Preparation and characterization of calcium oxalate dihydrate seeds suitable for crystal growth kinetic analyses

Stanković, Anamarija; Kontrec, Jasminka; Džakula, Branka Njegić; Kovačević, Davor; Marković, Branimir; Kralj, Damir

doi:10.1016/j.jcrysgro.2018.08.020

Cited by 9 publications

(10 citation statements)

References 61 publications

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“…Figure 4(b) and Table 3 show the FT-IR spectra of CaOx crystal as CSP0 concentration increased, υ as (COO-) blue shift continuously. In the fingerprint region, the absorption bands of COD crystals were at 912 and 668 cm -1 , which were obviously different from 951, 887, and 518 cm -1 of COM, of which 887 and 783 cm -1 belonged to the COM C-C stretching vibration and O-C-O in-plane bending vibration, respectively [38].…”

Section: Effect Of Csp0 Concentration On Calcium Oxalate Crystalmentioning

confidence: 91%

“…This observation showed that the percentage of COM gradually decreased while COD gradually increased. Given that ν as (COO-) of COM and COD occur at 1,618 and 1,653 cm -1 , respectively, ν s (COO-) of COM and COD were 1,316 and 1,324 cm -1 , respectively (Figure 3(d)) [37,38]. The blue shift values of ν as (COO-) and ν s (COO-) depended on the percentage of COD crystals in the COM-COD mixture.…”

Section: Antioxidant Capacity Of Corn Silk Polysaccharides With Diffementioning

confidence: 99%

“…The zeta potential is a measurement of electrostatic repulsive force between particles. When the crystal surface has a high charge density, the absolute value of zeta potential is larger, the crystals do not aggregate easily, and the stability in the solution increases [38]. Figure 6 shows the zeta potentials of the formed CaOx crystal induced by CSPs.…”

Section: Effects Of Corn Silk Polysaccharides On Zeta Potential Ofmentioning

confidence: 99%

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Modulation of Calcium Oxalate Crystal Growth and Protection from Oxidatively Damaged Renal Epithelial Cells of Corn Silk Polysaccharides with Different Molecular Weights

Chen

Sun

Ouyang

2020

Oxidative Medicine and Cellular Longevity

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Corn silk polysaccharide (CSP0; molecular weight=124 kDa) was degraded by ultrasonication to obtain five degraded polysaccharides, namely, CSP1, CSP2, CSP3, CSP4, and CSP5, with molecular weights of 26.1, 12.2, 6.0, 3.5, and 2.0 kDa, respectively. The structures of these polysaccharides were characterized by FT-IR, 1H NMR, and 13C NMR analyses. The antioxidant activities, including scavenging ability for hydroxyl radicals and DPPH free radicals, chelation ability for Fe2+ ions, and reducing ability of CSP increased with decreased molecular weight of CSPs within 6.0 to 124 kDa. However, antioxidant activity weakened when the molecular weight of CSPs reached 3.5 and 2 kDa. CSP3 with a molecular weight of 6.0 kDa exhibited the strongest antioxidant activity. After protection with 60 μg/mL CSPs, the viability of human renal proximal tubular epithelial cells (HK-2) damaged by nano-COM crystals increased, the level of reactive oxygen species decreased, and the amount of COM crystal adhered onto the cell surface decreased. The ability of CSPs to protect cells from CaOx crystal damage was consistent with their antioxidant activity. CSPs can specifically combine with CaOx crystal to inhibit the conversion of calcium oxalate dihydrate crystal to calcium oxalate monohydrate crystal. All these results showed that the activity of CSPs was closely correlated with molecular weight. A very high or low molecular weight of CSPs was not conducive to their activity. CSPs, especially CSP3 with a molecular weight of 6.0 kDa, can be used as a potential antistone drug.

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Section: Effect Of Csp0 Concentration On Calcium Oxalate Crystalmentioning

confidence: 91%

Section: Antioxidant Capacity Of Corn Silk Polysaccharides With Diffementioning

confidence: 99%

Section: Effects Of Corn Silk Polysaccharides On Zeta Potential Ofmentioning

confidence: 99%

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Modulation of Calcium Oxalate Crystal Growth and Protection from Oxidatively Damaged Renal Epithelial Cells of Corn Silk Polysaccharides with Different Molecular Weights

Chen

Sun

Ouyang

2020

Oxidative Medicine and Cellular Longevity

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“…Effect of PCP-Cs on the Zeta Potential of Crystals. The particle surface with a high charge density has a large absolute value of the Zeta potential and electrostatic repulsion force between particles, it cannot easily aggregate, and it is stable in solution [43]. The Zeta potentials of CaOx crystals generated by different PCP-Cs are shown in Figure 9(a).…”

