Preparation and characterization of an immunoaffinity column for the selective extraction of aflatoxin B1 in 13 kinds of foodstuffs

Xie, Jun; Peng, Tao; He, Jianli; Yu, Songlin; Fan, Chun‐Lin; Chen, Ying; Jiang, Wenxiao; Chen, Min; Wang, Qi; Pei, Xingyao; Ding, Shuangyang; Jiang, Haiyang

doi:10.1016/j.jchromb.2015.06.022

Cited by 26 publications

(16 citation statements)

References 38 publications

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“…To immobilize Abs on a solid sorbent, the most common approach is with regard to their covalent bonding, which is often achieved by coupling an accessible amino group of the Abs with a support that contains reactive groups such as epoxy [93] or aldehyde [97] or groups that can be activated using glutardialdehyde [71,100,101], carbonyldiimidazole, cyanogen bromide (CNBr) or N-hydrosuccinimide (NHS). Some activated supports are commercially available such as NHS- [70] or CNBr- [69,76,[84][85][86][87][88]91,[94][95][96]103,112] activated Sepharose or glutardialdehyde activated silica [74,77,82]. Non-covalent binding can also be used to couple Abs to the sorbent.…”

Section: Antibody Production and Development Of Immunosorbentsmentioning

confidence: 99%

Immunoaffinity Extraction and Alternative Approaches for the Analysis of Toxins in Environmental, Food or Biological Matrices

Delaunay

Combès

Pichon

2020

Toxins

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The evolution of instrumentation in terms of separation and detection allowed a real improvement of the sensitivity and analysis time. However, the analysis of ultra-traces of toxins in complex samples requires often a step of purification and even preconcentration before their chromatographic analysis. Therefore, immunoaffinity sorbents based on specific antibodies thus providing a molecular recognition mechanism appear as powerful tools for the selective extraction of a target molecule and its structural analogs to obtain more reliable and sensitive quantitative analysis in environmental, food or biological matrices. This review focuses on immunosorbents that have proven their efficiency in selectively extracting various types of toxins of various sizes (from small mycotoxins to large proteins) and physicochemical properties. Immunosorbents are now commercially available, and their use has been validated for numerous applications. The wide variety of samples to be analyzed, as well as extraction conditions and their impact on extraction yields, is discussed. In addition, their potential for purification and thus suppression of matrix effects, responsible for quantification problems especially in mass spectrometry, is presented. Due to their similar properties, molecularly imprinted polymers and aptamer-based sorbents that appear to be an interesting alternative to antibodies are also briefly addressed by comparing their potential with that of immunosorbents.

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Section: Antibody Production and Development Of Immunosorbentsmentioning

confidence: 99%

Immunoaffinity Extraction and Alternative Approaches for the Analysis of Toxins in Environmental, Food or Biological Matrices

Delaunay

Combès

Pichon

2020

Toxins

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“…A utilização desta técnica, no entanto, apresenta algumas desvantagens, como retenção não específica e gastos provocados pela necessidade de uso de uma grande quantidade de solventes orgânicos, fatos que podem comprometer a recuperação e a sensibilidade para as micotoxinas alvos das análises que serão realizadas (Xie et al, 2015).…”

Section: Preparação Eficaz Da Amostra Para Análise De Micotoxinasunclassified

Procedimentos para colheita de amostras e análises laboratoriais de micotoxinas presentes em dietas humanas e animais

Savi¹,

Pereira

Frozza³

et al. 2017

Pubvet

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RESUMO.Micotoxinas são substâncias tóxicas produzidas pelo metabolismo secundário de fungos filamentosos e capazes de desencadear uma série de problemas relacionados à produção agropecuária e à saúde pública. O monitoramento destes agentes tóxicos é necessário, devendo-se levar em consideração para realização de tal atividade a diversidade das amostras que são colhidas, os tipos distintos de micotoxinas, bem como a distribuição não uniforme destes contaminantes. A escolha adequada dos pontos de amostragem, bem como do uso de uma metodologia eficaz que considere os possíveis interferentes e contemple os níveis de detecção esperados também são fatores indispensáveis para o sucesso e confiabilidade das análises realizadas. Com o intuito de atender às exigências da legislação brasileira para micotoxinas, vários alimentos deverão ser analisados, para isso, o conhecimento de procedimentos para colheita de amostras e análises laboratoriais são importantes para pequenos produtores e para indústria de alimentos. Considerando que a aplicação adequada destas metodologias não apenas pode aumentar a produção agrícola e sua consequente lucratividade, como também propiciar a segurança alimentar dos diversos produtos brasileiros destinados às dietas humanas e/ou animais, esta revisão visa descrever as principais metodologias existentes no Brasil para colheita de amostras e análise de micotoxinas, assim como abordar os principais métodos analíticos existentes para detecção e quantificação destes contaminantes. Palavras chave: Alimentos, amostragem, análises laboratoriais, metodologias analíticasProcedures for sampling and laboratory tests of mycotoxins existing in human and animals feed ABSTRACT. Mycotoxins are toxic substances produced by the secondary metabolism of filamentous fungi and able to trigger series of problems related to agricultural production and public health. Thereby, monitoring of toxic agents is absolutely necessary and must be taken into account for the performance of such activity the diversity of samples to be collected, the different types of mycotoxins as well as the non-uniform distribution of these contaminants. Proper selection of sampling process as well as the use of a effective methodology will eliminate the trace of interference in order to have a successful analyses. Thus, in order to meet the requirements of the current Brazilian legislation for mycotoxins, various foods should be analyzed for this, knowledge of procedures for sampling and laboratory analyzes are of fundamental importance to both small producers and for the food

