Preparation and characterization of gelatin/α-TCP/SF biocomposite scaffold for bone tissue regeneration

Huh, JunTae; Lee, JiUn; Kim, Won Jin; Yeo, Miji; Kim, GeunHyung

doi:10.1016/j.ijbiomac.2017.09.030

Cited by 48 publications

(17 citation statements)

References 52 publications

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“…In addition, the effect of morphology on the mechanical properties of the composite scaffolds was assessed, implying that double layer staggered orthogonal scaffolds (GEHA20-ZZ and GEHA20-ZZS) demonstrated higher compressive strength compared with the other scaffolds. Based on these results, the composite scaffolds fabricated in this study exhibited similar compressive strength to general gelatin-based composite scaffolds for bone tissue engineering [25,26]. The crystalline phases of the composite scaffolds were measured via X-ray diffraction (XRD), as shown in Figure 6c.…”

Section: Fabrication and Characterization Of The 3d Composite Scaffoldsmentioning

confidence: 73%

Effect of Morphological Characteristics and Biomineralization of 3D-Printed Gelatin/Hyaluronic Acid/Hydroxyapatite Composite Scaffolds on Bone Tissue Regeneration

Kim

Han

Lee

et al. 2021

IJMS

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The use of porous three-dimensional (3D) composite scaffolds has attracted great attention in bone tissue engineering applications because they closely simulate the major features of the natural extracellular matrix (ECM) of bone. This study aimed to prepare biomimetic composite scaffolds via a simple 3D printing of gelatin/hyaluronic acid (HA)/hydroxyapatite (HAp) and subsequent biomineralization for improved bone tissue regeneration. The resulting scaffolds exhibited uniform structure and homogeneous pore distribution. In addition, the microstructures of the composite scaffolds showed an ECM-mimetic structure with a wrinkled internal surface and a porous hierarchical architecture. The results of bioactivity assays proved that the morphological characteristics and biomineralization of the composite scaffolds influenced cell proliferation and osteogenic differentiation. In particular, the biomineralized gelatin/HA/HAp composite scaffolds with double-layer staggered orthogonal (GEHA20-ZZS) and double-layer alternative structure (GEHA20-45S) showed higher bioactivity than other scaffolds. According to these results, biomineralization has a great influence on the biological activity of cells. Hence, the biomineralized composite scaffolds can be used as new bone scaffolds in bone regeneration.

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Section: Fabrication and Characterization Of The 3d Composite Scaffoldsmentioning

confidence: 73%

Effect of Morphological Characteristics and Biomineralization of 3D-Printed Gelatin/Hyaluronic Acid/Hydroxyapatite Composite Scaffolds on Bone Tissue Regeneration

Kim

Han

Lee

et al. 2021

IJMS

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“…Numerous attempts have been made to design an artificial scaffold that is homogeneous and biocompatible for in vitro regeneration of new bone tissues (4,5). A well-designed scaffold derived from natural or synthetic polymer must fulfill specific requirements to support cell growth (6)(7)(8)(9). First, the scaffold must be osteoconductive, allowing bone cells to attach to and proliferate on its surface (10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Skeletal microenvironment system utilising bovine bone scaffold co‑cultured with human osteoblasts and osteoclast‑like cells

et al. 2021

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“…Huh et al enhanced the osteoblast‐like cells interaction with the gelatin/tricalcium phosphate/(silk‐fibroin) using adequate mechanical properties (Huh, Lee, Kim, Yeo, & Kim, ).…”

Section: Introductionmentioning

confidence: 99%

Fabrication, characterization, and in vitro evaluation of electrospun polyurethane‐gelatin‐carbon nanotube scaffolds for cardiovascular tissue engineering applications

2020

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Myocardial infarction occurs because coronary arteries insufficiency is one of the major causes of mortality worldwide. Recent studies have shown that tissue engineering of myocardial tissue to regenerate infarcted tissue or engineering of the coronary artery may help overcome this problem. In the present research, gelatin and single-walled carbon nanotube were firstly administrated to physico-chemically and biologically modulate polyurethane nanofibers. Electrospinning, as versatile and effective technique for production of functional nanoscale fiber, was applied. Incorporation of both gelatin and SWNTs reduced mean diameter of nanofibrous scaffolds from 210 to 140 nm, which influenced on initial cell behavior. Possible interaction between gelatin and SWNTs with polyurethane chains was evaluated using FTIR and DSC techniques. Regarding the incorporation of both gelatin and SWNTs, it was found that hydrophilicity of nanofibrous scaffolds dramatically improved. Scaffold degradation profile was adjusted by incorporation of gelatin. Biomimetic mechanical properties of composite scaffolds like normal blood vessel were developed and SWNTs improved the Young modulus and ultimate strength of scaffolds up to 16.47 ± 0.5 and 23.73 ± 0.5 MPa, respectively. However, addition of gelatin increased elongation at break due to its softening effect. The incorporation of the SWNTs led to significant enhancement of electrical conductivity of the scaffolds. Biological evaluation using SEM and MTT assay demonstrated that nanofibrous surface was covered by confluent and dense layer of both myocardial myoblast and endothelial cells after 7 days of culture, which is crucial for cardiovascular tissue engineering. Results verified that the fabricated scaffolds could be effective for cardiovascular tissue engineering.

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Preparation and characterization of gelatin/α-TCP/SF biocomposite scaffold for bone tissue regeneration

Cited by 48 publications

References 52 publications

Effect of Morphological Characteristics and Biomineralization of 3D-Printed Gelatin/Hyaluronic Acid/Hydroxyapatite Composite Scaffolds on Bone Tissue Regeneration

Effect of Morphological Characteristics and Biomineralization of 3D-Printed Gelatin/Hyaluronic Acid/Hydroxyapatite Composite Scaffolds on Bone Tissue Regeneration

Skeletal microenvironment system utilising bovine bone scaffold co‑cultured with human osteoblasts and osteoclast‑like cells

Fabrication, characterization, and in vitro evaluation of electrospun polyurethane‐gelatin‐carbon nanotube scaffolds for cardiovascular tissue engineering applications

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