Preparation and Application of ¹³C-Labeled myo-Inositol to Identify New Catabolic Products in Inositol Metabolism in Lactobacillus casei

Abstract

myo-Inositol (mI) is widely distributed in all domains of life and is important for several cellular functions, including bacterial survival. The enzymes responsible for the bacterial catabolism of mI, encoded in the iol operon, can vary from one organism to another, and these pathways have yet to be fully characterized. We previously identified a new scyllo-inositol dehydrogenase (sIDH) in the iol operon of Lactobacillus casei that can oxidize mI in addition to the natural substrate, scyllo-inositol, but the product of mI oxidation was not determined. Here we report the identification of these metabolites by monitoring the reaction with 13

Phosphonate and α-Fluorophosphonate Analogues of d-Glucose 6-Phosphate as Active-Site Probes of 1l-myo-Inositol 1-Phosphate Synthase

“…S. cerevisiae mIPS was expressed and purified, and the activity was confirmed, as reported previously.…”

“…All other chemicals were obtained from Sigma-Aldrich (Oakville, ON) unless otherwise described and were of reagent grade or higher. Deionized distilled water was purified using a Barnstead S. cerevisiae mIPS was expressed and purified, and the activity was confirmed, as reported 28 previously. 6-O-Trityl-D-glucopyranose.…”

“…Phosphonate inhibition was assessed as reported using a discontinuous method using a Biotek Synergy 4 plate reader. A master solution of mIPS (2 μM) was prepared containing 0.1 M MOPS (pH 7.0), 0.6 mM NAD + , and 10 mM NH 4 Cl.…”

Study of the molecular interaction of a phosphonium-based ionic liquid within myo-inositol and non-steroidal anti-inflammatory drugs

“…22,23 MI is a biologically active carbocyclic sugar that acts as a signal transducer in response to hormones, neurotransmitters, and growth factors. 24 MI is a member of the inositol family, which has also been called cyclitols and polyols. 25,26 It is a primary natural product that is abundant in nature.…”