Preparation and Characterization of Innovative Protein-coated Poly(Methylmethacrylate) Core-shell Nanoparticles for Vaccine Purposes

Abstract

This study demonstrates that these new nanoparticles provide a versatile platform for protein surface adsorption and a promising delivery system particularly when the maintenance of the biologically active conformation is required for vaccine efficacy.

HIV-1 Tat-Based Vaccines: An Overview and Perspectives in the Field of HIV/AIDS Vaccine Development

“…Caputo et al multimerization and loss of biological activity. Notably, Tat protein vaccine formulations, including these core-shell nano-and microparticles, were well-tolerated and not reactogenic in mice after multiple injections by systemic and mucosal routes and enhanced the anti-Tat humoral and cellular responses, including CTLs [192][193][194][195]Caputo et al,submitted]. In addition, one microsphere sample loaded with the HIV-1 Tat protein was further characterized in cynomolgus macaques, confirming the safety and vaccine potency of this delivery system and new vaccine formulation [Titti et al, manuscript in preparation].…”

Nanoparticle-Based Nicotine Vaccine

“…Since NPs can encapsulate or be decorated with protein, peptide, and haptens along with other immune-potentiating components, they may be viewed by the immune system as real pathogens (Mamo and Poland 2012 ). Over the course of recent wide-ranging studies by many research groups, no signifi cant safety or toxicity concerns have been detected (Mahapatro and Singh 2011 ;Galloway et al 2013 ;Su et al 2011 ;Taha et al 2012 ;Voltan et al 2007 ). It is apparent that synthetic polymer-based platforms may provide augmented immunogenicity by efficient adjuvant delivery, surface exposure of conserved antigens, and T-cell response induction (Bachmann and Jennings 2010 ;Jennings and Bachmann 2009 ).…”

Nasal Delivery of Biopharmaceuticals

“…Eudragit-coated PMMA NPs were developed as a delivery system for protein vaccine candidates. The NPs were shown to deliver and release HIV-1 Tat intracellularly and efficiently, protect it from oxidation, and preserve its biological activity, thereby increasing its shelf life, which is particularly noteworthy for vaccine applications [117]. Polypropylene sulfide (PPS) NPs conjugated with OVA and the TLR5 ligand flagellin were developed as a platform for nasal vaccination [118].…”