Preparation and characterization of a gallium-loaded antimicrobial artificial dermal scaffold

Xu, Zhaorong; Chen, Xiao; Tan, Rongwei; She, Zhigang; Chen, Zhaohong; Xia, Zhaofan

doi:10.1016/j.msec.2019.110063

Cited by 22 publications

(20 citation statements)

References 39 publications

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“…Antimicrobial activity of Ga has been reported [ 73 , 74 ], which could be of use for the treatment of infected wounds. A biocompatible, gallium-loaded, antimicrobial, artificial dermal scaffold has been recently proposed [ 75 ]. Other biomedical uses of Ga have also been previously reported due to its low toxicity [ 76 , 77 , 78 , 79 , 80 , 81 ].…”

Section: Discussionmentioning

confidence: 99%

Correlation between Elemental Composition/Mobility and Skin Cell Proliferation of Fibrous Nanoclay/Spring Water Hydrogels

et al. 2020

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Inorganic hydrogels formulated with spring waters and clay minerals are used to treat musculoskeletal disorders and skin affections. Their underlying mechanism of action for skin disorders is not clear, although it is usually ascribed to the chemical composition of the formulation. The aim of this study was to assess the composition and in vitro release of elements with potential wound healing effects from hydrogels prepared with two nanoclays and natural spring water. In vitro Franz cell studies were used and the element concentration was measured by inductively coupled plasma techniques. Biocompatibility studies were used to evaluate the potential toxicity of the formulation against fibroblasts. The studied hydrogels released elements with known therapeutic interest in wound healing. The released ratios of some elements, such as Mg:Ca or Zn:Ca, played a significant role in the final therapeutic activity of the formulation. In particular, the proliferative activity of fibroblasts was ascribed to the release of Mn and the Zn:Ca ratio. Moreover, the importance of formulative studies is highlighted, since it is the optimal combination of the correct ingredients that makes a formulation effective.

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Section: Discussionmentioning

confidence: 99%

Correlation between Elemental Composition/Mobility and Skin Cell Proliferation of Fibrous Nanoclay/Spring Water Hydrogels

et al. 2020

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“…SEM micrographs (Fig. 3b) show that these fabricated CSSs presented a porous structure with pore sizes ranging from 50 to 150 μm and a continuous radial structure, which is beneficial for nutrient transport, cell migration, and angiogenesis [48]. There was no significant difference between the CSSs and Lando® Artificial Dermal Regeneration Matrix products.…”

Section: Csss Desired Were Successfully Fabricatedmentioning

confidence: 95%

“…Based on the existing protocol [48], the surface of the differentiated hASCs on the polyester membrane and cell-seeded CSSs was also observed via SEM. After the culture medium was decanted, the samples were rinsed with PBS twice, fixed in 2.5% glutaraldehyde for 2 h, followed by deionized water to rinse again.…”

Section: Micromorphology Via Semmentioning

confidence: 99%

Epithelial differentiation of human adipose-derived stem cells (hASCs) undergoing three-dimensional (3D) cultivation with collagen sponge scaffold (CSS) via an indirect co-culture strategy

Gao

et al. 2020

Stem Cell Res Ther

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Background: Three-dimensional (3D) cultivation with biomaterials was proposed to facilitate stem cell epithelial differentiation for wound healing. However, whether human adipose-derived stem cells (hASCs) on collagen sponge scaffold (CSS) better differentiate to keratinocytes remains unclear. Methods: 3D cultivation with CSS on hASC epidermal differentiation co-cultured with HaCaT cells at air-liquid interface (ALI) was compared with two-dimensional (2D) form and cultivation without "co-culture" or "ALI." Cellular morphology, cell adhesion, and growth condition were evaluated, followed by the protein and gene expression of keratin 14 (K14, keratinocyte specific marker). Results: Typical cobblestone morphology of keratinocytes was remarkably observed in co-cultured hASCs at ALI, but those seeded on the CSS exhibited more keratinocyte-like cells under an invert microscope and scanning electron microscope. Desired cell adhesion and proliferation were confirmed in 3D differentiation groups by rhodamine-labeled phalloidin staining, consistent with H&E staining. Compared with those cultured in 2D culture system or without "ALI," immunofluorescence staining and gene expression analysis revealed hASCs co-cultured over CSS expressed K14 at higher levels at day 15. Conclusions: CSS is positive to promote epithelial differentiation of hASCs, which will foster a deeper understanding of artificial dermis in skin wound healing and regeneration.

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“…Hydrogel scaffolds composed of collagen, alginate, or a combination of hyaluronic acid (HA) and corn silk extract (CSE) were loaded with gallium ions or silver particles, and cell viability and antimicrobial capability were assessed. For the collagen-gallium scaffold, a concentration of 0.025% gallium had an antibacterial potency of > 90% against S. aureus and P aeruginosa and a slight adverse impact on cell proliferation in vitro compared with the control group, while in vivo tests in rats demonstrated a marked decrease in bacterial CFUs over a 7-day duration compared with the control [105]. For the injectable collagen and alginate gels loaded with either silver salt formulation lactate or saccharinate, in vitro results demonstrated that both collagen-silver scaffolds and alginatesilver scaffolds were effective against S. epidermidis and P. aeruginosa bacteria compared with control, though alginate-silver scaffolds were far more dose dependent.…”

Section: Antibacterial Scaffoldsmentioning

confidence: 97%

“…An array of antimicrobial scaffolds has been recently devised which demonstrate wound healing capabilities while preventing the development of biofilms and infection at the wound site [102][103][104][105]. A number of materials have been employed for use as scaffolding components, which exhibit high levels of biocompatibility and incorporate metals and proteins to prevent the proliferation of various species of bacteria.…”

Section: Antibacterial Scaffoldsmentioning

confidence: 99%

Development of Novel Microenvironments for Promoting Enhanced Wound Healing

Scull

Brown

2020

Curr. Tissue Microenviron. Rep.

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Purpose of Review Non-healing wounds are a significant issue facing the healthcare industry. Materials that modulate the wound microenvironment have the potential to improve healing outcomes. Recent Findings A variety of acellular and cellular scaffolds have been developed for regulating the wound microenvironment, including materials for controlled release of antimicrobials and growth factors, materials with inherent immunomodulative properties, and novel colloidal-based scaffolds. Scaffold construction methods include electrospinning, 3D printing, decellularization of the extracellular matrix, or a combination of techniques. Material application methods include layering or injecting at the wound site. Summary Though these techniques show promise for repairing wounds, all material strategies thus far struggle to induce regeneration of features such as sweat glands and hair follicles. Nonetheless, innovative technologies currently in the research phase may facilitate future attainment of these features. Novel methods and materials are constantly arising for the development of microenvironments for enhanced wound healing. Keywords Wound microenvironment. Wound modulation. Chronic wounds. Biomaterials. Biomaterials for wound healing. Novel wound healing materials This article is part of the Topical Collection on Cell Behavior Manipulation

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Preparation and characterization of a gallium-loaded antimicrobial artificial dermal scaffold

Cited by 22 publications

References 39 publications

Correlation between Elemental Composition/Mobility and Skin Cell Proliferation of Fibrous Nanoclay/Spring Water Hydrogels

Correlation between Elemental Composition/Mobility and Skin Cell Proliferation of Fibrous Nanoclay/Spring Water Hydrogels

Epithelial differentiation of human adipose-derived stem cells (hASCs) undergoing three-dimensional (3D) cultivation with collagen sponge scaffold (CSS) via an indirect co-culture strategy

Development of Novel Microenvironments for Promoting Enhanced Wound Healing

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