Preparation and characterization of beta-glucan particles containing a payload of nanoembedded rifabutin for enhanced targeted delivery to macrophages

Upadhyay, Tarun Kumar; Fatima, Nida; Sharma, Deepak; Saravanakumar, Veeramuthu; Sharma, Rolee

doi:10.17179/excli2016-804

Cited by 21 publications

(28 citation statements)

References 43 publications

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“…The peak at 2918 cm −1 was assigned to the symmetric and asymmetric C-H stretching vibrations of the methyl, methylene, and methoxy groups [47] . The peaks at 1733 and 1621 cm −1 were assigned to the C O stretching in the aldehyde group and C C stretching in aromatic compounds, respectively, while the peaks at 1219 and 1017 cm −1 were assigned to the stretching vibrations of the C-O stretching carboxylic acid group and C-N aromatic and aliphatic amines, respectively [48] , [49] , [50] , [51] . These bands shift in the spectra of SPE-AuNPs to 3267, 2917, 1720, 1618, 1197, and 1082 cm −1 .…”

Section: Resultsmentioning

confidence: 99%

Biogenic synthesis of gold nanoparticles mediated by Spondias dulcis (Anacardiaceae) peel extract and its cytotoxic activity in human breast cancer cell

et al. 2022

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Section: Resultsmentioning

confidence: 99%

Biogenic synthesis of gold nanoparticles mediated by Spondias dulcis (Anacardiaceae) peel extract and its cytotoxic activity in human breast cancer cell

et al. 2022

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“…RBITC was used to label YPs as previously reported. , Briefly, 100 mg of YPs was incubated in 10 mL of sodium carbonate buffer solution (0.1 M, pH 9.2) at 37 °C for 0.5 h, and then RBITC solution (1 mg/mL, dissolved in DMSO) was added dropwise and stirred overnight in the dark. The unreacted fluorescence probe was quenched in Tris buffer (1 M, pH 8.3) for 0.5 h. Then, the fluorescence-labeled YPs were well washed with sterile water, rinsed with ethanol and acetone to remove the water, and dried at room temperature in the dark.…”

Section: Methodsmentioning

confidence: 99%

Entrapping of Nanoparticles in Yeast Cell Wall Microparticles for Macrophage-Targeted Oral Delivery of Cabazitaxel

et al. 2018

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In this work, a nano-in-micro carrier was constructed by loading polymer-lipid hybrid nanoparticles (NPs) into porous and hollow yeast cell wall microparticles (YPs) for macrophage-targeted oral delivery of cabazitaxel (CTX). The YPs, primarily composed of natural β-1,3-d-glucan, can be recognized by the apical membrane receptor, dectin-1, which has a high expression on macrophages and intestinal M cells. By combining electrostatic force-driven self-deposition with solvent hydration/lyophilization methods, the positively charged NPs loaded with CTX or fluorescence probes were efficiently packaged into YPs, as verified by scanning electron microscope (SEM), atomic force mircoscope (AFM), and confocal laser scanning microscopy (CLSM) images. NP-loaded YPs (NYPs) showed a slower in vitro drug release and higher drug stability compared with NPs in a simulated gastrointestinal environment. Biodistribution experiments confirmed a widespread distribution and extended retention time of NYPs in the intestinal tract after oral administration. Importantly, a large amount of NYPs were primarily accumulated and transported in the intestinal Peyer's patches as visualized in distribution and absorption site studies, implying that NYPs were mainly absorbed through the lymphatic pathway. In vitro cell evaluation further demonstrated that NYPs were rapidly and efficiently taken up by macrophages via receptor dectin-1-mediated endocytosis using a mouse macrophage RAW 264.7 cell line. As expected, in the study of in vivo pharmacokinetics, the oral bioavailability of CTX was improved to 32.1% when loaded in NYPs, which is approximately 5.7 times higher than that of the CTX solution, indicating the NYPs are efficient for oral targeted delivery. Hence, this nano-in-micro carrier is believed to become a hopeful alternative strategy for increasing the oral absorption of small molecule drugs.

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“…Homogenization was carried out at 1,000 rpm for 10 min, and finally, the suspension was dried with the help of a Labultima spray dryer. The structure of yielded β-glucan was elaborated with Fourier transform IR (FTIR) analysis, and an anthrone test was carried out for the confirmation of the nature of the carbohydrate ( 22 ).…”

Section: Methodsmentioning

confidence: 99%

In vitro elucidation of antioxidant, antiproliferative, and apoptotic potential of yeast-derived β-1,3-glucan particles against cervical cancer cells

et al. 2022

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Cancer is the leading cause of mortality worldwide and in particular is the fourth most common cause of mortality in women every year. Conventional treatments for cancer are chemotherapy and radiation therapy, which have various kinds of side effects. Hence, there is a high need to develop alternative, efficient, and safer therapies for cancer treatment. β-Glucan, a novel polysaccharide isolated from baker’s yeast Saccharomyces cerevisiae, shows noteworthy cytotoxicity toward a variety of cancer cell lines in vitro. In this research, we characterized the β-glucan with high-performance thin-layer chromatography (HPTLC) analysis and found that d-glucose units with β-1,3 links are the major component of the extracted β-glucan particles. Fourier transform IR (FTIR) analysis confirmed a β-(1→3)-linked glucan structure. In vitro cell cytotoxicity was evaluated by MTT with IC50 136 μg/ml, and therapeutic potential was assessed by various assays using values below and above the IC50. A significant reactive oxygen species (ROS) generation at 50–150 μg/ml of concentrations indicated the apoptosis of cervical cancer cells. Along with ROS generation, these concentrations were also found to induce morphological changes such as fragmentation in DNA upon staining HeLa cells with DAPI. Mitochondrial membrane potential was significantly reduced after increasing the dose of treatment, assessed with the help of MitoTracker dye. Hence, by all these experimental supports, we observed that β-glucan has the potential to slow down the growth of cervical cancer cells, and it can be further investigated for unfolding its complete anticancer potential.

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Preparation and characterization of beta-glucan particles containing a payload of nanoembedded rifabutin for enhanced targeted delivery to macrophages

Cited by 21 publications

References 43 publications

Biogenic synthesis of gold nanoparticles mediated by Spondias dulcis (Anacardiaceae) peel extract and its cytotoxic activity in human breast cancer cell

Biogenic synthesis of gold nanoparticles mediated by Spondias dulcis (Anacardiaceae) peel extract and its cytotoxic activity in human breast cancer cell

Entrapping of Nanoparticles in Yeast Cell Wall Microparticles for Macrophage-Targeted Oral Delivery of Cabazitaxel

In vitro elucidation of antioxidant, antiproliferative, and apoptotic potential of yeast-derived β-1,3-glucan particles against cervical cancer cells

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