Section: Regulation Of Pcp-cs On Caoxmentioning

confidence: 99%

Inhibition of Calcium Oxalate Formation and Antioxidant Activity of Carboxymethylated Poria cocos Polysaccharides

Liu

Zhao

et al. 2021

Oxidative Medicine and Cellular Longevity

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Three carboxymethylated Poria cocos polysaccharides (PCP-C1, PCP-C2, and PCP-C3) with -COOH contents of 6.13%, 10.24%, and 16.22%, respectively, were obtained by carboxymethylation of the original polysaccharide (PCP-C0), which has a molecular weight of 4 kDa and a carboxyl (-COOH) content of 2.54%. The structure of the PCP-Cs was characterized by FT-IR, 1H NMR, and 13C NMR spectra. The four PCP-Cs exhibited antioxidant activity, and their ability to scavenge radicals (hydroxyl and DPPH) and chelate ferrous ions was positively correlated with the degree of carboxymethylation. As the content of -COOH groups in the PCP-Cs increases, their ability to regulate the growth of calcium oxalate (CaOx) crystals was enhanced, thus inhibiting the growth of calcium oxalate monohydrate (COM) crystals and inducing the formation of more calcium oxalate dihydrate (COD) crystals. The formed CaOx crystal was more round and blunt, the absolute value of the Zeta potential on the crystal surface increased, and the aggregation between crystals was inhibited. Thermogravimetric analysis curves showed that the proportions of PCP-C0, PCP-C1, PCP-C2, and PCP-C3 incorporated into the crystal were 20.52%, 15.60%, 10.65%, and 9.78%, respectively, in the presence of 0.4 g/L PCP-Cs. PCP-C protection resisted oxidative damages of human kidney proximal tubular epithelial cells (HK-2) caused by oxalate, resulting in increased cell viability and superoxide dismutase activity and decreased reactive oxygen species levels, malondialdehyde content, and 8-hydroxy-deoxyguanosine expression. Hence, PCP-Cs, especially PCP-C3, can inhibit the formation of CaOx crystals and may have the potential to be an alternative antistone drug.

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“…The obtained results suggest that changes in morphology could be related to the presence of different ions in system and difference in system supersaturation. At that, crystal surface charge should be considered as a relevant parameter that could control crystal morphology . The literature data show that surface charge of the COM crystals is related to the structure of crystallographic {100} and {010} faces .…”

Section: Resultsmentioning

confidence: 99%

Calcium Oxalate Precipitation in Model Systems Mimicking the Conditions of Hyperoxaluria

Stanković

Šafranko

Kontrec

et al. 2019

Crystal Research and Technology

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This work focuses on systematic research of spontaneous precipitation of calcium oxalate in model systems mimicking the chemical conditions typical for hyperoxaluria. For this purpose, model systems of three different complexities (simple, NaCl, and artificial urine system) and two initial pH (pH i = 5.0 and pH i = 9.0) at 37°C are studied. In all investigated systems, dominant precipitation of calcium oxalate monohydrate (COM) is observed, except in artificial urine system at pH i = 9.0, c i (C 2 O 4 2− ) ࣙ 6.0 mmol dm −3 and c i (Ca 2+ ) ࣙ 7.5 mmol dm −3 , where precipitation of a mixture of calcium oxalate monohydrate and calcium oxalate dihydrate occurs. The significant difference is mainly observed in COM crystal morphology. In a simple system, COM precipitates in a dendritic form; in NaCl system, dendritic form and irregular shapes are found, while in the artificial urine, prismatic hexagonal and aggregated COM crystals precipitate.

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Preparation and characterization of calcium oxalate dihydrate seeds suitable for crystal growth kinetic analyses

Cited by 9 publications

References 61 publications

Modulation of Calcium Oxalate Crystal Growth and Protection from Oxidatively Damaged Renal Epithelial Cells of Corn Silk Polysaccharides with Different Molecular Weights

Modulation of Calcium Oxalate Crystal Growth and Protection from Oxidatively Damaged Renal Epithelial Cells of Corn Silk Polysaccharides with Different Molecular Weights

Inhibition of Calcium Oxalate Formation and Antioxidant Activity of Carboxymethylated Poria cocos Polysaccharides

Calcium Oxalate Precipitation in Model Systems Mimicking the Conditions of Hyperoxaluria

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