Preparation, analysis and antioxidant evaluation of the controlled product of polysaccharide from Mactra veneriformis by mild acid hydrolysis

Abstract

The polysaccharides from Mactra veneriformis (MVPS) were degraded by controlled mild acid hydrolysis to produce active oligosaccharides. MVPS can easily be hydrolyzed by H2SO4 or HCl. The hydrolyzing process was investigated in acid addition and reaction time by evaluating the producing content of reducing sugar. Hydrolysis with 1M HCl to MVPS can generate a time-depended behavior that is mild and controllable. HPLC analysis monitored the change of oligosaccharides composition in hydrolyzing. Total nine oligosaccharides are recognizable in the HPLC profile, and their content showed a regular transformation in hydrolysis. Those nine ingredients were identified as glucooligosaccharides with DP from 1 to 7 by MS analysis. Antioxidant activities of the typical hydrolyzates as well as MVPs were further tested in assays of DPPH and hydroxyl radicals scavenging, and reducing power. It was found that HCl hydrolyzate exhibited stronger antioxidant effects than MVPS and H2SO4 hydrolyzate due to its higher content of oligosaccharides.

Protective Actions of Acidic Hydrolysates of Polysaccharide Extracted From Mactra veneriformis Against Chemical-Induced Acute Liver Damage

“…In our previous research (Wang et al, 2016), we degraded MVPS by mild H 2 SO 4 or HCl hydrolysis and found that hydrolysates were extremely influenced by adding an amount of acid and by the reaction time. To avoid thorough hydrolysis and obtain more oligosaccharide content in the product, degradation with 0.5 h of HCl was adopted to process MVPS due to its controllability.…”

“…The product Ah-MVPS were correspondingly prepared by incomplete degradation of HCl, as reported in our previous study (Wang et al, 2016). In brief, about 2.0g MVPS was dissolved in 100 mL distilled water to prepare 20 mg/mL of solution.…”

“…The first result obtained was that the extracted polysaccharide (MVPS) could provide hepatoprotective benefits for CCl 4 -induced liverdamaged mice. One derivative, hydrolysates of MVPS from HCl reaction, were then verified to have more superior antioxidant activity in comparison with the maternal polysaccharide (Wang et al, 2016). It was finally acknowledged that HCl hydrolysis might modify the physicochemical characters of the polysaccharide and improve its biological activity.…”

The antioxidant capacity evaluation of polysaccharide hydrolyzates from pumpkin using Caenorhabditis elegans model

“…Compared with their initial polysaccharides, the produced low molecular mass fragment exhibited increasing bioactivities because of their higher bioavailability and easier absorbency in living organisms (Juśkiewicza, Zduńczyk, Jankowski, Król, & Milala, 2008). Moreover, the derived oligosaccharides might produce some novel physiological activity owing to their different physicochemical properties and molecular structures (Moura, Macagnan, & Silva, 2015; Wang, Wu, Ji, Xue, & Liu, 2016). But up to present, few researchers have focused on hydrolysis of PPe and the anti‐oxidative stress effect on their degrading products.…”

Antioxidant and anti-diabetic effects of Auricularia auricular polysaccharides and their degradation by artificial gastrointestinal digestion - Bioactivity of Auricularia auricular polysaccharides and their hydrolysates [pdf]

“…The biological and physicochemical properties of biomacromolecules are different from those of conventional drug substances, particularly with regard to their molecular weight, physicochemical stability, solubility, biological half-life, conformational stability, oral bioavailability, dose requirements, and administration (Wang et al, 2009;Xiong, 2016). Natural polysaccharides exhibiting their biological activities are concerned with the oligosaccharide derivatives that are produced by gastric acid or gastric enzymes in the digestive tract (Agbagla-Dohnani et al, 2012;Wang et al, 2016). Several studies in recent years have shown that the derived oligosaccharides produced by the acid or enzymatic hydrolysis of polysaccharides can be used as antioxidants or prebiotics (Daisy et al, 2015;Suphavadee et al, 2016;Xu et al, 2015).…”