Preparation and characterization of lung-targeting ceftiofur-loaded gelatin microspheres

Hao, Zhihui; Qu, Baihua; Wang, Yanling; Tang, Shusheng; Wang, Gang; Qiu, Mei; Zhang, Ruili; Liu, Yuanyuan; Xiao, Xia

doi:10.3109/03639045.2011.584192

Cited by 13 publications

(7 citation statements)

References 9 publications

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“…Burst release of approximately 5.0% was observed upon incubation of the nanoformulation to the PBS pH 7.4 inferring that only small fraction of loaded S6S is associated with the surface of the GNC, while the majority of S6S is within the gelatin matrix of formed GNCs [87]. A sustained release of loaded bioactive from gelatin nanoparticles was also observed by earlier investigators, and our results are in agreement with the existing reports [69, 88]. …”

Section: Resultssupporting

confidence: 92%

STAT6 siRNA Matrix-Loaded Gelatin Nanocarriers: Formulation, Characterization, andEx VivoProof of Concept Using Adenocarcinoma Cells

Youngren

Tekade

Gustilo

et al. 2013

BioMed Research International

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The clinical utility of siRNA therapy has been hampered due to poor cell penetration, nonspecific effects, rapid degradation, and short half-life. We herewith proposed the formulation development of STAT6 siRNA (S6S) nanotherapeutic agent by encapsulating them within gelatin nanocarriers (GNC). The prepared nanoformulation was characterized for size, charge, loading efficiency, release kinetics, stability, cytotoxicity, and gene silencing assay. The stability of S6S-GNC was also assessed under conditions of varying pH, serum level, and using electrophoretic assays. In vitro cytotoxicity performance was evaluated in human adenocarcinoma A549 cells following MTT assay. The developed formulation resulted in an average particle size, surface charge, and encapsulation efficiency as 70 ± 6.5 nm, +10 ± 1.5 mV, and 85 ± 4.0%, respectively. S6S-GNC showed an insignificant (P < 0.05) change in the size and charge in the presence of buffer solutions (pH 6.4 to 8.4) and FBS (10% v/v). A549 cells were treated with native S6S, S6S-lipofectamine, placebo-GNC, and S6S-GNC using untreated cells as a control. It was observed that cell viability was decreased significantly with S6S-GNC by 55 ± 4.1% (P < 0.001) compared to native S6S (2.0 ± 0.55%) and S6S-lipofectamine complex (40 ± 3.1%). This investigation infers that gelatin polymer-based nanocarriers are a robust, stable, and biocompatible strategy for the delivery of siRNA.

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Section: Resultssupporting

confidence: 92%

STAT6 siRNA Matrix-Loaded Gelatin Nanocarriers: Formulation, Characterization, andEx VivoProof of Concept Using Adenocarcinoma Cells

Youngren

Tekade

Gustilo

et al. 2013

BioMed Research International

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“…As reported previously, particles in the size range 7-28 mm are more likely to accumulate or deposit in the lungs by mechanical ltration through the capillary bed of the lung aer intravenous administration. 98,99 Hao et al 100 prepared and demonstrated the efficiency of ceiofur-loaded gelatin microspheres with a mean diameter of 21.26 mm as a LT-DDS. Organ distribution pattern studies have shown a higher accumulation of the drug in the lungs than in any other organ (e.g.…”

Section: Effect Of Size Of Drug Delivery Vehicles On Lung-targeted In...mentioning

confidence: 99%

Role of size of drug delivery carriers for pulmonary and intravenous administration with emphasis on cancer therapeutics and lung-targeted drug delivery

et al. 2014

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The design of a drug delivery system and the fabrication of efficient, successful, and targeted drug carriers are two separate issues that require slightly different design parameters. The geometry of the drug carrier such as its size and shape, chemical structure, surface chemistry, and surface charge are among the key parameters that need to be optimized to achieve the desired therapeutic behaviour.We review here the effects of the size of the drug delivery carrier on its biodistribution, target specificity, body clearance rate and, most importantly, the therapeutic action of the drug. Pulmonary and intravenous drug administration are the main focus, with special emphasis on cancer therapeutics and lung-targeted drug delivery. The significance of the effect of the dimensional variations and size of the drug delivery carriers on appropriate controlled and targeted drug delivery is explored with the aim of both prohibiting excessive therapeutic loss via different clearance routes and overcoming sideeffects and toxicity.

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“…There are many reports that focus on the preparation of lung-targeted microspheres loaded with antibiotics, anticancer drugs, or glucocorticoids for the successful treatment of tuberculosis, tumors, pneumonia, and lung injury [17–20]. However, few studies reported the preparation of lung targeting and sustained drug delivery microspheres for the treatment of lung fibrosis.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Lung-Targeting, Emodin-Loaded Polylactic Acid Microspheres and Their Properties

Chen

Yang

Sun

et al. 2014

IJMS

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Emodin (1,3,8-trihydroxy-6-methylanthraquinone) has been identified to have the potential to improve lung fibrosis and lung cancer. To avoid the liver and kidney toxicities and the fast metabolism of emodin, emodin-loaded polylactic acid microspheres (ED-PLA-MS) were prepared and their characteristics were studied. ED-PLA-MS were prepared by the organic phase dispersion-solvent diffusion method. By applying an orthogonal design, our results indicated that the optimal formulation was 12 mg/mL PLA, 0.5% gelatin, and an organic phase:glycerol ratio of 1:20. Using the optimal experimental conditions, the drug loading and encapsulation efficiencies were (19.0 ± 1.8)% and (62.2 ± 2.6)%, respectively. The average particle size was 9.7 ± 0.7 μm. In vitro studies indicated that the ED-PLA-MS demonstrated a well-sustained release efficacy. The microspheres delivered emodin, primarily to the lungs of mice, upon intravenous injection. It was also detected by microscopy that partial lung inflammation was observed in lung tissues and no pathological changes were found in other tissues of the ED-PLA-MS-treated animals. These results suggested that ED-PLA-MS are of potential value in treating lung diseases in animals.

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Preparation and characterization of lung-targeting ceftiofur-loaded gelatin microspheres

Abstract: Cef-MS is a promising alternative form with high lung-targeting properties for the treatment of respiratory infections.

Cited by 13 publications

References 9 publications

STAT6 siRNA Matrix-Loaded Gelatin Nanocarriers: Formulation, Characterization, andEx VivoProof of Concept Using Adenocarcinoma Cells

STAT6 siRNA Matrix-Loaded Gelatin Nanocarriers: Formulation, Characterization, andEx VivoProof of Concept Using Adenocarcinoma Cells

Role of size of drug delivery carriers for pulmonary and intravenous administration with emphasis on cancer therapeutics and lung-targeted drug delivery

Preparation of Lung-Targeting, Emodin-Loaded Polylactic Acid Microspheres and Their Properties

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