Preparation and characterization of electrospun graphene/silk fibroin conductive fibrous scaffolds

Yang, Yi; Ding, Xia; Zou, Tongqiang; Ge, Peng; Liu, Haifeng; Fan, Yubo

doi:10.1039/c6ra26807b

Cited by 41 publications

(49 citation statements)

References 43 publications

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“…SF is a natural polymer which attracts a lot of attention for biological applications specifically for tissue engineering due to its good mechanical and cytocompatibility properties. SF nanofibers fabricated by electrospinning have some advantages like higher surface to area than other fabrication methods, which can mimic natural ECM in several ways …”

Section: Discussionmentioning

confidence: 99%

“…SF nanofibers fabricated by electrospinning have some advantages like higher surface to area than other fabrication methods, which can mimic natural ECM in several ways. 49 Graphene and reduced graphene oxide (rGO) have been used for many biomedical applications such as biosensing, cancer targeting, electrical stimulation of cells, and tissue because of their outstanding properties such as outstanding electrical conductivity. 50,51 In our study, SF-rGo fibrous scaffold fabricated using electrospinning and the resistance of the silk-rGO scaffold was decreased as almost 46.6% due to the incorporation of rGo to the structure of the scaffold.…”

Section: Discussionmentioning

confidence: 99%

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Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

et al. 2019

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An electrical stimulus is a new approach to neural differentiation of stem cells. In this work, the neural differentiation of conjunctiva mesenchymal stem cells (CJMSCs) on a new 3D conductive fibrous scaffold of silk fibroin (SF) and reduced graphene oxide (rGo) were examined. rGo (3.5% w/w) was dispersed in SF‐acid formic solution (10% w/v) and conductive nanofibrous scaffold was fabricated using the electrospinning method. SEM and TEM microscopies were used for fibrous scaffold characterization. CJMSCs were cultured on the scaffold and 2 electrical impulse models (Current 1:115 V/m, 100‐Hz frequency and current 2:115 v/m voltages, 0.1‐Hz frequency) were applied for 7 days. Also, the effect of the fibrous scaffold and electrical impulses on cell viability and neural gene expression were examined using MTT assay and qPCR analysis. Fibrous scaffold with the 220 ± 20 nm diameter and good dispersion of graphene nanosheets at the surface of nanofibers were fabricated. The MTT result showed the viability of cells on the scaffold, with current 2 lower than current 1. qPCR analysis confirmed that the expression of β‐tubulin (2.4‐fold P ≤ 0.026), MAP‐2 (1.48‐fold; P ≤ 0.03), and nestin (1.5‐fold; P ≤ 0.03) genes were higher in CJMSCs on conductive scaffold with 100‐Hz frequency compared to 0.1‐Hz frequency. Collectively, we proposed that SF‐rGo fibrous scaffolds, as a new conductive fibrous scaffold with electrical stimulation are good strategies for neural differentiation of stem cells and the type of electrical pulses has an influence on neural differentiation and proliferation of CJMSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

et al. 2019

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“…Electrospun silk mats (much like silk meshes) are not conductive (<10 −14 S cm −1 ) . However, the electrical conductivity can be increased by coating/adsorbing/doping with carbon nanotubes (see Figure c,d for an image and electrical characterization of the modified silk mat), blending with graphene solutions or coating with polypyrrole, up to a conductivity of ≈10 −4 S cm −1 .…”

Section: Biomolecular Electronic Materials At the Macroscalementioning

confidence: 99%

Macroscale Biomolecular Electronics and Ionics

2018

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www.advmat.de www.advancedsciencenews.com modern semiconductor-based electronic processing. The melanin story is also an archetype of the more recent "DNA-debate" and questions as to whether proteins, lipids, polysaccharides, etc., can intrinsically sustain electronic conduction and hence ET in the solid state. [13,31,32,41] That said, it is now appropriate and timely to introduce the basic concepts of ionic conduction.

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“…), [ 12,17–20 ] and a variety of stimuli‐responsive silk‐based materials have been reported. [ 21–28 ]…”

Section: Figurementioning

confidence: 99%

Electroactive Silk Fibroin Films for Electrochemically Enhanced Delivery of Drugs

Mousavi

Harper

Municoy

et al. 2020

Macro Materials & Eng

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Biomaterials capable of controlling the delivery of drugs have the potential to treat a variety of conditions. Herein, the preparation of electrically conductive silk fibroin film‐based drug delivery devices is described. Casting aqueous solutions of Bombyx mori silk fibroin, followed by drying and annealing to impart β‐sheets to the silk fibroin, assure that the materials are stable for further processing in water; and the silk fibroin films are rendered conductive by generating an interpenetrating network of a copolymer of pyrrole and 3‐amino‐4‐hydroxybenzenesulfonic acid in the silk fibroin matrix (characterized by a variety of techniques including circular dichroism, Fourier‐transform infrared spectroscopy, nuclear magnetic resonance, Raman spectroscopy, resistance measurements, scanning electron microscopy‐energy dispersive X‐ray spectroscopy, thermogravimetric analysis, X‐ray diffraction, and X‐ray photoelectron spectroscopy). Fibroblasts adhere on the surface of the biomaterials (viability assessed using an (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) assay and visualized using a confocal microscope), and a fluorescently labeled drug (Texas‐Red Gentamicin) can be loaded electrochemically and released (µg cm−2 quantities) in response to the application of an electrical stimulus.

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Preparation and characterization of electrospun graphene/silk fibroin conductive fibrous scaffolds

Abstract: A conductive fibrous scaffold made of silk fibroin and graphene was developed using electrospinning technique. The 3% G/SF scaffolds showed improved electroactivity and mechanical properties. Moreover, they could support the cell growth in vitro.

Cited by 41 publications

References 43 publications

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

Macroscale Biomolecular Electronics and Ionics

Electroactive Silk Fibroin Films for Electrochemically Enhanced Delivery of Drugs

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