Preparation and characterization of egg yolk immunoglobulin Y specific to influenza B virus

Wen, Junlin; Zhao, Suqing; He, Daigui; Yang, Yuane; Li, Yueming; Zhu, Shimin

doi:10.1016/j.antiviral.2011.11.005

Cited by 65 publications

(54 citation statements)

References 19 publications

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“…Using the prepared whole-cell antigen, a female New Zealand white rabbit weighing 2 kg was immunized intramuscularly for a total of four times at intervals of ten days for producing polyclonal antibody against S. oneidensis . The obtained antisera were purified with ammonium sulfate precipitation and characterized by enzyme-linked immunosorbent assay according to a previous protocol36. The purified anti- S. oneidensis antibodies were stored at −20°C.…”

Section: Methodsmentioning

confidence: 99%

Colorimetric detection of Shewanella oneidensis based on immunomagnetic capture and bacterial intrinsic peroxidase activity

Wen

Zhou

Chen

2014

Sci Rep

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Rapid detection and enumeration of target microorganisms is considered as a powerful tool for monitoring bioremediation process that typically involves cleaning up polluted environments with functional microbes. A novel colorimetric assay is presented based on immunomagnetic capture and bacterial intrinsic peroxidase activity for rapidly detecting Shewanella oneidensis, an important model organism for environmental bioremediation because of its remarkably diverse respiratory abilities. Analyte bacteria captured on the immunomagnetic beads provided a bacterial out-membrane peroxidase-amplified colorimetric readout of the immunorecognition event by oxidizing 3, 3′, 5, 5′-tetramethylbenzidine (TMB) in the present of hydrogen peroxide. The high-efficiency of immunomagnetic capture and signal amplification of peroxidase activity offers an excellent detection performance with a wide dynamic range between 5.0 × 103 and 5.0 × 106 CFU/mL toward target cells. Furthermore, this method was demonstrated to be feasible in detecting S. oneidensis cells spiked in environmental samples. The proposed colorimetric assay shows promising environmental applications for rapid detection of target microorganisms.

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Section: Methodsmentioning

confidence: 99%

Colorimetric detection of Shewanella oneidensis based on immunomagnetic capture and bacterial intrinsic peroxidase activity

Wen

Zhou

Chen

2014

Sci Rep

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“…Escherichia coli, Salmonella, Staphylococcus, Coronavirus, Rotavirus (Kovacs-Nolan, et al, 2004;Wen, et al, 2012;Xu, et al, 2011;Liou, et al, 2011). Recently it has been shown that hen's egg IgY preparations stimulate the whole blood cells to release TNF-α and IL-1β cytokines.…”

Section: Lipidomics Analysis Of Egg Yolk and Application In Medicinementioning

confidence: 96%

Hyphenated Analytical Methods in Determination of Biologically Active Compounds in Hen's Eggs

Walczak

Bocian

Trziszka

et al. 2015

Critical Reviews in Analytical Chemistry

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Hen's egg is a complete material needed for the development of the embryo; it is an important source of nutraceutical compounds, such as protein, fats, vitamins, trace metals, and minerals. Moreover, avian egg contains biologically active compounds that exhibit antibacterial and antimicrobial activities as well as antitumor, antiviral, antioxidant, immunomodulating, and therapeutic properties. Eggs are mostly very good sources of valuable, easily digestible proteins. This review focuses on the biologically active compounds from hen's egg and applications of these compounds in medicine and the pharmaceutical industry. Additionally, it gives an overview of the hyphenated separation techniques, including sample preparation, analysis, and identification, used in the proteomics and lipidomics analysis.

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“…In recent years, specific IgY has received considerable attention in passive immunotherapy for infectious disease due to a number of advantages (Dai et al, 2013;Wen et al, 2012). IgY can be extracted in high yield and recovered from immunized hens over prolonged periods.…”

Section: Discussionmentioning

confidence: 99%

“…The use of IgY may help lower costs of immunological testing. IgY has been used in several studies for diagnostics and therapy (Dai et al, 2013;Wen et al, 2012).…”

Section: Virusesmentioning

confidence: 99%

Expression of Lily mottle virus Coat Protein and Preparation of IgY Antibody against the Recombinant Coat Protein

Yoo¹,

Jung²

2014

HST

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Lily symptomless virus (LSV), Lily mottle virus (LMoV), and Cucumber mosaic virus (CMV) are the most prevalent viruses infecting lilies in Korea. Leaf and bulb samples showing characteristic symptoms of virus infection were collected in 2012, and 80 field samples were analyzed by reverse transcription polymerase chain reaction (RT-PCR). The infection frequencies were 79% for LMoV, 5% for LSV, and 3% for CMV. The LMoV coat protein gene was amplified and cloned into the pET21d(+) expression vector to develop serological diagnostic tools to detect LMoV. The resulting carboxy-terminal His-tagged coat proteins were expressed in Escherichia coli strain BL21 (DE3) by induction with IPTG. The recombinant proteins were purified using Ni-NTA agarose beads and used as an antigen to produce polyclonal antibodies in laying hens. The resulting egg yolk immunoglobulin (IgY) specifically recognized LMoV from infected plant tissues in immunoblotting assays and had comparable sensitivity to that of a mammalian antibody. In addition, method of immunocapture RT-PCR using this IgY was developed for sensitive, efficient, and rapid detection of LMoV. Based on these results, large-scale bulb tests and detection of LMoV in epidemiological studies can be performed routinely using this IgY. This is the first report of production of a polyclonal IgY against a plant virus and its use for diagnosis. Additional key words: egg yolk immunoglobulin, IC-RT-PCR

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Preparation and characterization of egg yolk immunoglobulin Y specific to influenza B virus

Cited by 65 publications

References 19 publications

Colorimetric detection of Shewanella oneidensis based on immunomagnetic capture and bacterial intrinsic peroxidase activity

Colorimetric detection of Shewanella oneidensis based on immunomagnetic capture and bacterial intrinsic peroxidase activity

Hyphenated Analytical Methods in Determination of Biologically Active Compounds in Hen's Eggs

Expression of Lily mottle virus Coat Protein and Preparation of IgY Antibody against the Recombinant Coat Protein

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