Preparation and Characterization of Clinoenstatite Bioceramics

Abstract

Clinoenstatite (MgSiO 3 ) powders were synthesized by precipitation process using magnesium nitrate (Mg(NO 3 ) 2 . 6H 2 O) and sodium silicate (Na 2 SiO 3 . 9H 2 O), and clinoenstatite ceramics were prepared by sintering its powder compacts at 14001C for 3 and 5 h. The shrinkage (4.53%), bending strength (31.78 MPa), relative density (82.84%), and Young's modulus (8.51 GPa) were investigated. The percentages of weight loss of the clinoenstatite ceramics were listed as 1.2970.55%, 1.3670.30%, 7.3171.54%, and 10.7770.93% after 1-, 3-, 7-, and 14-day soaking in the Tris-HCl solution. The biocompatibility studies showed that clinoenstatite ceramics could not induce the formation of HAp in simulated body fluid, but the ionic products of the clinoenstatite powder extracts promoted the proliferation of the mouse fibroblasts (L929 cell). The murine embryonic mesenchymal stem cells adhered well and proliferated more on the clinoenstatite ceramics than on the traditional hydroxyapatite ceramics. These results showed that clinoenstatite bioceramics possessed good cell biocompatibility and might be used for preparation of bone implants or biocoatings.

Clinoenstatite coatings have high bonding strength, bioactive ion release, and osteoimmunomodulatory effects that enhance in vivo osseointegration

“…Clinoenstatite (CLT, MgSiO 3 ) is a bioceramic that contains both Mg and Si and combines mechanical strength with chemical stability and cytocompatability, making it an ideal coating material for our purposes [22,23].…”

“…Clinoenstatite (CLT, MgSiO 3 ) powders were synthesized by the sol-gel method using tetraethyl orthosilicate ((C 2 H 5 O) 4 Si, TEOS) (with purity≥97.1%) and magnesium nitrate hexahydrate (Mg(NO 3 ) 2 ·6H 2 O) (with purity≥99.0%) as raw materials [35]. A sinter-crushing method was applied to prepare CLT particles with diameter of 40-80 µm (as shown in Supplementary Figure 2) for the following coating preparation.…”

Clinoenstatite/Tantalum Coating for Enhancement of Biocompatibility and Corrosion Protection of Mg Alloy

“…Subsequently, EPD as a highly flexible, effortless and the most affordable approach was performed to fabricate clinoenstatite (CLT; MgSiO 3 ) as the top-layer to enhance formation of apatite and biocompatibility of the Mg alloy substrate [20,21]. CLT is part of Mg containing silicate-based ceramic groups and is applied for artificial bone and dental root because of its outstanding formation of calcium-phosphate, biocompatibility and better mechanical characteristics such as adhesive strength and fracture toughness in comparison with hydroxyapatite [22,23].…”

A comparative study of proliferation and osteogenic differentiation of rat adipose-derived stem cells in β-tricalcium phosphate (β-TCP), forsterite (Mg2SiO4) and clinoenstatite (MgSiO3)

“…Our previous work reported another new magnesium-silicate ceramic, clinoenstatite (MgSiO 3 , MS), and revealed that the ionic products of the MS powder extracts can promote the proliferation of mouse fibroblasts (L929 cell); and the murine embryonic mesenchymal stem cells adhered well; higher proliferation capacities on the clinoenstatite ceramics were also observed than that on the traditional hydroxyapatite ceramics [12]. These studies suggested that forsterite and clinoenstatite may be the potential and attractive bioactive ceramics for BTE.…”

“…β-TCP, M 2 S and MS ceramic discs with dimensions of 8 mm×0.8 mm were prepared as described previously [8,11,12]. The ceramic discs were sonicated for 5 min, rinsed with distilled water and radiosterilized.…”