espermatozoides/ovócito). Os espermatozoides e ovócitos foram coincubados de acordo com os seguintes tratamentos (T): T1 = ovócitos expostos aos espermatozoides por uma hora (173 ovócitos); T2 = ovócitos expostos aos espermatozoides por duas horas (170 ovócitos) e T3 = ovócitos expostos aos espermatozoides por três horas (165 ovócitos). Após esses períodos de coincubação, os ovócitos foram lavados em meio de fertilização (meio TALP) para remoção dos espermazoides não ligados a zona pelúcida e cultivados em outro mesmo meio (não contendo espermatozoides). Após 18-20 horas de fertilização, os p...

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“…Evaluation of the nuclear maturation was classified by chronological meiosis changes such as germinal vesicle (GV), germinal vesicle breakdown (GVBD), metaphase I (MI), anaphase I (AI), telophase I (TI), or metaphase II (MII) (Shirazi & Sadeghi, 2007). The nuclear maturation rate was calculated based on the percentage of oocytes reached the stage of metaphase II (MII) (Bijttebier et al, 2008).…”

Section: Evaluation Of Nuclear Maturation Ratementioning

confidence: 99%

Insulin-Like Growth Factor-I Concentration in the Follicular Fluid of Bali Cattle and Its Role in the Oocyte Nuclear Maturation and Fertilization Rate

Hasbi¹,

Gustina²,

Karja³

et al. 2017

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Preovulatory follicular fluid during in vitro maturation decreases polyspermic fertilization of cumulus-intact porcine oocytes

Cited by 33 publications

References 45 publications

Increasing the cAMP concentration during in vitro maturation of pig oocytes improves cumulus maturation and subsequent fertilization in vitro

Increasing the cAMP concentration during in vitro maturation of pig oocytes improves cumulus maturation and subsequent fertilization in vitro

In vitro fertilization of porcine oocytes is affected by spermatic coincubation time

Insulin-Like Growth Factor-I Concentration in the Follicular Fluid of Bali Cattle and Its Role in the Oocyte Nuclear Maturation and Fertilization Rate

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