Preparation and Biological Application of Rare Earth Upconversion Fluorescent Nanomaterials

Shan, Shuang; Wu, Hao; Tan, Mingqian; Ma, Xiaojun

doi:10.3724/sp.j.1206.2013.00254

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…For example, the synthesis of NaGdF 4 ∶Yb 3þ , Er 3þ nanoparticles have some drawbacks for biomedical applications; therefore, gold or silica need to be used to render them with biocompatible properties. [37][38][39] In addition, one of the problems with carbon nanoparticles is the fact that they need to be excited with near-UV light, which can damage tissues around the cancer cells. 34 Rare earth doped zirconia (ZrO 2 ) nanophosphors present efficient emission in the visible region when they are under IR excitation.…”

Section: Introductionmentioning

confidence: 99%

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

et al. 2015

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Abstract. This work reports the synthesis, structural characterization, and optical properties of ZrO 2 ∶Yb 3þ -Er 3þ (2-1 mol%) nanocrystals. The nanoparticles were coated with 3-aminopropyl triethoxysilane (APTES) and further modified with biomolecules, such as Biotin-Anti-rabbit (mouse IgG) and rabbit antibody-AntiKi-67, through a conjugation method. The conjugation was successfully confirmed by Fourier transform infrared, zeta potential, and dynamic light scattering. The internalization of the conjugated nanoparticles in human cervical cancer (HeLa) cells was followed by two-photon confocal microscopy. The ZrO 2 ∶Yb 3þ -Er 3þ nanocrystals exhibited strong red emission under 970-nm excitation. Moreover, the luminescence change due to the addition of APTES molecules and biomolecules on the nanocrystals was also studied. These results demonstrate that ZrO 2 ∶Yb 3þ -Er 3þ nanocrystals can be successfully functionalized with biomolecules to develop platforms for biolabeling and bioimaging. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Introductionmentioning

confidence: 99%

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

et al. 2015

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“…Compared to semiconductor quantum dots (QDs), [ 6 ] rare earth doped NPs show not only good chemical stability but also less heavy metal toxicity, and avoid the excitation of ultraviolet light sources, which not only has weak penetration ability but also cause great damage to biological tissues. [ 7,8 ]…”

Section: Introductionmentioning

confidence: 99%

In vivo immunotoxicity of Gd₂O₃:Eu³⁺ nanoparticles and the associated molecular mechanism

Zheng

Tian

Cai

et al. 2020

J Biochem & Molecular Tox

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The in vivo toxicity of Gd2O3:Eu3+ nanoparticles (NPs) used as dual‐modal nanoprobes for molecular imaging has not been studied, and the corresponding molecular mechanism of immunotoxicity remains unknown. In this study, we investigated the cytotoxicity, in vitro apoptosis, and in vivo immunotoxicity of Gd2O3:Eu3+ NPs. The NPs showed little immunotoxicity to BALB/c mice. We explored the possible role of the phosphoinositide 3‐kinase (PI3K) signaling pathway and found that reactive oxygen species could act as secondary messengers in cellular signaling, inhibiting PI3K expression in the liver. The immune suppression caused by PI3K inhibition helped the mice adapt to stress. The immunotoxicities caused by Gd2O3:Eu3+ and gadodiamide, a commonly used contrast agent, were not significantly different, and the mice were able to tolerate the immunotoxicity caused Gd2O3:Eu3+ NPs in vitro and in vivo experiments. The results suggest that Gd2O3:Eu3+ NPs are sufficiently biocompatible to be used safely in preclinical applications and show promise as bio‐imaging agents. Moreover, the in vivo molecular mechanism of immunotoxicity caused by the Gd2O3:Eu3+ NPs provides a platform for further research on the immunotoxicity of nano‐sized biomaterials.

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Preparation and Biological Application of Rare Earth Upconversion Fluorescent Nanomaterials

Cited by 2 publications

References 11 publications

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

In vivo immunotoxicity of Gd₂O₃:Eu³⁺ nanoparticles and the associated molecular mechanism

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Preparation and Biological Application of Rare Earth Upconversion Fluorescent Nanomaterials

Cited by 2 publications

References 11 publications

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

In vivo immunotoxicity of Gd2O3:Eu3+ nanoparticles and the associated molecular mechanism

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In vivo immunotoxicity of Gd₂O₃:Eu³⁺ nanoparticles and the associated molecular mechanism