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“…IAC kolone se čuvaju na sobnoj temperaturi, međutim vreme upotrebe je ograničeno na 12 meseci od proizvodnje, a cena im je visoka što može predstavljati ograničavajući faktor za njihovu primenu. Metoda određivanja aflatoksina i ohratoksina uz primenu IAC kolona je specifična, precizna i osetljiva, a upravo primena IAC kolona čini metodu jednostavnom za izolovanje aflatoksina i ohratoksina, što i drugi autori navode kao jednu od najvećih prednosti tehnika za pripremu uzorka koje se zasnivaju na principu imunoafinitetne hromatografije (6,11,12). Prema zahtevima SRPS CEN/TR 16059:2012, ispitivana metoda ispunjava zahteve pouzdanosti, primenljivosti i nesigurnosti merenja, ipak, rezultati upućuju na potrebu za daljom optimizacijom (10).…”

Section: Rezultati I Diskusijaunclassified

Application of immunoaffinity columns for different food item samples preparation in micotoxins determination

Curcic¹,

Ljubičić²,

Vuković³

et al. 2016

Arh farmaciju

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2Zavod za javno zdravlje Sremska Mitrovica, Stari šor 47, Sremska Mitrovica 3 Gradski Zavod za javno zdravlje Beograd, Bulevar despota Stefana 54a, Beograd *Autor za korespondenciju: Marijana Ćurčić, tel: +381113951248; e-mail: makitox@pharmacy.bg.ac.rs Kratak sadržajU analitičkim metodama koje se koriste u monitoringu posebna pažnja se pridaje pripremi uzorka, pa je i cilj ovog rada ispitivanje efikasnosti primene imunoafinitetnih kolona (IAC) za pripremu uzoraka kod određivanja aflatoksina i ohratoksina koje se zasnivaju na principu tečno-čvrste ekstrakcije. Koncentracija aflatoksina i ohratoksina određivana je u ukupno 56 uzoraka različitih namirnica: pšenica, kukuruz, pirinač, ječam i ostala žita (19 uzoraka), brašno i proizvodi od brašna od žita i dodaci za pekarsku industriju (7 uzoraka), voće i povrće (3 uzorka), lešnik, orah, badem, brašno od kokosa (4 uzorka), prženi kakaovac, kikiriki, čajevi, kafa (16 uzoraka), začini (4 uzorka) i meso i proizvodi od mesa (4 uzorka). Dobijeni rezultati ukazuju na prednost primene IAC kolona u pripremi uzoraka, prvenstveno je postignuto povećanje specifičnosti metode usled vezivanja ekstrahovanih analita za inkorporirana specifična antitela i ispiranje ostalih komponenti uzorka, koje mogu ometati dalju analizu. Prednost je generisanje male količine otpadnih organskih rastvarača i smanjenje izloženosti osoblja koje izvodi metodu hromatografskog određivanja mikotoksina. Posebno je od značaja postignuto povećanje osetljivosti metode jer je postignuto da kvantifikacioni limiti za određivanje aflatoksina i ohratoksina budu znatno niži od maksimalno dozvoljenih koncentracija ovih jedinjenja propisanih nacionalnim pravilnikom.

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Preparation and characterization of an immunoaffinity column for the selective extraction of aflatoxin B1 in 13 kinds of foodstuffs

Cited by 26 publications

References 38 publications

Immunoaffinity Extraction and Alternative Approaches for the Analysis of Toxins in Environmental, Food or Biological Matrices

Immunoaffinity Extraction and Alternative Approaches for the Analysis of Toxins in Environmental, Food or Biological Matrices

Procedimentos para colheita de amostras e análises laboratoriais de micotoxinas presentes em dietas humanas e animais

Application of immunoaffinity columns for different food item samples preparation in micotoxins determination

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