Preparation and characteristics of hybrid scaffolds composed of β-chitin and collagen

Lee, Sang Bong; Kim, Yong Han; Chong, Moo Sang; Lee, Young Moo

doi:10.1016/j.biomaterials.2003.09.016

Cited by 95 publications

(67 citation statements)

References 18 publications

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“…Although genipin was involved in the crosslinking process, the HA-treated chitin-chitosan scaffolds could be efficiently biodegraded by BKCs, demonstrating their feasibility for chondrocyte cultures. For lysozymeassisted hydrolysis of chitin sacffolds, the weight remaining was as good as 70%-85% and became flattened over 6-day biodegradation (Lee et al, 2004). The remnant weight of 40%-70% can be a qualified degradation rate for a scaffold over 1-month in vitro degradation because chondrocytes require the protection of the material matrix from developing scattered cartilaginous tissues.…”

Section: Discussionmentioning

confidence: 95%

“…Chitin possesses not only the excellent wound healing, antibacterial, and anti-inflammatory abilities, but also the bioactive functions of enzyme immobilization on the amino groups. These properties were beneficial to the biomedical application of the chitin-containing tissue-engineering scaffolds (Hirano, 1999;Lee et al, 2004). Deposition of apatite on chitin scaffolds could further promote the mechanical and cell-induced properties; however, the pore size and pore shape would be nonuniformly distributed (Wan et al, 1998).…”

Section: Introductionmentioning

confidence: 96%

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Effect of genipin‐crosslinked chitin‐chitosan scaffolds with hydroxyapatite modifications on the cultivation of bovine knee chondrocytes

Kuo

Lin

2006

Biotech & Bioengineering

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Chitin and chitosan were hybridized in various weight percentages by genipin crosslinkage under various prefreezing temperatures to form tissue-engineering scaffolds via lyophilization. In addition, deposition of hydroxyapatite (HA) on the surface of the porous scaffolds was performed by precipitation method to achieve modified chemical compositions for chondrocyte attachments and growths. The experimental results revealed that a lower prefreezing temperature or a higher weight percentage of chitin in the chitin-chitosan scaffolds would yield a smaller pore diameter, a greater porosity, a larger specific surface area, a higher Young's modulus, and a lower extensibility. Moreover, a higher chitin percentage could also result in a higher content of amine groups after crosslink and a lower onset temperature for the phase transition after thermal treatment. A decrease in the prefreezing temperature from -4 degrees C to -80 degrees C, an increase in the chitin percentage from 20% to 50%, and an increase in the cycle number of alternate immersion for HA deposition from 1 to 5 generated positive effects on the cell number, the content of glycosaminoglycans, and the collagen level over 28-day cultivation of bovine knee chondrocytes.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 96%

Effect of genipin‐crosslinked chitin‐chitosan scaffolds with hydroxyapatite modifications on the cultivation of bovine knee chondrocytes

Kuo

Lin

2006

Biotech & Bioengineering

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“…In turn, the collagen is known to be an adequate substrate for adhesion of a majority of anchorage-dependent animal cells. 23 As for choosing agarose to be the polymeric basis of the scaffolds (A) to (C), this polysaccharide is chemically stable under the physiological conditions of animal organisms, where the agarose-lytic enzymes are absent, therefore such biopolymer is well suitable for the long-term functioning in the composition of potential BAP.…”

Section: Preparation Structure and Properties Of Cryogel Scaffoldsmentioning

confidence: 99%

Functional activity of insulinoma cells (INS‐1E) and pancreatic islets cultured in agarose cryogel sponges

Bloch

Lozinsky

Galaev

et al. 2005

J Biomedical Materials Res

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Here, we describe the preparation, structure, and properties of cryogel sponges, which represent a new type of macroporous biomaterial for tissue engineering. Cryogels were produced through freeze-thawing techniques, either from agarose alone or from agarose with grafted gelatin. The aim of this study was to evaluate agarose cryogel sponges as scaffolds for culturing both isolated pancreatic islets and insulinoma cells (INS-1E). In order to evaluate the effect of cell entrapment in artificial scaffolds, cell function reflected by insulin secretion and content was studied in cells cultivated for a 2-week period either in culture plastic plates or in cryogel sponge disks. Our results show that tumor-derived INS-1E cells grown either on plastic or on cryogels do not differ in their proliferation, morphology, insulin release, and intracellular insulin content. However, isolated pancreatic islets cultivated on cryogels sponge show 15-fold higher basal insulin secretion at 3.0 mM glucose than islets cultivated on plastic plates and fail to respond to stimulation with 16.7 mM glucose. In addition, these islets have about 2-fold lower insulin content compared to those grown in plastic plates. It is possible that the cell dysfunction noted in these in vitro experiments is due to the effect of the limited oxygen supply to the islets cultivated in cryogel sponge. Further in vivo studies are needed to clarify the nature of such an observation since according to previous reports, agarose and gelatin induce new vessel formation supporting enhanced oxygen supply.

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“…It is postulated that strong attraction between positive charges on the chitosan surface and negative charges on the cell surface enhanced the metabolic activity of cells on chitosan sponges (Mi et al, 2001). In vivo, chitosan enhances angiogenesis and wound healing, and supports growth and differentiation of osteoblasts (Lee et al, 2004).…”

Section: In-vitro Applicationmentioning

confidence: 99%

Biocomposite Materials

Khaled¹

2012

Composites and Their Applications

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Preparation and characteristics of hybrid scaffolds composed of β-chitin and collagen

Cited by 95 publications

References 18 publications

Effect of genipin‐crosslinked chitin‐chitosan scaffolds with hydroxyapatite modifications on the cultivation of bovine knee chondrocytes

Effect of genipin‐crosslinked chitin‐chitosan scaffolds with hydroxyapatite modifications on the cultivation of bovine knee chondrocytes

Functional activity of insulinoma cells (INS‐1E) and pancreatic islets cultured in agarose cryogel sponges

Biocomposite Materials

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