Preparation and characterization of control materials for the analysis of conjugated linoleic acid and trans-vaccenic acid in beef

Phillips, Katherine M.; Ruggio, David M.; Howe, Juliette C.; Leheska, J. M.; Smith, Stephen B.; Engle, T. E.; Rasor, Amy S.; Conley, Nancy A.

doi:10.1016/j.foodres.2010.06.012

Cited by 16 publications

(7 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reported concentrations of cis-9, trans-11 CLA may also contain trace amounts of trans-7, cis-9 CLA. The 18:1 trans-isomers were identified as described previously (Phillips et al, 2010). Individual FAME was quantified as a percentage of total FAME analyzed.…”

Section: Fatty Acidsmentioning

confidence: 99%

Consumer sensory evaluation, fatty acid composition, and shelf-life of ground beef with subcutaneous fat trimmings from different carcass locations

et al. 2015

Self Cite

View full text Add to dashboard Cite

Section: Fatty Acidsmentioning

confidence: 99%

Consumer sensory evaluation, fatty acid composition, and shelf-life of ground beef with subcutaneous fat trimmings from different carcass locations

et al. 2015

Self Cite

View full text Add to dashboard Cite

“…Fatty acids.-Plasma samples were analyzed for fatty acids as described by Phillips et al (2010). Samples were methylated as described in Parks and Goins (1994) and were reconstituted in hexane prior to analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Methyl esters were injected into a gas chromatograph (6890 Series II; Hewlett Packard, Avondale, Pennsylvania) fitted with a flame ionization detector and an automatic injector. Individual fatty acids were identified by comparing their retention times to those of known standards (12:0 and 27:0) that were added into each sample prior to methylation (Phillips et al 2010). Fatty acid results are expressed as weight percentages of total fatty acids.…”

Section: Methodsmentioning

confidence: 99%

Baseline Health and Nutritional Parameters of Wild Sand Tigers Sampled in Delaware Bay

Hoopes

Clauss²,

Wetherbee

et al. 2022

J Aqua Anim Hlth

View full text Add to dashboard Cite

Species-specific hematological reference values are essential for diagnosis and treatment of disease and maintaining overall health of animals. This information is lacking for many species of elasmobranchs maintained in zoos and aquaria, thus reducing the effectiveness of care for these animals. Descriptive statistics and reference intervals were calculated for hematocrit and complete blood cell counts, biochemistry and protein electrophoresis parameters, trace minerals, vitamins, heavy metals, reproductive hormones, and fatty acids in the blood of 153 wild Sand Tigers Carcharias taurus of both sexes and a range of sizes caught in Delaware Bay (Delaware, USA). Mean hematocrit, total white blood cell counts, lymphocyte differentials, glucose, phosphorus, amylase, and aspartate aminotransferase levels were significantly higher in juveniles than in adults. Levels of estradiol, progesterone, testosterone, and differences in selenium and eicosapentaenoic acid (a polyunsaturated fatty acid) between males and females suggest that they are important parameters for improving Sand Tiger breeding success in managed care. Finally, blood metal levels for arsenic, cadmium, lead, and mercury suggest low levels of contaminant exposure for Sand Tigers during their summer residence in Delaware Bay. The results of this study provide baseline health parameters for wild Sand Tigers that will aid in effective maintenance of aquarium animals and contribute to a greater understanding of the biology of these sharks and efforts to accomplish sustainable management of their populations.Species-specific hematological parameters are important tools for evaluating and understanding health and nutrition in both wild and managed populations of animals.Diet, environmental features, activity level, social structure, stress, and parasite loads are all factors that may influence blood values in zoo and aquarium animal

show abstract

“…Since a different acid catalyst for FAMEs production was used by Bicalho and co-workers [32], in this work methylation times of 30 and 60 min were tested. Additionally, considering that the inclusion of saponification reaction is frequently mentioned in FA analysis of other matrices, such as vegetable oils [39,40] and meat [41], the inclusion of saponification was also assayed, resulting in a total of four one-step protocols tested. For all methodologies, based on literature data, BHT was used as antioxidant to prevent samples FA degradation, C19:0 was used as IS and BF 3 as a catalyst for FA methylation.…”

Section: Comparison Of Sample Preparation Methodologiesmentioning

confidence: 99%

Simple Methodology for the Quantitative Analysis of Fatty Acids in Human Red Blood Cells

et al. 2015

View full text Add to dashboard Cite

methods, in which lipid extraction and derivatization were performed simultaneously. For the one-step methods, different methylation periods and the inclusion of a saponification reaction were evaluated. Differences in absolute FA concentrations were observed among the tested methods, in particular for some metabolically relevant FAs such as trans elaidic acid and eicosapentaenoic acid. The one-step method with saponification and 60 min of methylation time was selected since it allowed the identification of a higher number of FAs, and was further submitted to in-house validation. The proposed methodology provides a simple, fast and accurate tool to quantitatively analyse FAs in human RBCs, useful for clinical and nutritional studies.

show abstract

Preparation and characterization of control materials for the analysis of conjugated linoleic acid and trans-vaccenic acid in beef

Cited by 16 publications

References 41 publications

Consumer sensory evaluation, fatty acid composition, and shelf-life of ground beef with subcutaneous fat trimmings from different carcass locations

Consumer sensory evaluation, fatty acid composition, and shelf-life of ground beef with subcutaneous fat trimmings from different carcass locations

Baseline Health and Nutritional Parameters of Wild Sand Tigers Sampled in Delaware Bay

Simple Methodology for the Quantitative Analysis of Fatty Acids in Human Red Blood Cells

Contact Info

Product

Resources

About