Preparation and characterization of gadolinium-loaded PLGA particles surface modified with RGDS for the detection of thrombus

Zhang, Yu; Zhou, Jun; Guo, Dajing; Meng, Aimin; Zheng, Yuanyi; Wang, Zhigang

doi:10.2147/ijn.s49835

Cited by 10 publications

(4 citation statements)

References 30 publications

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“…Due to its better affinity for PLGA, the drug encapsulation and loading efficiencies of lipophilic GdDO3AC 12 were largely increased compared to previous attempts with GdDOTA [34]. Indeed, the Gd amount per NP was comparable with similar [15,35,36] or even more hydrophilic nanosystems [37][38][39]. This is an interesting result, as it was obtained through the simple hydrophobic interaction within the polymer matrix.…”

Section: Physicochemical and Morphological Characteristics Of Gddo3ac...mentioning

confidence: 84%

A new magnetic resonance imaging contrast agent loaded into poly(lacide-co-glycolide) nanoparticles for long-term detection of tumors

et al. 2014

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The incorporation of a lipophilic Gd chelate (GdDO3A-C12) in biocompatible PLGA poly(D, L-lactide-co-glycolide) nanoparticles was explored as an approach to increase the relaxivity of contrast agents for magnetic resonance imaging. By nanoprecipitation, it was possible to obtain PEGylated gadolinium nanoparticles (mean diameter of 155 nm) with high Gd loading (1.1 × 10(4) Gd centers per nanoparticle). The corresponding GdDO3AC12 ⊂ NPs nanoparticles exhibited an enhanced relaxivity (up to sixfold greater than DOTAREM® at 40 MHz) because the nanoparticle framework constrained the lipophilic Gd chelate motion and favorably impacted the Gd chelate rotational correlation time. T1-weighted imaging at 3 T on phantoms showed enhanced contrast for the GdDO3AC12 ⊂ NPs. Importantly, Gd chelate leakage was almost nonexistent, which suggested that these GdDO3AC12 ⊂ NPs could be useful for long-term MRI detection.

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Section: Physicochemical and Morphological Characteristics Of Gddo3ac...mentioning

confidence: 84%

A new magnetic resonance imaging contrast agent loaded into poly(lacide-co-glycolide) nanoparticles for long-term detection of tumors

et al. 2014

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“…Thrombus-targeted MRI can facilitate the identification of thrombi in the context of atherosclerotic plaques, with particular imaging targets enabling radiographic differentiation between newer active thrombi and older thrombi ( 86 ). Several MR contrast agents with shortening ability of T1, T2, or T2 * relaxation times, a long half-life, and satisfactory safety profiles have been developed including Gd-DTPA, superparamagnetic iron oxide (SPIO), and ultra-small superparamagnetic iron oxide particles (USPIOs) ( 87 ). In one recent report, researchers designed ultra-small magnetic dual contrast iron oxide nanoparticles (DCIONs) that had been functionalized via conjugation to activated platelet-specific scFv antibody fragments.…”

Section: Molecular Imaging Of Thrombosismentioning

confidence: 99%

Molecular Imaging and Non-molecular Imaging of Atherosclerotic Plaque Thrombosis

Guo

et al. 2021

Front. Cardiovasc. Med.

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Thrombosis in the context of atherosclerosis typically results in life-threatening consequences, including acute coronary events and ischemic stroke. As such, early detection and treatment of thrombosis in atherosclerosis patients is essential. Clinical diagnosis of thrombosis in these patients is typically based upon a combination of imaging approaches. However, conventional imaging modalities primarily focus on assessing the anatomical structure and physiological function, severely constraining their ability to detect early thrombus formation or the processes underlying such pathology. Recently, however, novel molecular and non-molecular imaging strategies have been developed to assess thrombus composition and activity at the molecular and cellular levels more accurately. These approaches have been successfully used to markedly reduce rates of atherothrombotic events in patients suffering from acute coronary syndrome (ACS) by facilitating simultaneous diagnosis and personalized treatment of thrombosis. Moreover, these modalities allow monitoring of plaque condition for preventing plaque rupture and associated adverse cardiovascular events in such patients. Sustained developments in molecular and non-molecular imaging technologies have enabled the increasingly specific and sensitive diagnosis of atherothrombosis in animal studies and clinical settings, making these technologies invaluable to patients' health in the future. In the present review, we discuss current progress regarding the non-molecular and molecular imaging of thrombosis in different animal studies and atherosclerotic patients.

