Preparation and Characterization of Iron Oxide–Silica Composite Particles Using Mesoporous SBA-15 Silica as Template and Their Internalization Into Mesenchymal Stem Cell and Human Bone Cell Lines

Abstract

A new procedure for preparing iron oxide-silica nanocomposite particles using SBA-15 mesoporous silica as a template is described. These composite materials retained the 2-D hexagonal structure of the SBA-15 template. Transmission electron micrograms of the particles depicted the formation of iron oxide nanocrystals inside the mesochannels of SBA-15 silica framework. Powder x-ray diffraction showed that the iron oxide core of the composite particles consists of a mixture of maghemite (gamma-Fe(2)O(3)) and heamatite (alpha-Fe(2)O(3)), which is the predominant component. Superconducting quantum interference device (SQUID) magnetometry studies showed that these iron oxide-silica composite materials exhibit superparamagnetic properties. On increasing the iron oxide content, the composite particles exhibited a stronger response to magnetic fields but a less homogeneous core, with some large iron oxide particles which were thought to be formed outside the mesochannels of the SBA-15 template. Internalization of these particles into human cell lines (mesenchymal stem cells and human bone cells), which indicates their potential in medicine and biotechnology, is also discussed.

Coordinative Crystallization of ZrSiO4 and α-Fe2O3 Composites and Their Resultant Structural, Morphological, and Mechanical Characteristics

“…The positive role of silica (SiO 2 ) in bone integration and cell proliferation and the role of Fe 2 O 3 in cytotoxicity has been reported in the literature. 36,37 Nevertheless, further detailed studies are required to understand the cytotoxicity mechanisms of ZrSiO 4 −α-Fe 2 O 3 composites. Morphological Features.…”

Synthesis, characterization, and optimization of α‐Fe2O3/silica nanocomposites using homogenous precipitation method

“…As shown in Figure (c), by increasing the calcination temperature, obvious peaks pertinent to α‐Fe 2 O 3 became appear. Therefore, it could be said that iron oxide amorphous phase or nanocrystalline iron oxide particles with diameter lower than five nanometer were present in final product . Also, using Scherrer equation, the crystallite size of 10 wt% nanocomposites calcined at 500 and 700°C were determined to be about 10 and 18 nm, respectively.…”

Orthopaedic applications of nanoparticle-based stem cell therapies

“…Kostura and colleagues found that Feridex poly- L -lysine in fact inhibited chondrogenesis, yet this was not found with Feridex protamine [54,55]. Therefore, the effect of the transfection agent must be investigated further as it may interfere with the activities of the cell [56]. To overcome the limitations incurred by internalisation and the use of transfection agents, a technique known as magnetoelectroporation has been introduced [30].…”