Preparation and biocompatibility of a chitin nanofiber/gelatin composite film

Ogawa, Yoko; Aso, Kazuo; Izawa, Hironori; Morimoto, Minoru; Ochi, Kosuke; Osaki, Tomohiro; Ito, Norihiko; Okamoto, Yoshiharu; Saimoto, Hiroyuki; Ifuku, Shinsuke

doi:10.1016/j.ijbiomac.2017.02.041

Cited by 46 publications

(18 citation statements)

References 26 publications

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“…[136][137][138][139] Composite films of chitin nanofibers and gelatin have been prepared by casting and freeze-drying highly viscous precursor solutions. 140 The water content of chitin nanofiber-gelatin biomaterials may be precisely engineered by tailoring the gelatin content. 140 Increasing the gelatin concentration increased the swelling ratio.…”

Section: Gelatin-chitinmentioning

confidence: 99%

“…140 The water content of chitin nanofiber-gelatin biomaterials may be precisely engineered by tailoring the gelatin content. 140 Increasing the gelatin concentration increased the swelling ratio. The nanofiber-gelatin films did not induce inflammation and strongly promoted fibroblast proliferation, indicating high biocompatibility and bioactivity.…”

Section: Gelatin-chitinmentioning

confidence: 99%

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Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Afewerki

Sheikhi

Kannan

et al. 2018

Bioengineering & Transla Med

309

210

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Gelatin is a promising material as scaffold with therapeutic and regenerative characteristics due to its chemical similarities to the extracellular matrix (ECM) in the native tissues, biocompatibility, biodegradability, low antigenicity, cost‐effectiveness, abundance, and accessible functional groups that allow facile chemical modifications with other biomaterials or biomolecules. Despite the advantages of gelatin, poor mechanical properties, sensitivity to enzymatic degradation, high viscosity, and reduced solubility in concentrated aqueous media have limited its applications and encouraged the development of gelatin‐based composite hydrogels. The drawbacks of gelatin may be surmounted by synergistically combining it with a wide range of polysaccharides. The addition of polysaccharides to gelatin is advantageous in mimicking the ECM, which largely contains proteoglycans or glycoproteins. Moreover, gelatin–polysaccharide biomaterials benefit from mechanical resilience, high stability, low thermal expansion, improved hydrophilicity, biocompatibility, antimicrobial and anti‐inflammatory properties, and wound healing potential. Here, we discuss how combining gelatin and polysaccharides provides a promising approach for developing superior therapeutic biomaterials. We review gelatin–polysaccharides scaffolds and their applications in cell culture and tissue engineering, providing an outlook for the future of this family of biomaterials as advanced natural therapeutics.

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Section: Gelatin-chitinmentioning

confidence: 99%

Section: Gelatin-chitinmentioning

confidence: 99%

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Afewerki

Sheikhi

Kannan

et al. 2018

Bioengineering & Transla Med

309

210

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“…For instance, chitosan can be degraded in vertebrates mainly by lysozyme and certain bacterial enzymes in the colon [22]. Moreover, chitosan presents a high in vivo biocompatibility and biodegradability with minimal inflammation and body reaction when applied as tissue [23]. Moreover, the film adhesion on tissue can induce the proliferation of fibroblasts, indicating high biocompatibility [23].…”

Section: Chitin and Chitosan Overviewmentioning

confidence: 99%

“…Moreover, chitosan presents a high in vivo biocompatibility and biodegradability with minimal inflammation and body reaction when applied as tissue [23]. Moreover, the film adhesion on tissue can induce the proliferation of fibroblasts, indicating high biocompatibility [23]. Despite the great biological properties, such as biocompatibility, nonimmunogenicity, antibacterial, and anti-tumorigenicity, there is a lack of sufficient knowledge on the regenerative mechanism of this biopolymer [24].…”

Section: Chitin and Chitosan Overviewmentioning

confidence: 99%

Biodegradable Chitosan Matrix Composite Reinforced with Titanium Dioxide for Biocidal Applications

Calero¹,

Berríos²,

Suárez³

2019

Renewable and Sustainable Composites

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Access to drinkable water is increasingly difficult in developing nations. According to the World Health Organization, more than 1.2 billion people lack access to this resource. The scientific community has risen to the challenge by developing innovating methods to purify water. In this context, a chitosan/titanium dioxide biocomposite represents an appealing, economically viable solution to remove oils, heavy metals, and pathogen colonies from contaminated waters. The present chapter reports the study of a chitosan film matrix impregnated with nanoparticles, which was capable of expressing antibacterial properties when exposed to UV light. Several techniques allowed characterizing the biocomposite uniformity, corroborating the crystal structure and assessing the bonding type of this biocomposite mixture. Antibacterial tests with Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) were completed via growth curve analysis and the Kirby-Bauer technique. The results of this encompassing study revealed that bacterial growth was reduced by more than 50%. Optical microscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction helped to characterize this biocomposite film. This chapter discusses how a novel and biodegradable film represents a better antibacterial material that is able to eliminate pathogens cost-effectively.

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“…In vivo evaluation was performed according to our previous method with slight modifications [32]. A schematic illustration of the in vivo test is shown in Figure 6a.…”

Section: In Vivo Evaluationmentioning

confidence: 99%

Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds

Izawa

Okuda

Yonemura

et al. 2018

Colloids and Interfaces

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Microscopic surface architectures that can be easily manufactured have been in demand as mechano-structural cues for tissue engineering. Microscopic surface reliefs synthesized by wrinkling were expected as cell culture scaffolds for cell proliferation, control of cellular alignment and differentiation, and spheroid generation. We previously developed bio-based wrinkled films prepared via lignification-mimetic reactions and drying. Although these films are expected as a candidate for cell culture scaffolds, stability and morphology of the wrinkled surfaces in aqueous buffer solutions were not explored. Here, we investigate the surface morphologies of the wrinkled films in phosphate-buffered saline, and their application to 3T3 cell culture. The wrinkled film prepared with the immersion treatment at 40 • C maintained its wrinkled structure in phosphate-buffered saline even after five days, although the wrinkles were broadened by hydration of the skin layer. Interestingly, higher cell numbers were observed in the 3T3 cell culture using the wrinkled film than using flat film with the same surface composition. In addition, the high biocompatibility of the wrinkled film was confirmed by in vivo experiments. These results strongly encourage application of the wrinkled film as a mechano-structural cue. Studies of the advanced applications for the wrinkled films are now in progress.

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Preparation and biocompatibility of a chitin nanofiber/gelatin composite film

Cited by 46 publications

References 26 publications

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Biodegradable Chitosan Matrix Composite Reinforced with Titanium Dioxide for Biocidal Applications

Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds

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