PREPARATION AND ASSAY OF<scp>D</scp>-ERYTHROSE 4-PHOSPHATE

Baxter, J. N.; Perlin, Arthur S.; Simpson, F. J.

doi:10.1139/o59-022

Cited by 28 publications

(2 citation statements)

References 8 publications

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“…DAHP synthase was a gift from Dr. Klaus Herrmann. Erythrose 4-phosphate was synthesized as previously described (Baxter et al, 1959). Shikimate 3-phosphate was prepared by fermentation with Aerobacter aerogenes A170-40 (American Type Culture Collection 25597) as described by Knowles & Sprinson (1970).…”

Section: Methodsmentioning

confidence: 99%

Mechanism of enolpyruvylshikimate-3-phosphate synthase exchange of phosphoenolpyruvate with solvent protons

Anton¹,

Hedstrom²,

Fish³

et al. 1983

Biochemistry

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Enolpyruvyl shikimate-3-phosphate synthase (EC 2.5.1.19) was purified to near homogeneity (-90% purity) from Klebsiella pneumoniae. Glyphosate, a herbicide, competitively inhibits with phosphoenolpyruvate (PEP) with Ki =M. An analogue of shikimate 3-phosphate, 4,5-dideoxyshikimate 3-phosphate (ddS3P), competitively inhibits with shikimate 3-phosphate with K, = M. The enzyme is inactivated by 3-bromopyruvate. T I l 2 for inactivation is approximately 5 min at 0.15 mM 3-bromopyruvate. PEP protects against inactivation by 3-bromopyruvate, while ddS3P alone has no effect on the rate of inactivation. The protective effect of PEP is enhanced approximately 16-fold in the presence of ddS3P. The enzyme catalyzes exchange of solvent 3H into PEP in the presence of ddS3P but not in its absence.

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Section: Methodsmentioning

confidence: 99%

Mechanism of enolpyruvylshikimate-3-phosphate synthase exchange of phosphoenolpyruvate with solvent protons

Anton¹,

Hedstrom²,

Fish³

et al. 1983

Biochemistry

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“…For these reasons the status of free erythrose 4-phosphate monomer in liver and other tissues requires further investigation. It is of note that Dische & Igals (1961), Baxter et al (1959), Severin & Stepanova (1973) and Blackmore et al (1976) have all reported erratic and unexplainable behaviour of erythrose 4-phosphate in buffer and with different tissue extracts.…”

Section: Phosphotransferase-catalysed Reactionsmentioning

confidence: 95%

New reaction sequences for the non-oxidative pentose phosphate pathway

Williams¹,

Blackmore²,

Clark³

1978

Biochemical Journal

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1. Reactions leading to the formation of 14C-labelled volatile compounds and compounds volatile under acid conditions were investigated in a system actively synthesizing hexose 6-phosphates from [U-14C]ribose 5-phosphate by reactions catalysed by enzymes prepared from acetone-dried powder of rat liver; no reactions involving 14C-labelled volatile compounds were detected. Similarly the fixation of 14C-labelled volatile compounds into hexose 6-phosphate could not be detected. 2. A complete carbon balance was made for the reactants, intermediates and products of the reactions involved in the conversion of ribose 5-phosphate into hexose 6-phosphate by enzymes of rat liver. Five additional intermediates of pentose 5-phosphate metabolism in liver were detected, namely D-manno-heptulose 7-phosphate, D-altro-heptulose 1,7-bisphosphate, D-glycero-D-ido-octulose 1,8-bisphosphate, D-glycero-D-altro-octulose 1,8-bisphosphate and D-arabinose 5-phosphate. 3. D-Arabinose 5-phosphate was found to be utilized by a rat liver enzyme preparation to produce both hexose 6-phosphate and triose phosphate. 4. D-Arabinose 5-phosphate was reversibly converted into other pentose 5-phosphates. Paper chromatographic and enzymic evidence indicated that the conversion involved an enzyme tentatively named arabinose phosphate 2-epimerase, which catalyses the following reaction: D-arabinose 5-P in equilibrium D-ribose-5-P. 5. A variety of rat tissues also utilized D-arabinose 5-phosphate to produce both hexose 6-phosphate and triose phosphate and at a rate comparable with that obtained with D-ribose 5-phosphate. 6. A new reaction sequence for the non-oxidative pentose phosphate pathway in liver is proposed.

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