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“…The high shear stress of arterial blood flow is also an important factor. To improve the specificity and stability of the binding of the NPs to thrombi, several methods have been reported: using phase-change materials to prepare NPs to increase the contact area with the thrombi,9,10 using acoustic radiation force to make the NPs aggregate and slow down near the vascular wall,11 using specific interactions between ligands and receptors enriched in the target tissues5,7,12,13 or even using two pairs of ligand-receptor modes14,15 to increase the adhesion ability of NPs to the targets.…”

Section: Introductionmentioning

confidence: 99%

“…One of the most commonly used ligands is RGD/cyclic RGD (cRGD),5,7,16 a class of short peptides containing arginine-glycine-aspartic acid (Arg-Gly-Asp), for targeting the platelet membrane glycoprotein IIb/IIIa (GPIIb/IIIa, also known as integrin αIIbβ3). However, Merten and his colleagues17 found that the maximum activation of GPIIb/IIIa occurred in the first 10 s and the GPIIb/IIIa-fibrinogen complex would affect the binding of GPIIb/IIIa with molecular probes carrying the RGD peptide.…”

Section: Introductionmentioning

confidence: 99%

<p>Polydopamine-modified dual-ligand nanoparticles as highly effective and targeted magnetic resonance/photoacoustic dual-modality thrombus imaging agents</p>

Zhong¹,

Ye²,

Xu³

et al. 2019

IJN

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Background Platelet activation and subsequent aggregation are the initial stages of thrombosis. A molecular probe that specifically targets activated platelets and remains retained under high shear stress in vivo can enhance the imaging effect to achieve early and accurate diagnosis. Methods and materials In this study, we constructed nanoparticles (NPs) using polydopamine to carry two peptides that simultaneously bind integrin αIIbβ3 and P-selectin on activated platelets to enhance the targeting of NPs to thrombus. Results The targeting specificity and binding stability of the NPs on red and white thrombi were demonstrated in vitro using a simulated circulatory device and the targeting effect of the NPs on mixed thrombus was studied by magnetic resonance (MR)/photoacoustic (PA) dual-modality imaging in vivo. NPs that were surface modified with both peptides have higher selectivity and retention to red and white thrombi in vitro than NPs with a single or no peptide, and the targeting effect was closely related to the number and distribution of activated platelets as well as the structure and type of thrombus. The NPs also have MR/PA dual-modality imaging functionality, significantly enhancing the imaging of mixed thrombus in vivo. Conclusion These dual-targeted NPs have improved targeting specificity and binding stability to different thrombi under high shear stress and are beneficial for the early diagnosis of thrombosis.

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Preparation and characterization of gadolinium-loaded PLGA particles surface modified with RGDS for the detection of thrombus

Cited by 10 publications

References 30 publications

A new magnetic resonance imaging contrast agent loaded into poly(lacide-co-glycolide) nanoparticles for long-term detection of tumors

A new magnetic resonance imaging contrast agent loaded into poly(lacide-co-glycolide) nanoparticles for long-term detection of tumors

Molecular Imaging and Non-molecular Imaging of Atherosclerotic Plaque Thrombosis

<p>Polydopamine-modified dual-ligand nanoparticles as highly effective and targeted magnetic resonance/photoacoustic dual-modality thrombus imaging agents</p>

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