Preparation and characterization of dispersible chitosan particles with borate crosslinking and their antimicrobial and antifungal activity

Saita, Kanako; Nagaoka, Shoji; Shirosaki, Tomohiro; Horikawa, Maki; Matsuda, Shigeki; Ihara, Hirotaka

doi:10.1016/j.carres.2011.12.017

Cited by 51 publications

(25 citation statements)

References 27 publications

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“…The XRD pattern of chitosan in this study displayed two sharp diffraction peaks at 2 θ = 10° and 20°, revealing the high crystallinity of chitosan. This result is consistent with the result of a number of previous studies [22,32,33], which reported the typical X-ray diffraction pattern of chitosan. The high crystallinity is due to the chitosan structure while plenty of hydroxyl and amino groups could form strong intermolecular and intramolecular hydrogen bonds [33].…”

Section: Resultssupporting

confidence: 93%

“…The non-inhibitory effect of chitosan may be due to the fact that chitosan is incapable to diffuse through the adjacent agar media when it is in a solid form [32]. Furthermore, this result is consistent with a number of previous studies that demonstrated chitosan particles would exhibit their potential in suppressing the bacterial growth only when they are in acidic media, in which the NH 2 group in chitosan becomes a quaternary amino group and allows the chitosan to inhibit the growth of a variety of bacteria [11,33,34]. …”

Section: Resultssupporting

confidence: 87%

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Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde

et al. 2013

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This present study deals with synthesis, characterization and antibacterial activity of cross-linked chitosan-glutaraldehyde. Results from this study indicated that cross-linked chitosan-glutaraldehyde markedly inhibited the growth of antibiotic-resistant Burkholderia cepacia complex regardless of bacterial species and incubation time while bacterial growth was unaffected by solid chitosan. Furthermore, high temperature treated cross-linked chitosan-glutaraldehyde showed strong antibacterial activity against the selected strain 0901 although the inhibitory effects varied with different temperatures. In addition, physical-chemical and structural characterization revealed that the cross-linking of chitosan with glutaraldehyde resulted in a rougher surface morphology, a characteristic Fourier transform infrared (FTIR) band at 1559 cm−1, a specific X-ray diffraction peak centered at 2θ = 15°, a lower contents of carbon, hydrogen and nitrogen, and a higher stability of glucose units compared to chitosan based on scanning electron microscopic observation, FTIR spectra, X-ray diffraction pattern, as well as elemental and thermo gravimetric analysis. Overall, this study indicated that cross-linked chitosan-glutaraldehyde is promising to be developed as a new antibacterial drug.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 87%

Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde

et al. 2013

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“…Three prominent crystalline peaks were observed in both specimens indicating nanospheres stability. This may be attributed to the special characters of chitosan polymer, namely the presence of plenty of hydroxyl and amino groups forming strong intra/inter molecular hydrogen bonds (Saita et al 2012).…”

Section: Discussionmentioning

confidence: 99%

The effect of chitosan nanospheres on the immunogenicity of Toxoplasma lysate vaccine in mice

et al. 2014

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Toxoplasmosis, a zoonotic parasitic disease, is a huge challenge for which there is no effective vaccine up till now. In this study, chitosan nanospheres encapsulated with Toxoplasma lysate vaccine was evaluated for its ability to protect mice against both acute and chronic toxoplasmosis models of infection. Results showed that chitosan nanospheres were equally effective to Freund's incomplete adjuvant (FIA) in enhancing the efficacy of Toxoplasma lysate vaccine. The effectiveness was demonstrated by the delayed death of vaccinated mice following challenge either with virulent RH or avirulent Me49 strains, the significant decrease in parasite density in different organs, significant increase in the humoral and cellular immune response (IgG and IFN c) with a marked reduction of pathological changes in the different organs. However chitosan nanospheres were superior to FIA due to their cost effective preparation and much less necrotic changes induced in the studied organs. The success of chitosan polymer as an alternative to commonly used adjuvants paves the way for the use of other newly developed polymers to be used in the field of vaccine development.

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“…Nevertheless, such hybrid assembly is expected to benefit from material design [14,[81][82][83], immobilization on solid substrates [84] and cross-linking [85,86]. The specimens were thus lyophilized to obtain composite films, which were analyzed at the solid state by ESEM.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Chitosan-Polyoxometalate Nanocomposites: Synthesis, Characterization and Application as Antimicrobial Agents

et al. 2014

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Polyoxometalates (POMs) were used, together with chitosan (CS), to obtain hybrid nanoaggregates. Three representative POMs were efficiently assembled into nanoparticles of few hundred nm diameter, featuring entangled ribbons substructure. In order to establish suitable preparation and stability conditions, the assemblies were characterized in solution by UV-Vis spectroscopy, dynamic light scattering and f-potential. The nanoparticles were tested against E. coli (10 6 CFU/ ml) in aqueous solution, showing a synergic activity of the heteropolyacid H 5 PMo 10 V 2 O 40 and CS. For such components, a highly porous and antibacterial film was obtained upon lyophilisation of the colloidal mixture.

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Preparation and characterization of dispersible chitosan particles with borate crosslinking and their antimicrobial and antifungal activity

Cited by 51 publications

References 27 publications

Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde

Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde

The effect of chitosan nanospheres on the immunogenicity of Toxoplasma lysate vaccine in mice

Chitosan-Polyoxometalate Nanocomposites: Synthesis, Characterization and Application as Antimicrobial Agents

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