Preparation and assay of urogastrone, an inhibitor of gastric acid secretion

Abstract

Summary1. Urogastrone, an acid gastric secretion inhibitor extracted from human urine, was assayed in the anaesthetized rat by circulating through its stomach a succinic-propionic buffer and recording electrometrically the secretion of acid in response to carbachol, and inhibition of carbachol secretion by urogastrone. 2. Quantitative assay procedures are described requiring the administration of only one or two doses of urogastrone to each rat.3. An extraction procedure for urogastrone is described based on the use of zeocarb, a cationic polystyrene resin.4. The most purified samples contained 40-80 times the amount/milligram which was just detectable by the assay method.

Chemical and physical properties of the human urinary glycoprotein with gastric antisecretory activity

“…Inhibitors of gastric secretion have been found in human urine; these have been grouped under the general name of 'urinary gastric secretory depressant' or simply 'urogastrone' (Gray et al, 1940), where the term 'gastrone' generically indicates the factor inhibiting all forms of gastric acid stimulation (Thomson, 1966). Several authors (Friedman, 1951;Gregory, 1955;Mongar & Rosenoer, 1962;Morimoto & Yamamoto, 1969;Lawrence et al, 1971) described extraction procedures and partial chemical and physiological characterizations of purified antisecretory fractions. However, these studies did not lead to a clear elucidation of the chemical nature and physiological properties of the various isolated inhibitors.…”