Preparation and characterization of antifungal colloidal copper nanoparticles and their antifungal activity against Fusarium oxysporum and Phytophthora capsici

Abstract

Copper nanoparticles (CuNPs) possess a promising antibacterial and antifungal activity with low-cost reagent and fabrication. In this study, we report the antifungal activity of pure CuNPs against Fusarium oxysporum (F. oxysporum) and Phytophthora capsici (P. capsici). CuNPs were prepared by reducing Cu 2þ from copper(II) chloride dihydrate via a green chemical reduction agent using ascorbic acid as both reducing agent and antioxidant agent in aqueous media and polyvinylpyrrolidone as a stabilizer. The effect of the solution pH, reduction time, ascorbic-acid-to-Cu 2þ molar ratio, role of stabilizing agent polyvinylpyrrolidone to particle size of CuNPs were studied. The antifungal activity of CuNPs at different concentrations and different particle sizes against two plant pathogenic fungi F. oxysporum and P. capsici has been tested by the agar disc diffusion technique. Characterizations were carried out by X-ray diffraction measurements, transmission electron microscopy, and ultravioletevisible light, which revealed that the CuNPs obtained at different synthesis conditions have different particles size, resulting in different antifungal activities. The synthesized CuNPs demonstrated significant antifungal activity against F. oxysporum and P. capsici. F. oxysporum and P. capsici were entirely inhibited at the concentration of CuNPs of 30 ppm after 3 days of incubation and 7.5 ppm after 1 day of incubation, respectively. Our results suggest that synthesized CuNPs can be used as a novel antifungal agent in agriculture to control the plant pathogenic fungi of F. oxysporum and P. capsici.

Icephobic and Anticorrosion Coatings Deposited by Electrospinning on Aluminum Alloys for Aerospace Applications

“…In this case, the PVDF-HFP coating has low values of surface free energies due to the presence of fluoride functional groups [ 59 ]. A previous work has shown that this value is around 10 mN·m −1 [ 60 ], although this surface energy value strongly depends on the content of HFP in the PVDF-co-HFP because it is associated to an increase in the fluorine content in the polymer [ 61 ]. In Figure 12 , the FTIR spectrum of the PVDF-co-HFP electrospun fibers is shown.…”

Facile Synthesis of Carboxymethyl Cellulose Coated Core/Shell SiO2@Cu Nanoparticles and Their Antifungal Activity against Phytophthora capsici

“…Cu NPs can particularly be considered to be a promising fungicide against P. capsici. Pham et al [ 47 ] reported that the colloidal solution of Cu NPs showed superiority in growth inhibition over P. capsici. The particle size of Cu NPs significantly affected their antifungal activity.…”

Biogenic Nanoparticles: Synthesis, Characterisation and Applications

“…There are several experiments on using Fusarium oxysporum fungus to synthesise nanoparticles in several experiments, including many studies on AgNPs. Srivastava et al synthesised AgNPs (10-15 nm in size) using fungus Fusarium oxysporum [67], and demonstrated promising antibacterial effects on Escherichia coli and Pseudomonas aeruginosa. Other examples of nanoparticles produced using F. oxysporum include copper [68], gold [69] and magnesium oxide [70].…”