Preparation and characterization of immobilized lipase on magnetic hydrophobic microspheres

Guo, Zheng; Bai, Shu; Sun, Yan

doi:10.1016/s0141-0229(03)00051-6

Cited by 122 publications

(57 citation statements)

References 26 publications

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“…Immobilized enzymes often have other drawbacks such as low rates of enzyme recovery and recycling [12,[30][31][32][33]. However, it has been proven that the use of magnetic carriers can dramatically improve the efficiency of enzyme recovery and recycling [34][35][36]. Thus, the magnetically separable Lac-PpPD/Fe 3 O 4 nanocomposite may contribute to enzyme recovery and reduce costs.…”

Section: Effects Of Ph and Temperature On Lac Enzyme Activitymentioning

confidence: 99%

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

et al. 2016

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Magnetic poly(p-phenylenediamine) (PpPD) nanocomposite was synthesized via mixing p-phenylenediamine solution and Fe 3 O 4 nanoparticles and used as a carrier for immobilized enzymes. Successful synthesis of PpPD/Fe 3 O 4 nanofiber was confirmed by transmission electron microscopy and Fourier transform infrared spectroscopy. Laccase (Lac) was immobilized on the surface of PpPD/Fe 3 O 4 nanofiber through covalent bonding for reactive blue 19 dye removal. The immobilized Lac-nanofiber conjugates could be recovered from the reaction solution using a magnet. The optimum reaction pH and temperature for the immobilized Lac were 3.5 and 65 • C, respectively. The storage, operational stability, and thermal stability of the immobilized Lac were higher than those of its free counterpart. The dye removal efficiency of immobilized Lac was about 80% in the first 1 h of incubation, while that of free Lac was about 20%. It was found that the unique electronic properties of PpPD might underlie the high dye removal efficiency of immobilized Lac. Over a period of repeated operation, the dye removal efficiency was above 90% during the first two cycles and remained at about 43% after eight cycles. Immobilized Lac on PpPD/Fe 3 O 4 nanofiber showed high stability, easy recovery, reuse capabilities, and a high removal efficiency for reactive blue 19 dye; therefore, it provides an optional tool for dye removal from wastewater.

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Section: Effects Of Ph and Temperature On Lac Enzyme Activitymentioning

confidence: 99%

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

et al. 2016

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“…For instance, coupling reactions can be performed using reactive groups such as amines, carboxylates and thiols. Carboxylic groups can be activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, or EDC (Kondo and Fukuda 1997) in order to bind amines, yielding stable amide bonds linking the enzyme to the support (Bílková et al 2002, Hong et al 2007, Demirel et al 2004, Demirel and Mutlu 2005, Zeng et al 2006, Guo et al 2003, Iman et al 1992, Pan et al 2009). Amine groups can be linked by means of glutaraldehyde, forming imine bonds, which can further be reduced with NaBH 4 .…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

Carbon dioxide/methanol conversion cycle based on cascade enzymatic reactions supported on superparamagnetic nanoparticles

Netto

Andrade

Toma

2017

An. Acad. Bras. Ciênc.

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The conversion of carbon dioxide into important industrial feedstock is a subject of growing interest in modern society. A possible way to achieve this goal is by carrying out the CO 2 /methanol cascade reaction, allowing the recycle of CO 2 using either chemical catalysts or enzymes. Efficient and selective reactions can be performed by enzymes; however, due to their low stability, immobilization protocols are required to improve their performance. The cascade reaction to reduce carbon dioxide into methanol has been explored by the authors, using, sequentially, alcohol dehydrogenase (ADH), formaldehyde dehydrogenase (FalDH), and formate dehydrogenase (FDH), powered by NAD + /NADH and glutamate dehydrogenase (GDH) as the co-enzyme regenerating system. All the enzymes have been immobilized on functionalized magnetite nanoparticles, and their reactions investigated separately in order to establish the best performance conditions. Although the stepwise scheme led to only 2.3% yield of methanol per NADH; in a batch system under CO 2 pressure, the combination of the four immobilized enzymes increased the methanol yield by 64 fold. The studies indicated a successful regeneration of NADH in situ, envisaging a real possibility of using immobilized enzymes to perform the cascade CO 2 -methanol reaction.

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“…A síntese do material magnético foi realizada pela técnica de co-precipitação baseada nos trabalhos de Koneracká et al [11] e Guo et al [12] com modificações. Em dois béqueres de 100 mL foram pesados 27 g de cloreto férrico (FeCl 3 ) (Vetec, RJ, Brasil) e 14 g de sulfato ferroso (FeSO 4 ) (Vetec, RJ, Brasil).…”

Section: Preparação Do Materials Magnéticounclassified

Microesferas magnéticas à base de poli(metacrilato de metila-co-divinilbenzeno) obtidas por polimerização em suspensão

et al. 2015

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ResumoMicroesferas poliméricas à base de metacrilato de metila (MMA), divinilbenzeno (DVB) e material magnético foram preparadas via polimerização por suspensão. Foi estudada a influência da polimerização em suspensão ou semisuspensão, concentração de material magnético e concentração de divinilbenzeno sobre as características das microesferas obtidas. As partículas poliméricas foram caracterizadas por espectroscopia vibracional na região do infravermelho por transformada de Fourier (FT-IR), microscopia eletrônica de varredura, analisador de área específica e porosimetria, espalhamento de luz e magnetometria de amostra vibrante. O material magnético também foi caracterizado por FT-IR, além de difratometria de raios X e espectroscopia Mössbauer. Foram obtidas, com sucesso, microesferas poliméricas magnéticas à base de MMA e DVB (P(MMA-co-DVB)-M), contendo partículas magnéticas tanto na superfície quanto no interior da microesfera. As partículas obtidas através de polimerização em semisuspensão apresentaram tamanhos menores e distribuição de tamanhos de partículas mais estreita que as partículas obtidas através de suspensões convencionais. Os compósitos apresentaram um comportamento superparamagnético. As microesferas magnéticas sintetizadas neste trabalho têm potencial para serem modificadas e aplicadas como resinas de troca iônica ou suportes catalíticos. Palavras-chave: polimerização em suspensão, propriedades magnéticas, metacrilato de metila, divinilbenzeno, material magnético. AbstractMagnetic polymeric microspheres based on methyl methacrylate (MMA), divinylbenzene (DVB) and magnetic materials were prepared via suspension polymerization. We studied the influence of the suspension or semi-suspension polymerization, magnetic materials and divinylbenzene concentration on the characteristics of the microspheres. Polymeric particles were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy, accelerated surface area and porosimetry analyzer (ASAP), dynamic light scattering (DLS) and vibrating sample magnetometer (VSM). The magnetic materials were also characterized by FT-IR, X-ray diffraction and Mössbauer spectroscopy. Magnetic polymeric microspheres based on MMA and DVB (P(MMA-co-DVB)-M), containing magnetic particles on the surface or inside the microspheres, were successfully obtained. The polymer particles obtained through semi-suspension polymerization had smaller sizes and narrower particle size distribution than particles obtained by conventional suspension. The composites showed a superparamagnetic behavior. The magnetic microspheres synthesized in this work have potential to be modified and applied as ion-exchange resins or catalyst supports.

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Preparation and characterization of immobilized lipase on magnetic hydrophobic microspheres

Cited by 122 publications

References 26 publications

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

Carbon dioxide/methanol conversion cycle based on cascade enzymatic reactions supported on superparamagnetic nanoparticles

Microesferas magnéticas à base de poli(metacrilato de metila-co-divinilbenzeno) obtidas por polimerização em suspensão

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