Preparation and characterization of electrospun curcumin loaded poly(2-hydroxyethyl methacrylate) nanofiber-A biomaterial for multidrug resistant organisms

Ramalingam, Nithya; Natarajan, T. S.; Rajiv, Sheeja

doi:10.1002/jbm.a.35138

Cited by 48 publications

(22 citation statements)

References 24 publications

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“…Relatively high amounts of curcumin have been incorporated in electrospun materials, as most of the studies carried out propose proportions of curcumin 1% of the polymer weight. [19][20][21][22]25,28 These materials often lead to concentrations near or above the apoptosis threshold of 10 μM. Although several authors claim benefits from such materials to stimulate repair, it is not clear why these amounts have been selected while data from studies carried out with free curcumin suggest that lower concentrations might be more adequate for wound healing applications.…”

Section: Introductionmentioning

confidence: 99%

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Mouthuy¹,

Škoc²,

Gašparović³

et al. 2017

IJN

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Electrospun filaments represent a new generation of medical textiles with promising applications in soft tissue repair. A potential strategy to improve their design is to combine them with bioactive molecules. Curcumin, a natural compound found in turmeric, is particularly attractive for its antioxidant, anti-inflammatory, and antimicrobial properties. However, investigating the range of relevant doses of curcumin in materials designed for tissue regeneration has remained limited. In this paper, a wide range of curcumin concentrations was explored and the potential of the resulting materials for soft tissue repair applications was assessed. Polydioxanone (PDO) filaments were prepared with various amounts of curcumin: 0%, 0.001%, 0.01%, 0.1%, 1%, and 10% (weight to weight ratio). The results from the present study showed that, at low doses (≤0.1%), the addition of curcumin has no influence on the spinning process or on the physicochemical properties of the filaments, whereas higher doses lead to smaller fiber diameters and improved mechanical properties. Moreover, filaments with 0.001% and 0.01% curcumin stimulate the metabolic activity and proliferation of normal human dermal fibroblasts (NHDFs) compared with the no-filament control. However, this stimulation is not significant when compared to the control filaments (0%). Highly dosed filaments induce either the inhibition of proliferation (with 1%) or cell apoptosis (with 10%) as a result of the concentrations of curcumin found in the medium (9 and 32 μM, respectively), which are near or above the known toxicity threshold of curcumin (~10 μM). Moreover, filaments with 10% curcumin increase the catalase activity and glutathione content in NHDFs, indicating an increased production of reactive oxygen species resulting from the large concentration of curcumin. Overall, this study suggested that PDO electrospun filaments loaded with low amounts of curcumin are more promising compared with higher concentrations for stimulating tissue repair. This study also highlighted the need to explore lower concentrations when using polymers as PDO, such as those with polycaprolactone and other degradable polyesters.

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Section: Introductionmentioning

confidence: 99%

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Mouthuy¹,

Škoc²,

Gašparović³

et al. 2017

IJN

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“…The formed formazan crystals were then dissolved by adding 100 µL of DMSO and this was mixed properly by pipetting up and down. Spectrophotometrical absorbance of the purple blue formazan dye was measured in a microplate reader at 570 nm (Biorad 680) [20,29]. Cytotoxicity was determined using Graph pad prism 6 software.…”

Section: Isolation Of Hap From Chicken Bonementioning

confidence: 99%

Development of Porous Polysaccharides-Hydroxyapatite Composite Scaffolds via Freeze Drying Method and in vitro Osteoconductivity on MG 63 Cells

Rajesh¹,

Kumar²,

Kenie³

et al. 2018

Asian J. Chem.

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“…Ramalingam et al used electrospinning method to produce poly (2-hydroxyethyl methacrylate) p(HEMA) nanofibers containing different concentrations of curcumin. They reported that these nanofibers had high antibacterial activity against E. coli and S. aureus bacteria [7]. In another study, functionalized PCL/PVP nanofibers were produced by the electrospinning method by combining Tecomell undulata plant extracts which have therapeutic property for wound healing against typical hospital pathogens of P. aeruginosa, S. aureus, and E. coli [8].…”

Section: Introductionmentioning

confidence: 99%

Functional Composite Nanofibers Derived From Natural Extract of Satureja Hortensis

Gergeroglu¹,

Avcı²

2017

Anadolu University Journal of Science and Technology-a Applied Sciences and Engineering

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Development of environmentally friendly and natural-based products with healing properties has become a popular trend for human health. Here, for the first time as far as known, we reported an efficient antibacterial composite nanofiber which was functionalized with the herbal extract of Satureja hortensis (SH) via electrospinning technique for wound care dressing applications. The plant which was used for this purpose is cultivated on a commercial scale in our country, Turkey, and it is relatively inexpensive and widely used. Investigation of the herbal extract demonstrated 227% higher antibacterial activity against Staphylococcus aureus and 141% higher for Pseudomonas aeruginosa when compared with a commonly used strong antibiotic of amoxicillin. Thermoplastic polyurethane (TPU)-based composite nanofibers including SH with a diameter of about 734 nm for the wound dressings also showed efficient antibacterial activity against these two pathogens which can lead significant health problems and deaths. FTIR characterization confirmed that there is no chemical change of SH when it was incorporated to the composite polymeric nanofibers by the electrospinning technique. We strongly believe that the obtained results suggest a potential natural-based eco-friendly approach for wound care dressing in contrast with synthetic and metal based-antibacterial agents, which have many negative effects on human body and the environment.

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Preparation and characterization of electrospun curcumin loaded poly(2-hydroxyethyl methacrylate) nanofiber-A biomaterial for multidrug resistant organisms

Cited by 48 publications

References 24 publications

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Development of Porous Polysaccharides-Hydroxyapatite Composite Scaffolds via Freeze Drying Method and in vitro Osteoconductivity on MG 63 Cells

Functional Composite Nanofibers Derived From Natural Extract of Satureja Hortensis

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